Antimicrobial properties of silver nanoparticles against biofilm formation by Pseudomonas aeruginosa on archaeological textiles

Abstract

The aims of this work were to: (i) microscopically analyse the pre-and post-Columbian archaeological textiles using Scanning Electron Microscopy with Energy Dispersive X-Ray Analysis (SEM-EDX); (ii) microbiologically analyse the archaeological textiles (from the Southern Andean Area, La Plata Museum); (iii) determine the ability of Pseudomonas sp. isolates from archaeological textiles to biofilm formation by SEM; (iv) assess the anti-biofilm properties of AgNPs protecting cotton against Pseudomonas sp. Results showed the presence of bacteria with proteolytic and lipolytic activities on archaeological textiles, including Clostridium sp. and Pseudomonas aeruginosa. Two nucleotide sequences of 16S ribosomal RNA gene of P. aeruginosa strains were deposited in GeneBank NCBI database with accession numbers: KP842564 (strain 1) and KP842565 (strain 2). Those strains exhibited different morphological and growth characteristics: strain 1 with ability to form biofilms on archaeological textiles was rod-shaped, produced bluish-green pigment, and smaller than strain 2; and strain 2 was pleomorphic and produced brown pigment. The use of silver nanoparticles (90 ppm, φ 10-80 nm) allowed to protecting textiles against P. aeruginosa growth by 63%-97%, depending on the strain and exposition time.

SYNTHESIS AND CHARACTERIZATION OF SILVER NANOPARTICLES MEDIATED BY THE Mentha piperita LEAVES EXTRACT AND EXPLORATION OF ITS ANTIMICROBIAL ACTIVITIES

“…According to the literature, AgNPs effectively prevent Pseudomonas aeruginosa from forming biofilms, however, it only occurs when nanoparticles in liquid culture come into frequent contact with the bacterial cells [48]. Gram-negative bacteria are typically more resilient than Gram-positive bacteria, making them more challenging to deal with because their outer membrane covers the peptidoglycan cell wall [49]. The permeability and respiratory activity of the bacterial cells are unstable when silver nanoparticles are connected to the cell membrane's surface.…”

A light-switch for efficient decolorization of azo dye wastewater based on bacteria–semiconductor interaction

“…S3a-c, † S. oneidensis MR-1/CdS still had good cell viability and intact cell morphology after the decolorization reaction. Considering the possible damage Environmental Science: Nano Paper of h + and ˙OH to bacterial cells, 5,44 the S. oneidensis MR-1/ CdS system must have a self-protection and regulation mechanism. The results in 3.5 showed that bioelectrons could serve as h + scavengers to ensure efficient h + quenching and photoelectron separation.…”