The development of antimicrobial resistance (AMR) poses serious threat to the environment, animal and human health in terms of treatment of the diseases especially zoonotic infections. The ability of microorganisms to withstand the action of antimicrobial therapy has serious consequences for treating bacterial infections and has emerged as a global health concern. Antibiotic resistance has been increased by the irrational use of antibiotics in veterinary care, agriculture and human medicine. This irrational use has made antimicrobial ineffective to treat diseases especially diseases of zoonotic importance. The phenomenon of AMR presents a serious risk to public health in addition to impairing the efficacy of medical interventions. The likelihood of spillover events is raised by the interdependence of ecosystems and the growing connection between humans and animals. The management of infectious diseases is made more challenging by the coexistence of zoonotic pathogens and antimicrobial resistance. The transboundary pathogens have infected the environment globally due to their spread and by developing antimicrobial resistance. The growing issue of antibiotic resistance, which has become a serious health hazard in practically every country in the globe, including Pakistan, has been vigorously advocated for by WHO. 'One Health' offers a variety of strategies to stop the transboundary and zoonotic spread of AMR and maintain the efficient use of antibiotics in both human and animal treatment. Resistant zoonotic pathogen strains have the potential to undermine the efficacy of current therapies, resulting in illnesses that worsen and last longer. Implementing ethical and rational use of antimicrobials, surveillance, and control strategies between human medical care, veterinary care and environmental professions can develop effective measures to reduce the threats of AMR and protect the world from this threat. WHO has started to adopt mitigation measures to have fruitful consequences and pleasant results to cope these conditions effectively.