Antimicrobial Resistance and Multilocus Sequence Typing of Vancomycin-Resistant<i>Enterococcus faecium</i>Isolated from the Chungcheong Area

Cho, Hye Hyun; Sung, Ji Youn; Kwon, Gye Cheol; Lim, Jin Sook; Koo, Sun Hoe

doi:10.5145/kjcm.2011.14.2.60

Cited by 5 publications

(3 citation statements)

References 25 publications

(37 reference statements)

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“…Cho et al in Korea reported VRE ST 192, ST 78, ST 17, and ST 414 (highest to lowest frequency) in 2011 [22]. In our study, however, VRE ST 414 was the most frequent type.…”

Section: Resultscontrasting

confidence: 61%

Identification of vancomycin-resistant enterococci clones and inter-hospital spread during an outbreak in Taiwan

Lee

Shih

et al. 2013

BMC Infect Dis

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BackgroundIn 2003, nosocomial infections caused by vancomycin-resistant enterococci (VRE) occurred rarely in Taiwan. Between 2003 and 2010, however, the average prevalence of vancomycin resistance among enterococci spp. increased from 2% to 16% in community hospitals and from 3% to 21% in medical centers of Taiwan. We used molecular methods to investigate the epidemiology of VRE in a tertiary teaching hospital in Taiwan.MethodsBetween February 2009 and February 2011, rectal samples and infection site specimens were collected from all inpatients in the nephrology ward after patient consent was obtained. VRE strain types were determined by pulsed-field gel electrophoresis (PFGE) and multilocus sequence typing (MLST).ResultsA total of 59 vanA gene-containing VRE isolates (1 per patient) were obtained; 24 originated from rectal sample surveillance of patients who exhibited no symptoms (22 Enterococcus faecium and 2 Enterococcus faecalis), and 35 had developed infections over 3 days after admission (32 E. faecium, 2 E. faecalis, and 1 Enterococcus durans). The 59 VRE isolates demonstrated vancomycin minimum inhibitory concentrations (MICs) of ≥256 μg/m. The MIC range for linezolid, tigecycline, and daptomycin was 0.25–1.5 μg/mL, 0.032–0.25 and 1–4 μg/mL, respectively. For 56 isolates, the MIC for teicoplanin was >8 μg/mL. The predominant types in the nephrology ward were MLST types 414, 78, and18 as well as PFGE types A, C, and D.ConclusionVREs are endemic in nephrology wards. MLST 414 is the most predominant strain. The increase VRE prevalence is due to cross-transmission of VRE clones ST 414,78,18 by undetected VRE carriers. Because similar VRE STs had been reported in a different hospital of Taiwan, this finding may indicate inter-hospital VRE spread in Taiwan. Active surveillance and effective infection control policies are important controlling the spread of VRE in high risk hospital zones. All endemic VRE strains are resistant to teicoplanin but are sensitive to daptomycin, linezolid, and tigecycline.

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“…Cho et al in Korea reported VRE ST 192, ST 78, ST 17, and ST 414 (highest to lowest frequency) in 2011 [22]. In our study, however, VRE ST 414 was the most frequent type.…”

Section: Resultscontrasting

confidence: 61%

Identification of vancomycin-resistant enterococci clones and inter-hospital spread during an outbreak in Taiwan

Lee

Shih

et al. 2013

BMC Infect Dis

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“…CC17 was defined by using an MLST assay and is regarded as excellent example of cumulative evolutionary processes. In the majority of the E. faecium isolates, CC17 is characterized by ampicillin and quinolone resistance as well as the presence of a putative pathogenicity island, including the esp gene [ 24 , 25 ].…”

