Antimicrobial polyketide furanoterpenoids from seaweed-associated heterotrophic bacterium Bacillus subtilis MTCC 10403

Chakraborty, Kajal; Thilakan, Bini; Raola, Vamshi Krishna

doi:10.1016/j.phytochem.2017.06.019

Cited by 33 publications

(14 citation statements)

References 45 publications

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“…New compounds 152-200 were reported from the Actinobacteria genera Nocardiopsis, Micromonospora, Saccharomonospora, Rubrobacter, Nesterenkonia, Microbacterium, Actinoalloteichus and Saccharopolyspora. New compounds from the Firmicutes genera Bacillus, Geobacillus and Thermoactinomyces include 201-216, [98][99][100][101][102][103][104][105][106] while new NPs from the Proteobacteria genera Pseudoalteromonas, Pseudomonas, Pseudovibrio, Vibrio, Thalassiosira, Enhygromyxa and Labrenzia include 217-241. [107][108][109][110][111][112][113][114][115][116] Closer inspection of the spectroscopic and spectrometric data (or lack thereof) presented in several articles reporting new marine bacteria NPs, suggest that the structure assignment of several compounds (202-205, 99 The rst total syntheses of the marine-derived bacteria compounds neomaclafungin A 242, 117 usabamycins A and C, 118 (À)-marinisporolide C, 119 seoaracenes A and B, 120 actinoranone 243, 121 enhygrolide A, 122 discoipyrroles C and D, 123,124 and metagenetriindole A 125 has been achieved.…”

Section: Marine-sourced Bacteriamentioning

confidence: 99%

Marine natural products

et al. 2019

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Section: Marine-sourced Bacteriamentioning

confidence: 99%

Marine natural products

et al. 2019

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“…They are reported to induce pathogen inhibition by antibiosis through the secretion of many secondary metabolites [ 2 ]. Different Bacillus species secrete diverse substances that include surfactins [ 3 ], fengycins [ 4 ], bacisubin [ 5 ] and polyketides [ 6 ]. It has been shown that 3-hydroxypropionaldehyde, produced by Bacillus , efficiently inhibits the growth of Alternaria solani , Botrytis cinerea , Fusarium sambucinum , and Pythium sulcatum [ 7 ].…”

Section: Introductionmentioning

confidence: 99%

Co-culture of Bacillus amyloliquefaciens ACCC11060 and Trichoderma asperellum GDFS1009 enhanced pathogen-inhibition and amino acid yield

Dou

et al. 2018

Microb Cell Fact

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BackgroundBacillus spp. are a genus of biocontrol bacteria widely used for antibiosis, while Trichoderma spp. are biocontrol fungi that are abundantly explored. In this study, a liquid co-cultivation of these two organisms was tried firstly.Results and discussionThrough liquid chromatography-mass spectrometry/mass spectrometry (LC–MS/MS), it was discovered that with an inoculation in the ratio of 1.9:1, the antimicrobial effect of the co-cultured fermentation liquor of Bacillus amyloliquefaciens ACCC11060 and Trichoderma asperellum GDFS1009 was found to be significantly higher than that of pure-cultivation. A raise in the synthesis of antimicrobial substances contributed to this significant increase. Additionally, a co-culture with the inoculation of the two organisms in the ratio of 1:1 was found to enhance the production of specific amino acids. This technique could be further explored for either a large scale production of amino acids or could serve as a theoretical base for the generation of certain rare amino acids.ConclusionsThis work clearly demonstrated that co-cultivation of B. amyloliquefaciens ACCC11060 and T. asperellum GDFS1009 could produce more specific biocontrol substances and amino acids.Electronic supplementary materialThe online version of this article (10.1186/s12934-018-1004-x) contains supplementary material, which is available to authorized users.

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“…Marine Bacillus sp. were accounted for the production of bioactive polyketides, fatty acids, bacteriocins and other unusual compounds of potential clinical significance (Armstrong et al 2001;Thilakan et al 2016;Chakraborty et al 2017a).…”

Section: Introductionmentioning

confidence: 99%

Antibacterial aryl-crowned polyketide from Bacillus subtilis associated with seaweed Anthophycus longifolius

2017

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Aims: Microbiological, biotechnological and chemical characterization of a previously undescribed aryl-crowned polyketide from Bacillus subtilis MTCC 10403 isolated from brown seaweed Anthophycus longifolius with activity against opportunistic Gram-negative food-borne pathogenic bacterial strains. Methods and Results: A culture-dependent method was used to isolate heterotrophic B. subtilis associated with A. longifolius and assessed for its antimicrobial properties. Minimum inhibitory concentration (MIC) of the title compound against the test pathogens was analysed by microtube dilution coupled with the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromidebased colorimetric endpoint detection. Bacillus subtilis MTCC 10403 was found to be antagonistic against Gram-negative food-borne pathogenic Pseudomonas aeruginosa, Escherichia coli, Klebsiella pneumoniae, Salmonella enterica serotype Typhi, Aeromonas hydrophila and Vibrio sp. (diameter of zone of growth inhibition 13-22 mm). Bacillus subtilis was assessed for the presence of secondary metabolite coding polyketide synthase (pks) gene (KC589397, 700-bp gene product) and carboxylate siderophore framework in the aryl-crowned polyketide designated as 7-O-6 0 -(2″-acetylphenyl)-5 0 -hydroxyhexanoate-macrolactin by exhaustive spectroscopic techniques. The MIC assay showed that the reference antibiotics tetracycline and ampicillin were active at 25 lg ml À1 against the test pathogens, whereas the newly isolated polyketide displayed anti-infective properties against E. coli, A. hydrophilla, P. aeruginosa and Vibrio sp. at a lower concentration (MIC <13 lg ml À1 ). The MIC of the aryl macrolactin against K.pneumoniae was comparable with that of the referral antibiotics (~25 lg ml À1 ).The mode of antimicrobial action of acryl-crowned macrolactin was found to be iron chelating similar to siderophores. Putative biosynthetic pathway of the pks gene product further validated its molecular attributions.Conclusions: This study recognized new variant of antimicrobial aryl-crowned polyketide bearing methyl 6 0 -(2″-acetylphenyl)-5 0 -hydroxyhexanoate moiety at the C-7 position of the macrolactin system from A. longifolius-associated bacterium B. subtilis. Significance and Impact of the Study: This study revealed seaweed-associated micro-organisms as promising biological strata to produce new-generation anti-infective agents.

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Antimicrobial polyketide furanoterpenoids from seaweed-associated heterotrophic bacterium Bacillus subtilis MTCC 10403

Cited by 33 publications

References 45 publications

Marine natural products

Marine natural products

Co-culture of Bacillus amyloliquefaciens ACCC11060 and Trichoderma asperellum GDFS1009 enhanced pathogen-inhibition and amino acid yield

Antibacterial aryl-crowned polyketide from Bacillus subtilis associated with seaweed Anthophycus longifolius

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