Antimicrobial Resistance and Molecular Characteristics of Methicillin-Resistant &lt;i&gt;Staphylococcus aureus&lt;/i&gt; Isolates from Child Patients of High-Risk Wards in Shenzhen, China

Yang, Qin; Wen, Fuqiang; Zheng, Yuejie; Zhao, Ruizhen; Hu, Qinghua; Zhang, Renli

doi:10.7883/yoken.jjid.2016.328

Cited by 9 publications

(3 citation statements)

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“…The lukAB , whose distribution is unknown due to lack of investigation in a large number of clinical strains, was carried by 67.8% of our isolates. There is a varying carriage of pvl among MRSA, ranging from 2.3 to 50.7% in China [ 42 – 45 ]. In the present study, a relatively low prevalence of pvl -positive isolates (7.9%) was found, which was in agreement with our previous data (6.6%) [ 46 ].…”

Section: Discussionmentioning

confidence: 99%

Leukotoxin and pyrogenic toxin Superantigen gene backgrounds in bloodstream and wound Staphylococcus aureus isolates from eastern region of China

He¹,

Xu²,

Zhao³

et al. 2018

BMC Infect Dis

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BackgroundThe bicomponent leukotoxins and the pyrogenic toxin superantigens (PTSAgs) are important virulence factors of Staphylococcus aureus. It is necessary to survey the prevalence and expression of these toxin-encoding genes for understanding the possible pathogenic capacity of S. aureus to cause disease.MethodsFive leukotoxin genes and thirteen PTSAg determinants were detected for 177 S. aureus isolates from blood (n = 88) and wound (n = 89) infections by Polymerase Chain Reaction (PCR). The expression of leukotoxin ED (lukED) was determined by quantitative real-time PCR (qRT-PCR). The genetic backgrounds of isolates were analyzed by Staphylococcal Cassette Chromosome mec (SCCmec) typing (for methicillin-resistant S. aureus isolates), Pulsed-Field Gel Electrophoresis (PFGE), accessory gene regulator (agr) typing and Multilocus Sequence Typing (MLST, for representative isolates based on PFGE type) methods.Results99.4% (176/177) isolates contained at least one of leukotoxin genes. Among them, 94.9% (168/177), 81.4% (144/177) and 67.8% (120/177) isolates harbored hlgBC, lukED and lukAB, respectively. Compared to leukotoxin genes, there was a relatively lower overall prevalence of PTSAg genes [99.4% versus 72.9% (129/177), P < 0.001], and they were organized in 59 patterns, with the most common combination of the egc cluster with or without other PTSAg genes. Genetic analysis showed the distributions of certain toxin genes were associated with the genetic backgrounds of isolates. The egc cluster was a common feature of CC5 isolates, among which ST5 and ST764 isolates harbored more PTSAg genes. The lukED was not present in ST398 isolates, and its expression was quite different among isolates. No significant correlations were observed between the lukED expression levels of strains and the ST or agr types.ConclusionsThe present study elucidated the distribution of leukotoxin and PTSAg genes and the expression of lukED in blood and wound isolates, and analyzed the relationship between them with genetic characteristics of isolates. These data improve the current understanding of the possible pathogenicity of S. aureus.Electronic supplementary materialThe online version of this article (10.1186/s12879-018-3297-0) contains supplementary material, which is available to authorized users.

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Section: Discussionmentioning

confidence: 99%

Leukotoxin and pyrogenic toxin Superantigen gene backgrounds in bloodstream and wound Staphylococcus aureus isolates from eastern region of China

He¹,

Xu²,

Zhao³

et al. 2018

BMC Infect Dis

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“…In Shenzhen, the main MRSA clone was ST59-SCCmecIV-t437. 27 In Northwest China, the most common clone was ST239-SCCmecIII-t030. 28 Based on published reports, the ST398 typing is a zoonotic clonotype.…”

Section: Discussionmentioning

confidence: 99%

Molecular Epidemiology of Methicillin-Resistant Staphylococcus aureus in Hospitalized Patients in Eastern Heilongjiang Province, China

