Antimicrobial resistance and species identification of staphylococci isolated from the meat of wild rabbits (Oryctolagus cuniculus) in Slovakia

Pipová, M.; Jevinová, Pavlí­na; Kmet, V.; Regecová, Ivana; Marušková, Katarína

doi:10.1007/s10344-011-0558-2

Cited by 14 publications

(13 citation statements)

References 34 publications

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“…After biochemical identification of Enterobacteriaceae genera were used MALDI TOF MS Biotyper (Brucker, Germany) of Slovak Academy of Sciences in Kosice, Institute of Animal Physiology, as previously described. [24] Antimicrobial activity…”

Section: The Biochemical and Maldi Tof Ms Biotyper Identification Of mentioning

confidence: 99%

In vitroandIn vivoantimicrobial activity of propolis on the microbiota from gastrointestinal tract of chickens

Kačániová

Rovná

Arpášová

et al. 2012

Journal of Environmental Science and Health, Part A

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The aim of this study was to examine the effect of propolis extracts on the microbial colonization of chicken gastrointestinal tract in vivo. The propolis was administered to both feed mixtures in various amounts except of the control group. The addition of 150 mg propolis to 1 kg of feed was included in the first experimental group, the addition of 450 mg.kg(-1) in the second experimental group, the addition of 600 mg.kg(-1) the third experimental group and 800 mg kg(-1) in the fourth one. The highest count of faecal enterococci was found in the third group (8.6 cfu.g(-1)) where 600 mg of propolis to 1 kg was added to the feed mixture. The highest count of lactobacilli was detected in the fourth experimental group (8.83 cfu.g(-1)) where was 800 mg of propolis added to 1 kg of feed mixture and number of Enterobacteriaceae genera count was found in control group (8.73 cfu.g(-1)). With RTQ PCR detected species from the genus Enterococcus were: E. avium, E. casseliflavus, E cecorum, E. faecalis, E. faecium, E. gallinarum, E. hirae and E. malodoratus and from genus Lactobacillus were: Lactobacillus crispatus, L. acidophilus and L. salivarius. With MALDI TOF MS Biotyper from Enterobacteriaceae genera were identified Citrobacter braakii, Raoultella ornithinolytica, Serratia fonticola, Escherichia coli and Klebsiella oxytoca. Antimicrobial activities In vitro of six species of bacteria isolated from gastrointestinal tract of chickens were also tested. The best antimicrobial effect of Citrobacter braakii on ethanolic propolis extract in all concentrations were found.

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Section: The Biochemical and Maldi Tof Ms Biotyper Identification Of mentioning

confidence: 99%

In vitroandIn vivoantimicrobial activity of propolis on the microbiota from gastrointestinal tract of chickens

Kačániová

Rovná

Arpášová

et al. 2012

Journal of Environmental Science and Health, Part A

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“…To distinguish between pathogenic CoPS and nonpathogenic coagulase-negative species, clumping factor, DNase and hyaluronidase are of great importance. However, some of those factors were also found in clinical and food isolates of CoNS [48,[50][51][52][53]. Results of our study demonstrate the absence of coagulase, DNase and hyaluronidase production, with lecithinase activity confirmed in 38.6% and gelatinase activity confirmed in 49.1% of CoNS isolates.…”

Section: Discussionmentioning

confidence: 54%

Virulence factors and antimicrobial resistance of coagulase-negative staphylococci isolated from drinking water

et al. 2015

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Little has been published about the occurrence, speciesidentification, andpathogenicpotentialofcoagulasenegative staphylococci (CoNS) present in drinking water. In this study, ten species were identified among 57 isolates of staphylococci from 756 samples of chlorinated drinking water taken from public distribution networks in the Slovak Republic. S. warneri (37%), S. haemolyticus (23%), and S. saprophyticus ssp. saprophyticus (14%) were identified most frequently. Isolates did not produce coagulase, DNase, or hyaluronidase; production of gelatinase and lecithinase was observed in 28 and 22 isolates, respectively. Genetically encoded ability for production of enterotoxin SED was revealed in two isolates. Among ten antibiotics tested, resistance to ampicillin (66.7%), penicillin (64.9%), and erythromycin (57.9%) were observed most frequently. Resistance to gentamicin, vancomycin, or clindamycin was not confirmed. Production of β-lactamase was observed in 64.9% of isolates. Fourty-two isolates were resistant to two or more antibiotics tested, and eight isolates showed multiresistance. The presence of mecA gene was confirmed in 8 isolates, while PBP2a was revealed in 7. Two isolates of S. epidermidis were identified as methicillin-resistant (MRSE). The results demonstrate that CoNS in chlorinated drinking water may possess virulence factors and show resistance to various antibiotics. Therefore, their pathogenic potential should not be ignored.

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“…On the contrary, the same species of CoNS isolated from rabbits' meat showed high resistance e.g. to Amp, Oxa, or Pnc (14).…”

Section: Discussionmentioning

confidence: 92%

Staphylococcal Species Detected in Free-Living Trouts of East Slovakian Water Sources and their Relation to Antimicrobials

Lauková

Kandričáková

Strompfová

et al. 2013

Bulletin of the Veterinary Institute in Pulawy

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The aim of the study was to isolate and identify staphylococci from the intestinal samples of 24 trouts from East Slovakian waters. Moreover, their relation to antimicrobials was tested. The count of staphylococci in the trouts reached in average 4.0 x 101 colony forming units per gram. Twenty-two strains were identified by validated species-specific oligonucleotide array targeting the manganese-dependent superoxide dismutase-sodA gene. The identified strains were allotted to five species (Staphylococcus warneri, S. haemolyticus, S. epidermidis, S. hominis, S. pasteuri) clustered to three groups according to 16S rRNA sequences (S. epidermidis group, S. haemolyticus group, S. warneri group). These species belong to coagulase-negative staphylococci. All strains were sensitive to eight antibiotics out of 14 tested; the majority of strains were also sensitive to the remaining six antibiotics with the inhibitory zones from 13 to 41 mm. The strains were also sensitive at least to three enterocins of nine tested. Strains SW24/2, SHo 19/2, SHo20/1, SP19/1 were sensitive to eight of nine enterocins. All strains were sensitive to Ent A, P=EK13, and Ent EM41 with activity 100-6400 AU/mL. Strains SHo19/2 and SP19/1 were sensitive to Ent 2019 with activity up to 25600 AU/mL.

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Antimicrobial resistance and species identification of staphylococci isolated from the meat of wild rabbits (Oryctolagus cuniculus) in Slovakia

Cited by 14 publications

References 34 publications

In vitroandIn vivoantimicrobial activity of propolis on the microbiota from gastrointestinal tract of chickens

In vitroandIn vivoantimicrobial activity of propolis on the microbiota from gastrointestinal tract of chickens

Virulence factors and antimicrobial resistance of coagulase-negative staphylococci isolated from drinking water

Staphylococcal Species Detected in Free-Living Trouts of East Slovakian Water Sources and their Relation to Antimicrobials

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