Antimicrobial resistance and gene regulation in Enteroaggregative Escherichia coli from Egyptian children with diarrhoea: Similarities and differences

Abdelwahab, Radwa; Yasir, Muhammad; Godfrey, Rita E.; Christie, Gabrielle S; Element, Sarah J; Saville, Faye; Hassan, Sedky H.A.; Ahmed, Emad A.; Abufaddan, Nagla Hassan; Daef, Enas; Busby, Stephen J. W.; Browning, Douglas F.

doi:10.1080/21505594.2020.1859852

Cited by 13 publications

(12 citation statements)

References 96 publications

(189 reference statements)

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“…Our results reveal that active rectal screening for CRE and ESBL producers is feasible, with a rate of colonization of 23% and 34%, respectively. Our data were similar to other recent studies, with a rate of CRE colonization reaching 21% [ 4 ] and ESBL colonization reaching 30.6% among hospitalized patients [ 19 ]. Additionally, our data showed that 36% and 27.5% of the positively screened patients had developed subsequent infection with CRE and ESBL producers, respectively.…”

Section: Discussionsupporting

confidence: 92%

“…Infections with MDR organisms or the rising, extensive, drug-resistant Gram-negative bacteria including the carbapenem-resistant Enterobacteriaceae (CRE) and extended-spectrum β-lactamase (ESBL) producers remain the most challenging. This is because of their limited treatment options, their rapid spread among healthcare environments causing outbreaks associated with high mortality rates and their associated prolonged hospital stays with a high economic burden [ 2 , 3 , 4 ]. It is also estimated that by 2030, more than half of Klebsiella pneumoniae and Escherichia coli will be resistant to third-generation cephalosporins [ 5 ].…”

Section: Introductionmentioning

confidence: 99%

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Multimodal Interventions to Prevent and Control Carbapenem-Resistant Enterobacteriaceae and Extended-Spectrum β-Lactamase Producer-Associated Infections at a Tertiary Care Hospital in Egypt

et al. 2021

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The current rise of multidrug-resistant (MDR) Gram-negative Enterobacteriaceae including the extended-spectrum β-lactamase (ESBL)-producing organisms and carbapenem-resistant Enterobacteriaceae (CRE) has been increasingly reported worldwide, posing new challenges to health care facilities. Accordingly, we evaluated the impact of multimodal infection control interventions at one of the major tertiary healthcare settings in Egypt for the aim of combating infections by the respective pathogens. During the 6-month pre-intervention period, the incidence rate of CRE and ESBL-producing clinical cultures were 1.3 and 0.8/1000 patient days, respectively. During the post-intervention period, the incidence of CRE and ESBL producers continued to decrease, reaching 0.5 and 0.28/1000 patient days, respectively. The susceptibility rate to carbapenems among ESBL producers ranged from 91.4% (ertapenem) to 98.3% (imipenem), amikacin (93%), gentamicin (56.9%), and tobramycin (46.6%). CRE showed the highest resistance pattern toward all of the tested β-lactams and aminoglycosides, ranging from 87.3% to 94.5%. Both CRE and ESBL producers showed a high susceptibility rate (greater than 85.5%) to colistin and tigecycline. In conclusion, our findings revealed the effectiveness of implementing multidisciplinary approaches in controlling and treating infections elicited by CRE and ESBL producers.

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Section: Discussionsupporting

confidence: 92%

Section: Introductionmentioning

confidence: 99%

Multimodal Interventions to Prevent and Control Carbapenem-Resistant Enterobacteriaceae and Extended-Spectrum β-Lactamase Producer-Associated Infections at a Tertiary Care Hospital in Egypt

et al. 2021

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“…K. pneumoniae strain KPE16 was isolated from a child, at the outpatient clinic of Assiut University Children’s Hospital in 2016 [ 21 ]. As multidrug resistant K. pneumoniae are a major concern in Egypt [ 6 , 7 , 8 , 9 , 10 , 11 , 12 , 13 ], we examined its susceptibility to various antimicrobials.…”

Section: Resultsmentioning

confidence: 99%

“…Klebsiella pneumoniae strain KPE16 was isolated from a child, presenting with diarrhoea to the outpatients clinic of Assiut University Children’s Hospital in 2016 [ 21 ]. Isolation and original identification were carried out at the Medical Research Center, Faculty of Medicine, Assiut University with growth on MacConkey agar, Eosin Methylene Blue (EMB) agar and IMViC testing, suggesting that KPE16 was an Escherichia coli isolate.…”

Section: Methodsmentioning

confidence: 99%

“…Despite this, the genomes of very few K. pneumoniae strains, isolated in Egypt, have been sequenced and fully characterized [ 19 , 20 ]. During a recent screen in Assiut, Egypt [ 21 ], we isolated a carbapenem resistant K. pneumoniae strain from a child with diarrhoea. Here we present the antimicrobial resistance profile and genome sequence of this strain, K. pneumoniae KPE16, and compare it with other Egyptian isolates, identifying common plasmids, resistance genes and virulence determinants.…”

Section: Introductionmentioning

confidence: 99%

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Antimicrobial Resistance and Comparative Genome Analysis of Klebsiella pneumoniae Strains Isolated in Egypt

et al. 2021

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Klebsiella pneumoniae is an important human pathogen in both developing and industrialised countries that can causes a variety of human infections, such as pneumonia, urinary tract infections and bacteremia. Like many Gram-negative bacteria, it is becoming resistant to many frontline antibiotics, such as carbapenem and cephalosporin antibiotics. In Egypt, K. pneumoniae is increasingly recognised as an emerging pathogen, with high levels of antibiotic resistance. However, few Egyptian K. pneumoniae strains have been sequenced and characterised. Hence, here, we present the genome sequence of a multidrug resistant K. pneumoniae strain, KPE16, which was isolated from a child in Assiut, Egypt. We report that it carries multiple antimicrobial resistance genes, including a blaNDM-1 carbapenemase and extended spectrum β-lactamase genes (i.e., blaSHV-40, blaTEM-1B, blaOXA-9 and blaCTX-M-15). By comparing this strain with other Egyptian isolates, we identified common plasmids, resistance genes and virulence determinants. Our analysis suggests that some of the resistance plasmids that we have identified are circulating in K. pneumoniae strains in Egypt, and are likely a source of antibiotic resistance throughout the world.

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Prevalence, toxin-typing and antimicrobial susceptibility of Clostridium perfringens in sheep with different feeding modes from Gansu and Qinghai provinces, China

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et al. 2022

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Antimicrobial resistance and gene regulation in Enteroaggregative Escherichia coli from Egyptian children with diarrhoea: Similarities and differences

Cited by 13 publications

References 96 publications

Multimodal Interventions to Prevent and Control Carbapenem-Resistant Enterobacteriaceae and Extended-Spectrum β-Lactamase Producer-Associated Infections at a Tertiary Care Hospital in Egypt

Multimodal Interventions to Prevent and Control Carbapenem-Resistant Enterobacteriaceae and Extended-Spectrum β-Lactamase Producer-Associated Infections at a Tertiary Care Hospital in Egypt

Antimicrobial Resistance and Comparative Genome Analysis of Klebsiella pneumoniae Strains Isolated in Egypt

Prevalence, toxin-typing and antimicrobial susceptibility of Clostridium perfringens in sheep with different feeding modes from Gansu and Qinghai provinces, China

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