Antimicrobial resistance and genetic diversity of the SXT element in Vibrio cholerae from clinical and environmental water samples in northeastern Thailand

Mala, Wanida; Faksri, Kiatichai; Samerpitak, Kittipan; Yordpratum, Umaporn; Kaewkes, Wanlop; Tattawasart, Unchalee; Chomvarin, Chariya

doi:10.1016/j.meegid.2017.04.013

Cited by 21 publications

(14 citation statements)

References 39 publications

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“…Further study should probably also target detection of specific markers to evaluate the presence of the SXT element in this non-O1/non-O139 V. cholerae collection. But, since multiple studies investigating either clinical or non-pathogenic collections of V. cholerae (Ceccarelli et al, 2006 ; Mala et al, 2016 , 2017 ; Wang et al, 2016 ) had demonstrated that antimicrobial resistance genes carried by the SXT element are various, and sul2, strA and strB appeared to be the most commonly encountered resistance genes. We might hypothesize that the proportion of strains from our collection harboring the SXT element might be quite low.…”

Section: Discussionmentioning

confidence: 99%

Antimicrobial Susceptibility among Urban Wastewater and Wild Shellfish Isolates of Non-O1/Non-O139 Vibrio cholerae from La Rance Estuary (Brittany, France)

et al. 2017

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The early 2000s marked the end of the Golden age of the antibiotics and the beginning of the awareness on the potential threat to human health due to the dissemination of antimicrobial resistance. As a base-line study, we investigated the antimicrobial susceptibility of 99 strains of non-O1/non-O139 Vibrio cholerae isolated from wastewater and shellfish in 2000/2001 within La Rance estuary (Brittany, France). All isolates were susceptible to amoxicillin-clavulanic acid, cefotaxime, imipenem, chloramphenicol, nalidixic acid, ciprofloxacin, norfloxacin, amikacin, gentamicin, tetracycline, doxycycline, trimethoprim-sulfamethoxazole, and erythromycin. The only resistances were to streptomycin, sulfonamides and ampicillin: 54.6% of the isolates had acquired resistance to at least one antimicrobial agent among them and only six isolates from cockles were multidrug resistant. On the basis of the distribution of a limited selection of resistance associated genes, our study shows that V. cholerae can constitute an environmental reservoir for these genes. However, none of our isolates harbored integron. This result casts doubt on the capacity of non-O1/non-O139 V. cholerae to acquire resistance-associated genes in such context, and on its potential role of indicator of the dissemination of antimicrobial resistance in the aquatic environment.

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Section: Discussionmentioning

confidence: 99%

Antimicrobial Susceptibility among Urban Wastewater and Wild Shellfish Isolates of Non-O1/Non-O139 Vibrio cholerae from La Rance Estuary (Brittany, France)

et al. 2017

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“…These three integrase genes are components of Class 1 integron, Class 2 integron, and SXT element, respectively. These integrase genes are involved in the horizontal transfer of AMR genes 17,18 . IntI1 was detected in four strains (IDH‐01215, IDH‐02822, IDH‐03944, and IDH‐06641), and one strain, IDH‐06641, harbored intSXT .…”

Section: Resultsmentioning

confidence: 99%

“…The site at which IDH‐02822‐2 is located has not been identified. As GIs are usually found at the 3′ end of trmE , 18 it is likely that IDH‐02822‐2 is placed adjacent to the 3′ end of IDH‐02822‐1 and that these two GIs (IDH‐02822‐1 and IDH‐02822‐2) are arranged in tandem.…”

Section: Resultsmentioning

confidence: 99%

Genomic characterization of antibiotic resistance‐encoding genes in clinical isolates of Vibrio cholerae non‐O1/non‐O139 strains from Kolkata, India: generation of novel types of genomic islands containing plural antibiotic resistance genes

Morita¹,

Takahashi²,

Morita

et al. 2020

Microbiology and Immunology

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Non-O1/non-O139 nontoxigenic Vibrio cholerae associated with cholera-like diarrhea has been reported in Kolkata, India. However, the property involved in the pathogenicity of these strains has remained unclear. The character of 25 non-O1/non-O139 nontoxigenic V. cholerae isolated during 8 years from 2007 to 2014 in Kolkata was examined. Determination of the serogroup showed that the serogroups O6, O10, O35, O36, O39, and O70 were represented by two strains in each serogroup, and the remaining isolates belonged to different serogroups. To clarify the character of antibiotic resistance of these isolates, an antibiotic resistance test and the gene analysis were performed. According to antimicrobial drug susceptibility testing, 13 strains were classified as drug resistant. Among them, 10 strains were quinolone resistant and 6 of the 13 strains were resistant to more than three antibiotics. To define the genetic background of the antibiotic character of these strains, whole-genome ---

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“…19,26 Recentemente várias investigações abordaram e apontaram um problema real de resistência de V. cholerae O1 à tetraciclina e outros antimicrobianos em diversas áreas, predominantemente na África e Ásia. 27,28 Dessa forma, os antibióticos encurtam o curso e diminuem a gravidade da doença, mas não são tão importantes quanto a reidratação.…”

Section: Tratamento Vigilância E Recursos Em Saúdeunclassified

Vibrio cholerae: doença, manifestações clínicas e microbiologia

Moura

Muller

Gemelli

2018

Rev Epidemiol Control Infect

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Justificativa e conteúdo: A cólera é uma doença infecciosa intestinal aguda causada pela toxina do Vibrio cholerae. A transmissão é oral-fecal e ocorre predominantemente em ambientes aquáticos contaminados. Pode ser fatal, mas é facilmente evitada e tratada. Associa-se a sua propagação com a falta de saneamento básico pois cresce exponencialmente nesses ambientes. O diagnóstico é clínico-epidemiológico, laboratorial com meios seletivos para o microrganismo e teste rápido, este último não é sensível e nem especifico. O tratamento é com sais de reidratação oral e antibioticoterapia, indica-se a suplementação com zinco. Existe vacinação para Vibrio cholerae, e essa é a melhor medida para o controle da doença. As pesquisas em cólera já não são mais frequentes, pois ela é considerada uma doença de países que não são desenvolvidos. Conclusão: Nesse contexto, ainda são necessárias pesquisas sobre sistemas de esgoto, monitorização de efluentes, vigilância da qualidade da água de abastecimento público e vigilância da qualidade de alimentos.

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Antimicrobial resistance and genetic diversity of the SXT element in Vibrio cholerae from clinical and environmental water samples in northeastern Thailand

Cited by 21 publications

References 39 publications

Antimicrobial Susceptibility among Urban Wastewater and Wild Shellfish Isolates of Non-O1/Non-O139 Vibrio cholerae from La Rance Estuary (Brittany, France)

Antimicrobial Susceptibility among Urban Wastewater and Wild Shellfish Isolates of Non-O1/Non-O139 Vibrio cholerae from La Rance Estuary (Brittany, France)

Genomic characterization of antibiotic resistance‐encoding genes in clinical isolates of Vibrio cholerae non‐O1/non‐O139 strains from Kolkata, India: generation of novel types of genomic islands containing plural antibiotic resistance genes

Vibrio cholerae: doença, manifestações clínicas e microbiologia

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