Antimicrobial properties of probiotic strains isolated from breast-fed infants

Rodrı́guez, E.; Arqués, Juan Luís; Rodríguez, Roberto Sánchez; Peirotén, Ángela; Landete, José María; Médina, Margarita

doi:10.1016/j.jff.2012.02.015

Cited by 64 publications

(36 citation statements)

References 60 publications

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“…Lactobacilli act competitively to inhibit pathogen colonization and consequently prevent infection . Lactobacilli exhibit antimicrobial activity against a broad range of pathogenic microorganisms, including E. coli , S. typhimurium , and S. aureus .…”

Section: Discussionmentioning

confidence: 99%

Assessment of probiotic potential and anticancer activity of newly isolated vaginal bacterium Lactobacillus plantarum 5BL

Nami

Abdullah

Haghshenas

et al. 2014

Microbiology and Immunology

100

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Numerous bacteria in and on its external parts protect the human body from harmful threats. This study aimed to investigate the potential beneficial effects of the vaginal ecosystem microbiota. A type of bacteria was isolated from vaginal secretions of adolescent and young adult women, cultured on an appropriate specific culture medium, and then molecularly identified through 16S rDNA gene sequencing. Results of 16S rDNA sequencing revealed that the isolate belongs to the Lactobacillus plantarum species. The isolated strain exhibited probiotic properties such as low pH and high bile salt concentration tolerance, antibiotic susceptibility and antimicrobial activity against some pathogenic bacteria. The anticancer effects of the strain on human cancer cell lines (cervical, HeLa; gastric, AGS; colon, HT-29; breast, MCF-7) and on a human normal cell line (human umbilical vein endothelial cells [HUVEC]) were investigated. Toxic side effects were assessed by studying apoptosis in the treated cells. The strain exhibited desirable probiotic properties and remarkable anticancer activity against the tested human cancer cell lines (P 0.05) with no significant cytotoxic effects on HUVEC normal cells (P 0.05). Overall, the isolated strain showed favorable potential as a bioactive therapeutic agent. Therefore, this strain should be subjected to the other required tests to prove its suitability for clinical therapeutic application.Key words anticancer, apoptosis, microbial biotherapy.The human vagina is colonized by different microbes, over 50 microbial species promoting female health by protecting against pathogens, modulating immunity, preventing infection and maintaining the pH below 4.7 (1-3). A variety of Lactobacillus species have been identified as the predominant biota in the vagina (4): over 70% of all bacteria sampled from the vaginas of healthy women are lactobacilli (5). The ability of lactobacilli to adhere to epithelial cells and colonize the vagina is attributable to factors such as hormonal factors, vaginal pH and glycogen content (6).The protective effects of normal vaginal flora, particularly lactobacilli, are increasingly being accepted (7). Lactobacilli adjust the vaginal microbiota through various mechanisms, including auto-aggregation, coaggregation with pathogenic microorganisms, adhesion to epithelial cells, and production of various antimicrobial substances, such as lactic acid, bacteriocins,

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Section: Discussionmentioning

confidence: 99%

Assessment of probiotic potential and anticancer activity of newly isolated vaginal bacterium Lactobacillus plantarum 5BL

Nami

Abdullah

Haghshenas

et al. 2014

Microbiology and Immunology

100

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“…The high ability of adhesion of the four bifidobacteria to cutaneous cells (keratinocytes) obtained in our study is quite unexpected considering their intestinal origin, but is a desirable property because it allows the formation of a barrier that prevents the colonization by possible pathogens (Rodríguez et al . ; Saad et al . ).…”

Section: Resultsmentioning

confidence: 99%

In vitro evaluation of antagonism, modulation of cytokines and extracellular matrix proteins by Bifidobacterium strains

Silva

Nicoli

et al. 2018

Lett Appl Microbiol

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The skin is the first line of defence against invasive micro-organisms, and its local microbiota provides additional protective functions based on antagonism against pathogenic micro-organisms and immunomodulation. Based on in vitro assays using Bifidobacterium spp. we demonstrated the antagonistic potential, as well as capacity in stimulating the production of cytokines and proteins of the extracellular matrix that these bacteria may exert on skin cells. This positive influence suggests the use of a consortium of these bifidobacteria in a topical product for dermatological treatments.

