Antimicrobial Pressure of Ciprofloxacin and Gentamicin on Biofilm Development by an Endoscope-Isolated <i>Pseudomonas aeruginosa</i>

Machado, Idalina; Graça, Joana; Lopes, Helena; Lopes, Susana Patrícia

doi:10.5402/2013/178646

Cited by 17 publications

(11 citation statements)

References 42 publications

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“…It is one of the main causes of hospital-acquired infections (Machado et al, 2013). Effective antibiotics are limited because infections by P. aeruginosa are hard to defy, as it is virulent, acquires antibiotic resistance and resists disinfectants (Higgins et al, 2001;Zavascki et al, 2005).…”

Section: Introductionmentioning

confidence: 99%

“…Biofilm resistance mechanisms include not only the reaction-diffusion limitation of antimicrobial access to the biofilm-entrapped bacteria (Gilbert et al, 2003), but also the expression of spatially heterogeneous, less susceptible phenotypes, caused either by growth as a biofilm per se, or through the expression of high cell density, or starvation phenotypes. The use of certain active substances in biocides in various settings may contribute to the increased occurrence of antibiotic resistant bacteria (Machado et al, 2013).…”

Section: Introductionmentioning

confidence: 99%

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Effect of Disinfectants on Antibiotics Susceptibility of Pseudomonas aeruginosa

Al-Jailawi¹,

Ameen²,

Al-Jeboori³

2013

Journal of Applied Biotechnology

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Disinfectants are widely used to get rid of microorganisms whether in hospitals, health centers or for normal domestic use. Some suggested that when disinfectants are incorrectly diluted the disinfectant might promote the growth of antibiotic-resistant bacteria, therefore, in this study pathogenic bacterium (Pseudomonas aeruginosa), isolated from patient with urinary tract infection, treated with two locally popular disinfectants (Claradone and Sarttol). Results showed that the bacterial growth was affected by both disinfectants. The lowest concentration of Claradone that inhibit the growth of this bacterium is considered as the minimum inhibitory concentration (MIC), this was 30%, while the lowest effecting concentration of Sarttol was 3%. A number of survival colonies after treated with high concentration of Calarodone and Sarttol were investigated for their susceptibility to antibiotics, using standard disc diffusion method. Results indicated that these colonies of P. aeruginosa resisted antibiotics they were sensitive to before treatment. So it can be concluded that using Claradone and sarttol can make the pathogenic bacterium (P. aeruginosa) resist some antibiotics.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Effect of Disinfectants on Antibiotics Susceptibility of Pseudomonas aeruginosa

Al-Jailawi¹,

Ameen²,

Al-Jeboori³

2013

Journal of Applied Biotechnology

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“…It has been reported that most of the antibacterial agents are unable to penetrate in biofilm consortia, that leads to serious therapeutic problems in control of various infections [3,4]. Biofilms act a reservoir of J Microbiol Infect Dis www.jmidonline.org Vol 10, No 1, March 2020 pathogenic bacteria that can detach, resume their planktonic state, and contaminate new surfaces and patients [5]. P. aeruginosa is one of the leading colonizers in clinical setups including indwelling biomedical catheters and implants [1].…”

mentioning

confidence: 99%

“…P. aeruginosa is one of the leading colonizers in clinical setups including indwelling biomedical catheters and implants [1]. Most of the times these pathogens adopt a biofilm mode of life to nullify the toxic effect of antibiotics [3][4][5]. According to research reports, biofilm communities of P. aeruginosa consist of heterogeneous population, e.g.…”

mentioning

confidence: 99%

Role of Phenotypic Switching in Stability and Persistence of Pseudomonas aeruginosa Biofilms

Mirani

Urooj

Zeesahn

et al. 2020

Journal of Microbiology and Infectious Diseases

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Objectives: Objective: This study was designed to explore the role of different phenotypes of P. aeruginosa in the development, stability and persistence of biofilm. Methods: A total of seventeen (17) waterborne biofilm producing strains of P. aeruginosa were studied. These isolates were identified on the basis of typical phenotypic characters and the tube method was used for the study of biofilms. Population and phenotypic variance were studied by the drop plate method. The hydrophobicity of strains was evaluated by the bacterial adhesion to apolar solvent test. Results: Study showed that the subject isolates of P. aeruginosa adopted a biofilm life style after 36 h of incubation at 35 °C. After 24 h the adhesion started, but it was reversible and easily dispersed by simple washing. However, after 36 h the irreversible adhesion was noticed. The biofilm consortia harbor three different phenotypes: i. wild types, showed typical P. aeruginosa characters on Cetrimide agar; ii. Slow growers, showed poor pigmentation and take >36 h for colony development, and iii. Small colony variants (SCVs) are metabolically inactive and producing pinpointed non pigmented colonies. Comparative analysis showed that these phenotypes i.e. SCVs were highly hydrophobic and persistent in biofilm consortia due to the production of excessive amounts of exopolysaccharides. Conclusions: This study showed that phenotypic heterogeneity is a characteristic feature of P. aeruginosa biofilms and all of these phenotypes have a major role in stability and persistence of biofilm consortia.