Yang¹,

Zhao²,

Wang³

et al. 2021

IDR

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Background Recently, owing to antibiotic resistance, the incidence of methicillin-resistant Staphylococcus aureus (MRSA) colonization among intensive care unit (ICU) patients has increased rapidly. So far, there are few studies on active screening of MRSA. The purpose of the current study was to verify the effectiveness of active screening and analyze the molecular epidemiological characteristics of MRSA in the region. Methods We collected 30 samples of the MRSA strains from a tertiary hospital in the Eastern Heilongjiang Province. Among them, 7 were retrieved through nasal vestibular swabs at the emergency ICU and 23 were obtained from clinical specimens. Additionally, relevant patient medical information was examined retrospectively and molecular epidemiology and risk factor analysis for MRSA were performed. Results Molecular epidemiology studies revealed that all strains of bacteria carried the mecA resistance gene. The Panton Valentine leukocidin (PVL), for instance, was detected at a rate of 13.33% (4/30). The Staphylococcus aureus protein A (spa) types, found amongst our samples, were mainly t324, t437, t034, etc., and we discovered a new spa type t19702. We also revealed 3 types of SCCmec, namely, SCCmec type II, SCCmec type IVa, and SCCmec type V, with the most prevalent clonotypes being ST72 and ST59. In addition, we also found 7 new ST types, namely, ST6567, ST6568, ST6569, ST6570, ST6571, ST6572, and ST6573. Using risk factor analysis, we also demonstrated that long, invasive procedures used in the ICU, such as tracheal intubation and ventilator usage, along with patients with cerebral infarction and other embolism are more susceptible to developing MRSA colonization and further infections. Conclusion We recommend the infection control department within hospitals to actively screen for MRSA and perform risk factor analysis in order to establish accurate preventive measures for controlling MRSA spread.

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“…MRSA устойчивы к большинству применяемых в здравоохранении антибиотиков -пенициллинам, оксациллину, эритромицину, клиндамицину и тетрациклину, реже встречается устойчивость к сульфаметоксазолу, триметоприму и гентамицину. Часть штаммов MRSA сохраняет чувствительность к цефтобипролу, цефтралиону, ванкомицину, левофлоксацину и моксифлоксацину [1]. Эту группу стафилококков отличает наличие стафилококковой хромосомной кассеты mec (staphylococcal cassette chromosome mec SCCmec).…”

Section: Introductionunclassified

Livestock-associated Methicillin-resistant Staphylococcus aureus as a New Impendence for Public Health

Vaganova

Борисенко

Sokurova

et al. 2019

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Резюме MRSA представляет собой неоднородную группу в рамках вида Staphylococcus aureus. В составе данной группы выделяют, в зависимости от объекта паразитирования, три подгруппы: внутрибольничные варианты MRSA (hospital-associated MRSA, HA-MRSA), внебольничные MRSA (community-associated MRSA, CA-MRSA), MRSA, ассоциированные с сельскохозяйственными животными (livestock-associated MRSA, LA-MRSA), распространённые среди сельскохозяйственных животных. LA-MRSA широко распространены во многих странах. Существенной проблемой является распространение носительства LA-MRSA среди лиц, контактирующих с сельскохозяйственными животными. Взаимодействие популяций CA-MRSA и LA-MRSA приводит к получению LA-MRSA генов, ассоциированных с патогенностью для человека. В результате этого процесса возникают варианты LA-MRSA, способные распространяться среди людей. Эти стафилококки обладают способностью вызывать заболевания человека, в том числе внутрибольничные инфекции. Интенсификация животноводства, обусловливающая массовое и бесконтрольное применение антибиотиков, привела к формированию и повсеместному распространению LA-MRSA, характеризующегося, в отличие от CA-MRSA, множественной лекарственной резистентностью. Это обстоятельство делает практически невозможным элиминацию LA-MRSA из среды обитания человека и животных. Данное положение даёт основание признать, что одним из основных способов контроля за распространением LA-MRSA и вызываемых им заболеваний является мониторорование экологической, эпизоотической и эпидемиологической ситуаций среди поголовья сельскохозяйственных животных и населения.

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Antimicrobial Resistance and Molecular Characteristics of Methicillin-Resistant <i>Staphylococcus aureus</i> Isolates from Child Patients of High-Risk Wards in Shenzhen, China

Cited by 9 publications

References 40 publications

Leukotoxin and pyrogenic toxin Superantigen gene backgrounds in bloodstream and wound Staphylococcus aureus isolates from eastern region of China

Leukotoxin and pyrogenic toxin Superantigen gene backgrounds in bloodstream and wound Staphylococcus aureus isolates from eastern region of China

Molecular Epidemiology of Methicillin-Resistant Staphylococcus aureus in Hospitalized Patients in Eastern Heilongjiang Province, China

Livestock-associated Methicillin-resistant Staphylococcus aureus as a New Impendence for Public Health

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