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“…Numerous studies report on the inhibition of a wide range of pathogenic microorganisms, by bifidobacteria, both in vitro and in vivo (Yildirim et al, 1999;De Vuyst et al, 2004;Servin, 2004;Makras and De Vuyst, 2006;Cheikhyoussef et al, 2008;Kondepudi et al, 2012;Rodriguez et al, 2012). Antimicrobial activity of bifidobacteria was first noted by Tissier (1900), who described various types of antagonistic effects of B. bifidum against Escherichia coli (Tissier, 1990).…”

Section: Discussionmentioning

confidence: 99%

“…Numerous studies showed that bifidobacteria have antimicrobial activity against several pathogenic microbial groups. There is also increasing interest in the research of antimicrobial peptides such as bacteriocins and bacteriocin-like compounds produced by bifidobacteria (Yildirim et al, 1999;De Vuyst et al, 2004;Servin, 2004;Makras and De Vuyst, 2006;Cheikhyoussef et al, 2008;Kondepudi et al, 2012;Rodriguez et al, 2012). However, only a very small number of antimicrobial compounds produced by bifidobacteria have been purified and characterized, despite their apparent benefits to human health.…”

mentioning

confidence: 99%

Probiotics with Antimicrobial Activity against Multidrug Resistant Pseudomonas aeruginosa and Acinetobacter baumannii

Lee¹,

Kim²,

Kang³

et al. 2013

The Korean Journal of Microbiology

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Pseudomonas aeruginosa and Acinetobacter baumannii are significant opportunistic pathogens in hospitals and are resistant to most antibiotics. Multidrug-resistant P. aeruginosa (MDRPA) and A. baumannii (MDRAB) cause severe human nosocomial infections and are more difficult to treat than methicillin-resistant Staphylococcus aureus (MRSA). Bifidobacteria are among of the most beneficial probiotics and have been widely studied for their antimicrobial activities. The present study explored the antimicrobial activity of Bifidobacterium sp. isolated from healthy Koreans against MDRPA and MDRAB. The antimicrobial activity of the isolates against MDRPA and MDRAB, which are resistant to ciprofloxacin, tobramycin, gentamicin, meropenem, and ceftazidime, was determined by modified broth microdilution methods using absorbance. Among all tested bifidobacteria isolates (nine B. adolescentis, three B. longum, and two B. pseudocatenulatum), the culture supernatant of B. pseudocatenulatum SPM1309 showed a strong growth inhibitory effect against MDRPA and MDRAB. No change in the turbidity of the mixture was observed during incubation, and its inhibitory effect occurred through bacteriostastic action. Moreover, the antibacterial activity was observed in the fraction with molecular weights <10 kDa of bifidobacteria culture supernatant, and the active fraction was heat-stable because it maintained its activity when heated at 70℃ for 10 min. The results suggest that this Bifidobacterium strain could have potential applications for alternative therapy in MDRPA and MDRAB infections.

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Antimicrobial properties of probiotic strains isolated from breast-fed infants

Cited by 64 publications

References 60 publications

Assessment of probiotic potential and anticancer activity of newly isolated vaginal bacterium Lactobacillus plantarum 5BL

Assessment of probiotic potential and anticancer activity of newly isolated vaginal bacterium Lactobacillus plantarum 5BL

In vitro evaluation of antagonism, modulation of cytokines and extracellular matrix proteins by Bifidobacterium strains

Probiotics with Antimicrobial Activity against Multidrug Resistant Pseudomonas aeruginosa and Acinetobacter baumannii

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