Antimicrobial Resistance in <i>Enterococcus</i> sp Isolated from Soft Cheese in Southern Brazil

Abstract

The aim of this research was to study the incidence of antibiotic resistance in 56 Enterococcus strains isolated from dairy products. The identification of enterococci was detected by polymerase chain reaction (PCR) using specific primers to E. faecalis, E. faecium, E. gallinarum and E. casseliflavus, and antibiotic resistance was tested by the disk diffusion method. The most prevalent species was E. faecium with a rate of 58.33%, followed by 27.77% E. faecalis, 11.11% E. casseliflavus and 2.7% E. gallinarum. Distribution of resistance was found in different species. All isolates were susceptible to chloramphenicol, ampicillin, imipenem and amoxicillin/clavulanic acid. In addition, isolates resistant to tetracyclin, nalidixic acid, amikacin, erythromycin, vancomycin and cephalothin were detected. A total of 66.6% of E. faecium and 58.3% of E. faecalis strain were resistant to multiple drugs. The van(A) gene was detected in 100% of vancomycin resistant enterococci. Considering the results of our study, dairy enterococci can be considered a potential source for dissemination of antibiotic resistances.

Safety Evaluation of Enterocin Producer Enterococcus sp. Strains Isolated from Traditional Turkish Cheeses

“…It is believed that these bacteria contribute to the flavor and the aroma formation of some different European and Mediterranean cheeses. E. faecium, E. faecalis and E. durans species were frequently isolated from traditional dairy products (Giraffa, 2002;2003;Martin-Platero et al, 2009;Yogurtcu and Tuncer, 2013;Furlaneto-Maia et al, 2014). In the present study a total of 100 Enterococcus strains were isolated from various artisanal cheese samples and 11 strains were determined as enterocin producers and also characterized on species level as E. faecium (6 strains) and E. faecalis (5 strains).…”

“…All strains showed sensitivity against vancomycin but four of them were carrying vanA genes. Furlaneto-Maia et al (2014), reported that 5 Enterococcus sp. isolates harbored vanA gene, while they were susceptible to vancomycin phenotypically.…”

Antibiotic resistance and virulence genes in enterococcus strains isolated from different hospitals in Saudi Arabia

“…Enterococci are ubiquitous bacteria that have a predominant habitat in the digestive tract of humans and animals, but may be present in soil, water's surface and in plants. [1,2] Enterococci have emerged as important nosocomial pathogens over the past decade, [3,4] ranking only second to staphylococci as a leading cause of nosocomial infections, it associated as hospital infections in the Saudi Arabia, [5] and the first report of multi-drug resistance Enterococcus from Saudi Arabia was in 1993 from King Faisal Specialist Hospital, Riyadh. [6] They frequently possess several specific traits that enable them to survive in the hospital environment, colonize patients and cause infections such as bacteraemia, peritonitis, endocarditis and urinary tract, wound and device-related infections.…”

“…[6] They frequently possess several specific traits that enable them to survive in the hospital environment, colonize patients and cause infections such as bacteraemia, peritonitis, endocarditis and urinary tract, wound and device-related infections. [2,7] There are two major pathogenic species of Enterococcus in humans, E. faecalis and E. faecium, with occasional infections being caused by E. durans, E. gallinarum, E. casseliflavus, E. avium, E. hirae, E. mundtii and E. raffinosus. [8,9] In particular, Enterococcus species have emerged as multi-resistant nosocomial pathogens in immune compromised and critically ill patients.…”

Influence of optimised commercial medium on bacteriocin production by <i>Enterococcus faecium

“…Bacteriocinogenic E. faecium strain Efm20 and strain Efm22 isolated from soft cheese were grown at 37°C for 18 hours in MRS broth (Himedia) (Furlaneto-Maia, Rocha, Henrique, Giazzi, & Furlaneto, 2014;Ogaki, Rocha, Terra, Furlaneto, & Maia, 2016). L. innocua CLIP 12612 was used as indicator strain.…”