Antimicrobial properties and acute toxicity evaluation of Pycnanthus angolensis stem bark

Chukwudozie, Kingsley Ikechukwu; Ezeonu, Ifeoma M.

doi:10.1016/j.sciaf.2022.e01185

Cited by 5 publications

(5 citation statements)

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“…The potency was concentration and dose-dependent. This current study nding is consistent with a similar study by Chukwudozie and Ezeonu (21). Chukwudozie and Ezeonu (21), reported that the stem bark of P. angolensis showed higher inhibition when tested against Grampositive and negative bacteria.…”

Section: Phytoconstituents Screenedsupporting

confidence: 93%

“…This current study nding is consistent with a similar study by Chukwudozie and Ezeonu (21). Chukwudozie and Ezeonu (21), reported that the stem bark of P. angolensis showed higher inhibition when tested against Grampositive and negative bacteria. In those studies, the ethanol extracts of the plant extracts were more susceptible than the aqueous extracts when used against the selected microorganisms ( 21).…”

Section: Phytoconstituents Screenedsupporting

confidence: 93%

“…It is also historically used to treat insomnia, although the mechanism underlying this has yet to be fully understood (17). Despite the prevalent use of P. angolensis and C. sanguinolenta since time immemorial, much knowledge has yet to be found in the literature evaluating their antimicrobial and antioxidative properties (11,(18)(19)(20)(21). The current study, therefore, seeks to inoculum suspension.…”

Section: Introductionmentioning

confidence: 99%

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Antimicrobial activities and total phenolic contents of the sap of Pycnanthus angolensis and root of Cryptolepis sanguinolenta

Adu-Amankwaah

Sam

Asare

et al. 2023

Preprint

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The death of many people in tropical countries can be attributed to microbial infection, probably, because synthetic antibiotics are failing in the treatment of these microbial infections, attributed to the ability of the microorganisms to mutate and adapt to harsh conditions. This study evaluated, in vitro, the antimicrobial activities, antioxidant potentials, and the total phenolic contents of aqueous and ethanol extracts of the root of Cryptolepis sanguinolenta (Lindl.) and the crude sap of Pycnanthus angolensis (Welw) using selected standard bacteria strains (S. aureus (ATCC 25923), S. saprophyticus (ATCC 15305), E. coli (ATCC 25922), S. typhi (ATCC 19430), P. aeruginosa (ATCC 27853), and P. mirabilis (ATCC 49565). The modified agar well diffusion method was used to evaluate the antimicrobial activities of the plant extracts. Chloramphenicol and tetracycline were used as positive controls. The extracts were also screened for specific phytochemicals and using the Folin Ciocalteu reagent test, their total phenolic contents were determined. The phytochemical screening revealed the presence of alkaloids, cardiac glycosides, and saponins in both Cryptolepis sanguinolenta and Pycnanthus angolensis. For the antimicrobial activities, all the test bacteria were susceptible to the crude sap of Pycnanthus angolensis except P. mirabilis. In the case of the Cryptolepis sanguinolenta, only S. aureus was susceptible to both aqueous and ethanol extracts. The total phenolic content, expressed in g/100g GAE, recorded values of 55.427 ± 4.248 for the crude sap of Pycnanthus angolensis, and 11.642 ± 4.248 and 26.888 ± 4.248 for the aqueous and ethanol extracts of Cryptolepis sanguinolenta, respectively. It was concluded that Cryptolepis sanguinolenta and Pycnanthus angolensis are excellent candidates for further development of antimicrobial agents in the fight against microbial infections given the pressing need for novel efficacious agents.

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Section: Phytoconstituents Screenedsupporting

confidence: 93%

Section: Phytoconstituents Screenedsupporting

confidence: 93%

Section: Introductionmentioning

confidence: 99%

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Antimicrobial activities and total phenolic contents of the sap of Pycnanthus angolensis and root of Cryptolepis sanguinolenta

Adu-Amankwaah

Sam

Asare

et al. 2023

Preprint

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“…Este resultado corrobora com os dados apresentados, onde detectouse a presença de flavonoides no extrato. A presença de flavonoides, taninos e saponinas são indicativos de uma possível atividade antimicrobiana, sendo que a presença destas classes de bioativos, em diversas ocasiões já foi relacionada a inibição de microrganismos (BAKY et al, 2021;DE ARAÚJO et al, 2021;CHUKWUDOZIE;EZEONU, 2021;KISA et al, 2022). Logo, conforme esperando, ambos os extratos apresentaram atividade antimicrobiana (tabela 2), e o efeito da combinação foi avaliado.…”

Section: Determinação Do íNdice Da Concentração Inibitória Fracionári...unclassified

