Antimicrobial properties and dental pulp stem cell cytotoxicity using carboxymethyl cellulose-silver nanoparticles deposited on titanium plates

Abstract

Objective: To evaluate the antimicrobial properties and dental pulp stem cells (DPSCs) cytotoxicity of synthesized carboxymethyl cellulose-silver nanoparticles impregnated on titanium plates. Material and methods: The antibacterial effect of silver nanoparticles in a carboxymethyl cellulose matrix impregnated on titanium plates (Ti-AgNPs) in three concentrations: 16%, 50% and 100% was determined by adding these to bacterial cultures of Streptococcus mutans and Porphyromonas gingivalis. The Ti-AgNPs cytotoxicity on DPSCs was determined using a fluorimetric cytotoxicity assay with 0.12% chlorhexidine as a positive control. Results: Silver nanoparticles in all concentrations were antimicrobial, with concentrations of 50% and 100% being more cytotoxic with 4% cell viability. Silver nanoparticles 16% had a cell viability of 95%, being less cytotoxic than 0.12% chlorhexidine. Conclusions: Silver nanoparticles are a promising structure because of their antimicrobial properties. These have high cell viability at a concentration of 16%, and are less toxic than chlorhexidine.

Influence of Polysaccharides’ Molecular Structure on the Antibacterial Activity and Cytotoxicity of Green Synthesized Composites Based on Silver Nanoparticles and Carboxymethyl-Cellulose

“…We previously reported on the ability of green synthesized CMC-AgNPs composites to inhibit the proliferation of Gram-positive and negative bacteria, such as Streptococcus mutans and Porphyromonas gingivalis, respectively, with a suitable cytotoxicity [20]. Nonetheless, currently, experimental evidence regarding the role of molecular structure of polysaccharides as CMC on the antibacterial activity and cytotoxicity of AgNPs-based composites does not exists.…”

Hydroxyapatite decreases cytotoxicity of a glass ionomer cement by calcium fluoride uptake in vitro

“…After 24 hours of incubation, the medium was removed, and the cells were washed 3 times with phosphate-buffered saline (PBS). The cytotoxicity assay was performed as described by Larsson and Nygren (15, 16). Briefly, fluorescein diacetate (FDA; Sigma-Aldrich) was dissolved in dimethyl sulfoxide (DMSO; Sigma-Aldrich) and kept frozen at -20°C as a stock solution (10 mg/mL).…”

Elucidation of the Anodization and Silver Incorporation Impact on the Surface Properties of AA1050 Aluminum Alloy

“…Further, the commercial availability of the electrochemical silver incorporation into AAO layers for antibacterial packaging products was demonstrated by Ikeda et al 57 These apparent bactericide properties have inspired the recent intensive research activities regarding the preparation of silver containing composite films. Thus, silver presence in biopolymers, [58][59][60][61][62] graphene oxide, 63 zeolites and zirconium phosphate silicate 64 is already reported.…”