Section: Discussionmentioning

confidence: 99%

Expression of Sme Efflux Pumps and Multilocus Sequence Typing in Clinical Isolates of Stenotrophomonas maltophilia

et al. 2012

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BackgroundStenotrophomonas maltophilia has emerged as an important opportunistic pathogen, which causes infections that are often difficult to manage because of the inherent resistance of the pathogen to a variety of antimicrobial agents. In this study, we analyzed the expressions of smeABC and smeDEF and their correlation with antimicrobial susceptibility. We also evaluated the genetic relatedness and epidemiological links among 33 isolates of S. maltophilia.MethodsIn total, 33 S. maltophilia strains were isolated from patients in a tertiary hospital in Daejeon. Minimum inhibitory concentrations (MICs) of 11 antimicrobial agents were determined by using agar dilution method and E-test (BioMérieux, France). Real-time PCR analysis was performed to evaluate the expression of the Sme efflux systems in the S. maltophilia isolates. Additionally, an epidemiological investigation was performed using multilocus sequence typing (MLST) assays.ResultsThe findings of susceptibility testing showed that the majority of the S. maltophilia isolates were resistant to β-lactams and aminoglycosides. Twenty-one clinical isolates overexpressed smeABC and showed high resistance to ciprofloxacin. Moreover, a high degree of genetic diversity was observed among the S. maltophilia isolates; 3 sequence types (STs) and 23 allelic profiles were observed.ConclusionsThe smeABC efflux pump was associated with multidrug resistance in clinical isolates of S. maltophilia. In particular, smeABC efflux pumps appear to perform an important role in ciprofloxacin resistance of S. maltophilia. The MLST scheme for S. maltophilia represents a discriminatory typing method with stable markers and is appropriate for studying population structures.

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“…Primers chosen for amplification are shown in table (1). Rapid DNA extraction method was performed, PCR amplification was done as described by Co et al 15 .…”

Section: Detection Of Vana and Vanb Genes In Vrementioning

confidence: 99%

Enterococcal Biofilm in Colonization and Disease, Correlation with Virulence Determinants and Vancomycine Resistance

Labeeb

Adnane

2018

Egyptian Journal of Medical Microbiology

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Background: Enterococci possess many virulence genes implicated in their pathogenesis. Biofilm help the organism to colonize and cause infections. Objectives: This study was set out to investigate and compare biofilm formation ability and presence of asa1 and esp genes among E. faecium and E. faecalis isolates from diverse sources. Furthermore, association between biofilm formation, esp, asa1 genes and vancomycin resistance was analyzed. Methodology: 76 pathogenic enterococcal isolates and 36 enterococcal isolates from healthy individuals were collected. All isolates were investigated for biofilm using microtitre plate, asa1and esp genes were detected by primer-specific PCR, vancomycin resistance were screened using agar method and confirmed by PCR. Results: the majority of clinical isolates (80.5%) were biofilm producers, however biofilm was detected only in 36.4% of colonizing isolates. E. faecalis (82.6%) produced biofilm more than E. faecium (36.1%), esp gene (48.5%) was presented more than asa1gene (15.2%). Virulence genes were detected in high rates among biofilm producers isolates, low vancomycin resistance rate was seen among isolates which produced slim layers. Conclusion: biofilm was detected in high rate in E. faecalis harbored esp gene. Biofilm, asa1 and esp genes were more presented among isolates from non invasive sites than invasive sites, thus aids enterococci to provoke clinical infections, lower biofilm was seen in E.faecum. However, vancomycin resistant isolates produced less slim than vancomycin sensitive.

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Antimicrobial Resistance and Multilocus Sequence Typing of Vancomycin-ResistantEnterococcus faeciumIsolated from the Chungcheong Area

Cited by 5 publications

References 25 publications

Identification of vancomycin-resistant enterococci clones and inter-hospital spread during an outbreak in Taiwan

Identification of vancomycin-resistant enterococci clones and inter-hospital spread during an outbreak in Taiwan

Expression of Sme Efflux Pumps and Multilocus Sequence Typing in Clinical Isolates of Stenotrophomonas maltophilia

Enterococcal Biofilm in Colonization and Disease, Correlation with Virulence Determinants and Vancomycine Resistance

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