TESTE DE SINERGISMO DA ATIVIDADE ANTIMICROBIANA DOS EXTRATOS DE Eugenia uniflora E PRÓPOLIS POR MÉTODO DE CHECKERBOARD

Dalmagro

Donadel

Pinc

et al. 2022

REMS

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Introdução: A crescente resistência bacteriana aos antibióticos tornou-se uma ameaça à população. Devido ao avanço da fitoterapia nos últimos anos, estudos sobre a ação antimicrobiana de diferentes bioativos têm sido incentivados cada vez mais. De acordo com a literatura, Eugenia uniflora apresenta composição química complexa e seus compostos possuem atividade terapêutica anti-inflamatória, hipotensora, hipoglicemiante, antipirética e antibacteriana. No própolis, foram identificados cerca de 300 compostos relacionados a atividades farmacológicas como anti-inflamatória, antioxidante, antifúngica, hepatoprotetora e antibacteriana. Objetivo: Portanto, o trabalho teve como objetivo avaliar a atividade antimicrobiana individual e combinada dos extratos de E. uniflora e Própolis por checkerboard. Metodologia: Os extratos foram testados em triplicata, frente a Candida albicans, Escherichia coli e Staphylococcus aureus. A incubação foi realizada por 24 horas a 35 ± 2 ºC para bactérias, e 48 horas a 25 ± 2 ºC para o fungo. A avaliação dos resultados bacterianos foi realizada empregando cloreto de trifeniltetrazólio a 2% e a multiplicação fúngica pela turvação do meio. Resultados: Foram encontrados os valores de 62,50 e 7,81 mg/mL para C. albicans, E. coli 7,81 e 3,90 mg/ mL e S. aureus 7,81 e 7,81 mg/mL, para própolis e E. uniflora, respectivamente, quando combinados. Ao calcular o índice de concentração inibitória fracional, observou-se que os valores se compreenderam entre 0,74 e 1,06, indicando que não ocorreu interação entre os extratos avaliados. Conclusão: Assim, apesar da atividade antimicrobiana já comprovada para os extratos de E. uniflora e própolis, a combinação dos extratos não apresentou efeito sinérgico nas condições e metodologia avaliada.

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“…7 The sap possesses anti-hemorrhagic properties and the bark is a poison antidote and treatment of leprosy, infertility, anemia, gonorrhea and malaria. 8 The potential antioxidants from plants such as tannin, terpenoid, saponin, polar flavonoid, phenol, non-polar flavonoid, ascorbic acid, and others possess the ability to scavenge free radicals. 9 Their wide range of effects on the biological system has put forward many experimental works.…”

Section: Introductionmentioning

confidence: 99%

Phytochemical Analysis and Antioxidant Activity of Stem Bark Extract and Fractions of Pycnanthus angolensis Myristicaceae (Welw) Warb

2022

TJNPR

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Pycnanthus angolensis is used extensively in African traditional medicine for treating stressrelated ailments. Antioxidants play an essential role in scavenging and inhibiting free radicals and hence protecting human beings against infection and related degenerative diseases. Modern research is directed toward natural antioxidants from medicinal plants due to their safety as therapeutic agents. The present study is aimed at evaluating the phytochemical constituents and antioxidant activity of the stem bark extract and fractions of Pycnanthus angolensis. The phytochemical investigation was performed by using standard protocols. The extract and fractions were investigated for the antioxidant activity with ascorbic acid as the reference standard by using the 2,2-diphenyl1-1-picrylhydrazyl (DPPH) in-vitro model. Phytochemical analysis of the methanol extract and solvent fractions of P. angolensis stem bark showed the presence of alkaloids, flavonoids, tannin, saponin, terpenoids and steroids. The crude methanol extract (ME), hexane fraction (HF), ethyl acetate fraction (EF) and methanol fraction (MF) showed in vitro antioxidant IC50 of 9.44 ± 1.40 µg/mL, 5.47 ± 0.18 µg/mL, 3.86 ± 1.60 µg/mL, 1.09 ± 2.27 µg/mL, 6.89 ± 3.96 µg/mL and 31.33 ± 2.75 µg/mL respectively. The MF, with an IC50 and ARP of 1.09 µg/ml and 91.7% respectively, appeared to provide a starting point for the discovery of potent antioxidant lead compounds from Pycnanthus angolensis.

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Antimicrobial properties and acute toxicity evaluation of Pycnanthus angolensis stem bark

Cited by 5 publications

References 11 publications

Antimicrobial activities and total phenolic contents of the sap of Pycnanthus angolensis and root of Cryptolepis sanguinolenta

Antimicrobial activities and total phenolic contents of the sap of Pycnanthus angolensis and root of Cryptolepis sanguinolenta

TESTE DE SINERGISMO DA ATIVIDADE ANTIMICROBIANA DOS EXTRATOS DE Eugenia uniflora E PRÓPOLIS POR MÉTODO DE CHECKERBOARD

Phytochemical Analysis and Antioxidant Activity of Stem Bark Extract and Fractions of Pycnanthus angolensis Myristicaceae (Welw) Warb

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