Antimicrobial Potential of Crude Extract of Curvularia lunata, an Endophytic Fungi Isolated from Cymbopogon caesius

Abstract

Cymbopogon caesius grass is an aromatic tall grass species which grows in waste field and along the edges of crop fields in Karnataka. From this grass fungal endophytes were isolated on PDA medium and identified as Curvularia lunata. The fungus was cultured on Potato Dextrose broth for production of fungal metabolites. Ethyl acetate extract of the C. lunata was obtained by liquid-liquid partition of broth of endophyte and evaporation. Dried crude extract was tested for antimicrobial activity by agar well diffusion method. The extract showed antimicrobial nature against all the test pathogens. Crude extract of C. lunata is highly active against Staphylococcus aureus with the inhibition percentage of 92 and Candida albicans with 81 percent inhibition.

Bioactive secondary metabolites from new endophytic fungus Curvularia. sp isolated from Rauwolfia macrophylla

“…indicating their importance as talented and major sources of bioactive molecules [9, 44]. However, the fungal metabolites have increased the interest in biomolecules activity studies [10].…”

“…Endophytic fungi Curvularia sp. including Curvularia trifolii [9] and Curvularia lunata [10] provide a broad variety of bioactive secondary metabolites including polyketides and steroids. The genus Curvularia includs more than 40 species of saprophytes, endophytes and pathogens [11].…”

Hr-LCMS Based Mychochemical Profiling of Endophytic Xylaria Adscendens (Fr.) Fr., in Wendlandia Thyrsoidea (Roth) Steud. Of Central Western Ghats

“…The pure culture of X. adscendens was inoculated in 1000ml Erlenmeyer flask containing 500ml of Potato dextrose broth on a rotary shaker for 15-22 days at 25° by fermentation method (Fig. 3.C) The culture filtrate was filtered and extracted with the same volume of Ethyl acetate twice and the extract was rotary evaporator dried for further analysis (Avinash et al, 2015). The ethyl acetate crude extract was analysed by HR-LCMS.…”

Evaluation of Antiviral, Antibacterial and Antiproliferative Activities of the Endophytic Fungus Curvularia papendorfii, and Isolation of a New Polyhydroxyacid

“…crude extracts have promising antibacterial activities. The extract of C. lunata showed antibacterial activities against S. aureus and S. typhi [17], and the ethyl acetate crude extract of C. tuberculata has shown antibacterial activities against S. aureus, E. coli and P. aeruginosa [39]. Moderate activities of extract of Curvularia B34 against Bacillus subtilis, Listeria monocytogenes and Salmonella bacteria have been observed [37].…”

“…Many species are known to be plant pathogens [16]. Some researchers have demonstrated that crude extracts of some Curvularia species have interesting properties including antimicrobial, antioxidant, phytotoxicity and leishmanicidal activities [12,[17][18][19].…”