Antimicrobial Resistance and Extended-Spectrum Beta-Lactamase Production in Enterobacteria Isolated from Free-Living Primates

Zaniolo, Melissa Marchi; Santos, Isabela Carvalho dos; Barbosa, Lidiane Nunes; Pachaly, Evandra Maria Voltarelli; Caetano, Isabel Cristina da Silva; Lopes, Karoline Franciane Cardoso; Marques, Marcelo Aparecido; Mota, Edinalva Almeida; Martins, Lisiane de Almeida; Junior, Ranulfo Piau; Germano, Ricardo de Melo; Pachaly, José Ricardo; Gonçalves, Daniela Dib

doi:10.1089/vbz.2019.2552

Cited by 6 publications

(4 citation statements)

References 10 publications

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“…The patient did not have any recent procedures that involved intravenous or vertebral canal access. Pharyngeal swab cultures did find both E. coli and Enterobacter ; however, these bacteria are common commensals of the gastrointestinal tract in primates 9,10 . These commensals could have spread haematogenously to the vertebrae and led to the osteomyelitis and epidural empyema.…”

Section: Discussionmentioning

confidence: 99%

Spinal epidural empyema in a colobus monkey

Cehrs,

Beltran

2023

Vet Record Case Reports

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A 10‐year‐old, male colobus monkey presented with a kyphotic appearance. Lumbar spinal pain was suspected, and the colobus was started on a course of meloxicam. With no improvement noted, it was sedated for examination. Haematology revealed a neutrophilia. An empirical course of clavulanate potentiated amoxicillin was started. With no improvements seen after a 10‐day course, clinical examination was repeated. Abdominal ultrasound and abdominal radiographs revealed an abdominal effusion near the lumbar vertebrae, and haematology revealed a neutrophilia with toxic neutrophils. An enrofloxacin course was started. Three days later, the colobus presented with acute paraplegia with neurolocalisation to the T3–L3 spinal cord segments. A magnetic resonance imaging of the vertebral column was performed. Magnetic resonance imaging revealed multifocal osteolytic vertebral body lesions, compression fractures of multiple vertebral bodies and ventral spinal cord compression. Differential diagnosis included infectious osteomyelitis with spinal epidural empyema or less likely, neoplasia. Due to the poor prognosis, euthanasia was elected.

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Section: Discussionmentioning

confidence: 99%

Spinal epidural empyema in a colobus monkey

Cehrs,

Beltran

2023

Vet Record Case Reports

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“…ESBL-producing Enterobacteriaceae are critical priority resistant pathogens and were not previously reported in NHP from South America. A recent study found MDR- Enterobacteriaceae in samples taken from free-living black capuchin monkeys ( Sapajus nigritus ) in Brazil, identifying resistant strains to beta-lactam antibiotics, but no ESBL-production was detected ( 39 ). Our ESBL-producing E. coli strains were found to have broad resistome to relevant antibiotics ( Table 1 ).…”

Section: Discussionmentioning

confidence: 99%

Genomic insights into ESBL-producing Escherichia coli isolated from non-human primates in the Peruvian Amazon

Bazalar-Gonzales,

Silvestre-Espejo,

Rodríguez Cueva

et al. 2024

Front. Vet. Sci.

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IntroductionExtended-spectrum beta-lactamase (ESBL)-producing Enterobacteriaceae are on the WHO priority pathogens list because they are associated with high mortality, health-care burden, and antimicrobial resistance (AMR), a serious problem that threatens global public health and should be addressed through the One Health approach. Non-human primates (NHP) have a high risk of acquiring these antibiotic-resistant bacteria due to their close phylogenetic relationship with humans and increased anthropogenic activities in their natural environments. This study aimed to detect and analyze the genomes of ESBL-producing Escherichia coli (ESBL-producing E. coli) in NHP from the Peruvian Amazon.Materials and methodsWe collected a total of 119 fecal samples from semi-captive Saguinus labiatus, Saguinus mystax, and Saimiri boliviensis, and captive Ateles chamek, Cebus unicolor, Lagothrix lagothricha, and Sapajus apella in the Loreto and Ucayali regions, respectively. Subsequently, we isolated and identified E. coli strains by microbiological methods, detected ESBL-producing E. coli through antimicrobial susceptibility tests following CLSI guidelines, and analyzed their genomes using previously described genomic methods.ResultsWe detected that 7.07% (7/99) of E. coli strains: 5.45% (3/55) from Loreto and 9.09% (4/44) from Ucayali, expressed ESBL phenotype. Genomic analysis revealed the presence of high-risk pandemic clones, such as ST10 and ST117, carrying a broad resistome to relevant antibiotics, including three blaCTX-M variants: blaCTX-M-15, blaCTX-M-55, and blaCTX-M-65. Phylogenomic analysis confirmed the clonal relatedness of high-risk lineages circulating at the human-NHP interface. Additionally, two ESBL-producing E. coli strains were identified as EPEC (eae) and ExPEC according to their virulence profiles, and one more presented a hypermucoviscous phenotype.DiscussionWe report the detection and genomic analysis of seven ESBL-producing E. coli strains carrying broad resistome and virulence factors in NHP from two regions of the Peruvian Amazon. Some of these strains are closely related to high-risk pandemic lineages previously reported in humans and domestic animals, highlighting the negative impact of anthropogenic activities on Amazonian wildlife. To our knowledge, this is the first documentation of ESBL-producing E. coli in NHP from the Amazon, underscoring the importance of adopting the One Health approach to AMR surveillance and minimizing the potential transmission risk of antibiotic-resistant bacteria at the human-NHP interface.

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“…Tomahawk traps were distributed inside the park and the baits to attract S. nigritus (Goldfuss, 1809) were composed of elements from the animals' usual diet, such as fruits and eggs (Zaniolo et al., 2020).…”

Section: Methodsmentioning

confidence: 99%

Urban capuchin monkeys Sapajus nigritus (Goldfuss, 1809) (Primates, Cebidae) as environmental bioindicators of leishmaniasis

Lopes

Delai

Zaniolo

et al. 2021

Transbounding Emerging Dis

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The aim of this study was to investigate the presence of Leishmania sp. DNA and anti-Leishmania spp. antibodies in free-ranging Sapajus nigritus from an urban forest located in a city in the North Central region of the state of Paraná. For the indirect diagnosis, the direct agglutination test was used with promastigote forms of Leishmania (V.) braziliensis, where it was possible to detect the agglutination reaction in 53.33% of the S. nigritus blood samples. For direct diagnosis, the samples were submitted to real-time quantitative polymerase chain reaction, which confirmed the presence of Leishmania spp. DNA in 26.66% of the tested samples. It reinforces the importance of considering the concept of One Health in the face of diseases with high prevalence, such as leishmaniasis and the need for health education measures. This result shows that the animals in the present study have a role as environmental bioindicators for leishmaniasis.

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Antimicrobial Resistance and Extended-Spectrum Beta-Lactamase Production in Enterobacteria Isolated from Free-Living Primates

Cited by 6 publications

References 10 publications

Spinal epidural empyema in a colobus monkey

Spinal epidural empyema in a colobus monkey

Genomic insights into ESBL-producing Escherichia coli isolated from non-human primates in the Peruvian Amazon

Urban capuchin monkeys Sapajus nigritus (Goldfuss, 1809) (Primates, Cebidae) as environmental bioindicators of leishmaniasis

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