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“…aeruginosa is an opportunistic pathogen and responsible for hospital-acquired infections (1,2). P. areuginosa is a virulent, antibiotic resistant and sometime resists disinfectants.…”

Section: Introductionmentioning

confidence: 99%

Comparison between reduced susceptibility to disinfectants and multidrug resistance among hospital isolates of pseudomonas aeruginosa and staphylococcus aureus in bangladesh

Islam¹,

Saha²,

Sultana³

et al. 2017

Bagcilar Medical Bulletin

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Disinfectants have been used largely in hospitals, health care centers and different pharmaceuticals for the removal of microorganisms. It is evident that microorganisms are showing reduced sensitivity against many disinfectants or their minimum inhibitory concentration (MIC) is increasing day by day due to improper use. The aim of this study was to compare the reduced susceptibility to disinfectants and antibiotics of 25 hospital isolates of Pseudomonas aeruginosa and 40 hospital isolates of Staphylococcus aureus isolated from 5 different hospitals at Noakhali region of Bangladesh. Susceptibility of the selected isolates to two disinfectants (savlon and herpic) and ten separate antimicrobial agents for both P. aeruginosa and S. aureus were investigated and compared. Multidrug resistant pattern of all the hospital isolates were determined by agar diffusion method and MIC of the disinfectants were determined by the serial dilution method. All the hospital isolates of P. aeruginosa and S. aureus were multidrug resistant. No severe evident resistance to disinfectants was seen among the 25 isolates of P. aeruginosa and 40 isolates of S. aureus. Interestingly, satisfactory MIC of savlon for 25 isolates of P. aeruginosa and 40 isolates of S. aureus reached at 0.5% to 0.7% (v/v) solution whereas satisfactory MIC of herpic reached at 2% to 2.5% (v/v) solution for all hospital isolates but four isolates of S. aureus showed MIC against herpic at 1.75% (v/v) solution. No sign of co-resistant of disinfectant and antibiotics were found. So, it can be concluded that disinfectants (savlon and herpic) can't be responsible for P. aeruginosa and S. aureus to become multidrug resistant, when the semi inhibitory dilution of these disinfectants are used. Keywords: disinfectants, multidrug resistant, P. aeruginosa, S. aureus ÖZET Bangladeş'teki hastanelerde toplanan Pseudomonas aeruginosa ve Staphylococcus aureus izolatlarında bulunan dezenfektanlara karşı azaltılmış duyarlılık ve çoklu ilaca direnç gelişimi arasında karşılaştırma Amaç: Mikroorganizmaların giderilmesini sağlamak amacıyla hastaneler ve sağlık tesislerinde dezenfektanlar ve çeşitli tür ilaçlar kullanılmaktadır. Bilindiği gibi uygunsuz kullanımdan dolayı mikroorganizmaların dezenfektanlara karşı gösterdiği duyarlılık azalıyor ya da minimum engelleyici konsantrasyonu (MEK) günbegün artıyor. Çalışmamız, Bangladeş'in Noakhali bölgesinde bulunan 5 hastaneden alınan 25 tane Pseudomonas aeruginosa ve 40 tane Staphylococcus aureus hastane izolatlarında, dezenfektanlara ve antibiyotiklere karşı gelişen azaltılmış direnç derecelerini karşılaştırmayı amaçladı. Seçilmiş olan P. aeruginosa ve S. aureus izolatlarının iki dezenfektan (Savlon ve Harpic) ve on tane farklı antimikrobiyal ajana karşı gösterdiği duyarlılıklar araştırılıp karşılaştırıldı. Bütün hastane izolatlarının çoklu ilaca direnç kalıpları, agar diffüzyon yöntemi kullanarak saptanıp dezenfektanların MEK'leri seri seyreltme yöntemiyle bulundu. Hem P. aeruginosa hem de S. aureus'un bütün hastane izolatları çoklu...

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Antimicrobial Pressure of Ciprofloxacin and Gentamicin on Biofilm Development by an Endoscope-Isolated Pseudomonas aeruginosa

Cited by 17 publications

References 42 publications

Effect of Disinfectants on Antibiotics Susceptibility of Pseudomonas aeruginosa

Effect of Disinfectants on Antibiotics Susceptibility of Pseudomonas aeruginosa

Role of Phenotypic Switching in Stability and Persistence of Pseudomonas aeruginosa Biofilms

Comparison between reduced susceptibility to disinfectants and multidrug resistance among hospital isolates of pseudomonas aeruginosa and staphylococcus aureus in bangladesh

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