Antimicrobial Resistance in Salmonella enterica Serovar Heidelberg Isolates from Retail Meats, Including Poultry, from 2002 to 2006

Abstract

Salmonella enterica serovar Heidelberg frequently causes food-borne illness in humans. There are few data on the prevalence, antimicrobial susceptibility, and genetic diversity of Salmonella serovar Heidelberg isolates in retail meats. We compared the prevalences of Salmonella serovar Heidelberg in a sampling of 20,295 meats, including chicken breast (n ‫؍‬ 5,075), ground turkey (n ‫؍‬ 5,044), ground beef (n ‫؍‬ 5,100), and pork chops (n ‫؍‬ 5,076), collected during 2002 to 2006. Isolates were analyzed for antimicrobial susceptibility and compared genetically using pulsed-field gel electrophoresis (PFGE) and PCR for the bla CMY gene. A total of 298 Salmonella serovar Heidelberg isolates were recovered, representing 21.6% of all Salmonella serovars from retail meats. One hundred seventy-eight (59.7%) were from ground turkey, 110 (36.9%) were from chicken breast, and 10 (3.4%) were from pork chops; none was found in ground beef. One hundred ninety-eight isolates (66.4%) were resistant to at least one compound, and 49 (16.4%) were resistant to at least five compounds. Six isolates (2.0%), all from ground turkey, were resistant to at least nine antimicrobials. The highest resistance in poultry isolates was to tetracycline (39.9%), followed by streptomycin (37.8%), sulfamethoxazole (27.7%), gentamicin (25.7%), kanamycin (21.5%), ampicillin (19.8%), amoxicillin-clavulanic acid (10.4%), and ceftiofur (9.0%). All isolates were susceptible to ceftriaxone and ciprofloxacin. All ceftiofur-resistant strains carried bla CMY . PFGE using XbaI and BlnI showed that certain clones were widely dispersed in different types of meats and meat brands from different store chains in all five sampling years. These data indicate that Salmonella serovar Heidelberg is a common serovar in retail poultry meats and includes widespread clones of multidrug-resistant strains.

Diversity and antimicrobial resistance of Salmonella strains isolated from different sources in Burkina Faso

“…In this study, Salmonella serotypes, Derby, Hato, Hermannswerder, Urbana, Shangani, Tamberma, Kentucky, Muenster, Senftenberg, Shangani, Typhi and Typhimurium were MDR. This is similar to the findings of many authors, who reported that MDR Salmonella strains have been found to be of many serotypes such as Agona, Anatum, Pullorum, Schwarzengrund, Choleraesuis, Derby, Dublin, Heidelberg, Kentucky, Newport, Senftenberg, Typhimurium, and Uganda (Chen et al, 2004;Zhao et al, 2008). All S. Typhimurium reported in the present study were MDR and most of them were found to display a phenotype of resistance to ampicillin, chloramphenicol, streptomycin, sulfonamides, and tetracycline (ACSSuT).…”

Prevalence and antibiogram study of Salmonella and Staphylococcus aureus in poultry meat

“…Salmonella serovar Heidelberg are resistant to ceftiofur, streptomycin, tetracycline, sulfamethoxazole, chloramphenicol and trimethoprim-sulfamethoxazole. This strain of Salmonella derived from poultry products is specifically important due to its ability to cause extra intestinal infection like septicemia [34] .…”

First study of Salmonella in meat in Romania

“…Salmonella species are considered to be among the most important foodborne pathogens in the world and salmonellosis is still one of the most widespread foodborne bacterial illnesses in humans, with clinical manifestations ranging from asymptomatic state to severe disease [2]. The majority of infections are associated with the ingestion of contaminated foods such as poultry, beef, pork, eggs, milk, cheese, seafood, fruits, juices, and vegetables [3,4], although most infections caused by multidrugresistant Salmonella are acquired through contaminated foods of animal origin.…”