Antimicrobial Photodynamic Effectiveness of Light Emitting Diode (Led) For Inactivation on Staphylococcus aureus Bacteria and Wound Healing in Infectious Wound Mice

Abstract

This study aims to determine the effectiveness of the antimicrobial photodynamic therapy (aPDT) LEDs of 392 nm and 628 nm red LEDs for photoinactivation on Staphylococcus aureus in vitro and the photodynamic effectiveness of therapy for wound healing in mice in vivo. The sample used was Staphylococcus aureus bacterial isolate which was infected on mice incision wounds. The LED exposure was carried out at the energy density of 19.44 J/cm2, respectively. In vitro test samples were divided into three groups, namely (P0): control group, (P1): LED treatment group 392 nm, (P2): LED treatment group 628 and (P3): LED combination treatment group 392 and 628 nm. In the in vivo test, the mice of infectious wound models were divided into 5 namely (M+): positive control group, (M-): negative control group, (M1): LED treatment group 392 nm, (M2): LED treatment group 628 and (M3): LED combination treatment group 392 and 628 nm. The results of in vitro studies showed an increase in the photoinactivation effect on LED exposure, with the effectiveness of inactivation on the LED 392 nm at 67.10%. The effect of photoinactivation of bacteria on wounds in vivo due to photodynamic therapy in the red LED exposure group was 88%, blue LED exposure group was 94%, exposure combination group of red and blue LEDs was 95%. So, the antimicrobial photodynamic therapy LEDs are effective for bacterial inactivation and accelerate wound healing in mice.

Comparison of the antimicrobial reduction effect of photodynamic inactivation with the addition of chlorophyll and curcumin photosensitizer in Aggregatibacter actinomycetemcomitans and Enterococcus faecalis

“…20,21 The effect of bacterial photoinactivation on wounds in vivo due to photodynamic therapy in the red LED exposure group was 88%, the blue LED exposure group was 94%, and the red and blue LED combination exposure group was 95%. 20 Blue and red lasers combined with ozone treatment effectively accelerated wound healing of incisions infected with MRSA bacteria. 21 Thus, LED antimicrobial photodynamic therapy is effective for bacterial inactivation and accelerates wound healing in mice.…”

“…The photochemical reactions in PDI are generally of the second type. In vivo studies are found in other journals [20,21]. The effect of bacterial photoinactivation on wounds in vivo due to photodynamic therapy in the red LED exposure group was 88%, the blue LED exposure group was 94%, and the red and blue LED combination exposure group was 95% [20].…”

Effectiveness of 650 nm red laser photobiomodulation therapy to accelerate wound healing post tooth extraction

“…Malpositioned teeth can cause trauma to the surrounding soft tissues, and teeth with severe fractures may require extraction. Lastly, extractions may be performed for mouth preparation before prosthodontic treatment [4]. Tooth extraction, or simply extraction, is a procedure that involves separating a tooth from the soft tissue that surrounds it and removing a tooth that cannot be retained within its socket using forceps or an elevator.…”

An in-vivo study of photobiomodulation using 403 nm and 649 nm diode lasers for molar tooth extraction wound healing in wistar rats

“…Wounds can be classified into various degrees, i.e., acute wounds, chronic wounds, and complication wounds, whereas time is an important factor in the wound healing process [4,5]. The process of wound healing in intentional or unintentional tooth extraction differs for each individual.…”

“…This non-thermal therapy uses chromophore as a natural photosensitizing agent in photophysical and photochemical processes at various biological scales [21,22]. Also, it is useful in reducing or relieving pain due to inflammation, regulating immunomodulation, as well as improving wound care and tissue regeneration [5,23].…”