Antimicrobial peptides from different plant sources: Isolation, characterisation, and purification

Tang, Swee-Seong; Prodhan, Zakaria Hossain; Biswas, Sudhangshu Kumar; Le, Cheng Foh; Sekaran, Shamala Devi

doi:10.1016/j.phytochem.2018.07.002

Cited by 105 publications

(65 citation statements)

References 125 publications

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“…Plant‐derived antimicrobials have been researched and consumed frequently over the past decade to improve quality of health and provide ethnopharmacological remedies (Nittayananta, Limsuwan, Srichana, Sae‐Wong, & Amnuaikit, ; Tang, Prodhan, Biswas, Le, & Sekaran, ). Flavonoid, which is the most abundant type of polyphenolic compound found in plant materials, was studied to increase in concentration and antibacterial activity using acid hydrolysis (Min, Kim, Lee, Kim, & Paik, ), fermentation (Saleh et al, ), and silver nanoparticles (Rashid et al, ).…”

Section: Discussionmentioning

confidence: 99%

Inhibitory effects of Inula britannica extract fermented by Lactobacillus plantarum KCCM 11613P on coagulase activity and growth of Staphylococcus aureus including methicillin‐resistant strains

Bae

Kim

et al. 2019

J Food Biochem

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The aim of this study was to evaluate the antimicrobial efficacy of fermented Inula britannica extract (FIBE) against Staphylococcus aureus strains including methicillin‐resistant S. aureus (MRSA). I. britannica extract was fermented by Lactobacillus plantarum KCCM 11613P, and the pathogenicity of S. aureus strains was determined via assessment of coagulase, DNase, and hemolytic activities. Epicatechin concentration increased from 4.38 to 6.05 μg/mg during fermentation (p < 0.01). FIBE treatment inhibited coagulase release from S. aureus to levels below the inhibitory concentration. FIBE promoted the release of intracellular nucleic acids and N‐phenyl‐1‐naphthylamine absorption. In three S. aureus strains, damaged cells exhibited 21.58, 16.79, and 17.65% decreases in membrane potential induced by cell membrane depolarization, respectively (p < 0.05). Upon FIBE treatment in culture, the minimum inhibitory concentration of FIBE exerted a bacteriostatic effect. In conclusion, FIBE possesses antimicrobial properties, including inhibition of virulence factors, damage to cell membranes, and inhibition of bacterial growth. Practical applications Methicillin‐resistant Staphylococcus aureus (MRSA) is a serious concern in hospitals because of its known antibiotic resistance. Vancomycin and tigecycline are used for treating MRSA, but the appearance of vancomycin‐intermediate and multidrug‐resistant strains of these bacteria has created a demand for new antimicrobial agents. This study demonstrates the effective application of Inula britannica and fermentation technology for developing natural antimicrobial agents against methicillin‐resistant Staphylococcus aureus.

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Section: Discussionmentioning

confidence: 99%

Inhibitory effects of Inula britannica extract fermented by Lactobacillus plantarum KCCM 11613P on coagulase activity and growth of Staphylococcus aureus including methicillin‐resistant strains

Bae

Kim

et al. 2019

J Food Biochem

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“…Despite the developments of new antifungal treatments with enhanced potential effects against Aspergillus and Candida species, there are still increasing rates of resistance to the current antifungal treatments resulting in their failure and worsening of clinical outcomes (Wiederhold, ). Therefore, the need for developing new alternative treatments against these fungal infections is highlighted (Tang, Prodhan, Biswas, Le, & Sekaran, ). Among the various types of compounds introduced in this field, antimicrobial peptides are suitable substitutes for traditional antibiotic drugs (Gaiser, Rivas, & López, ).…”

Section: Introductionmentioning

confidence: 99%

“…Therefore, the need for developing new alternative treatments against these fungal infections is highlighted (Tang, Prodhan, Biswas, Le, & Sekaran, 2018). Among the various types of compounds introduced in this field, antimicrobial peptides are suitable substitutes for traditional antibiotic drugs (Gaiser, Rivas, & López, 2011).…”

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confidence: 99%

Isolation, functional characterization, and biological properties of MCh‐AMP1, a novel antifungal peptide from Matricaria chamomilla L.

et al. 2019

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The antimicrobial activities of natural products have attracted much attention due to the increasing incidence of pathogens that have become resistant to drugs. Thus, it has been attempted to promisingly manage infectious diseases via a new group of therapeutic agents called antimicrobial peptides. In this study, a novel antifungal peptide, MCh‐AMP1, was purified by reverse phase HPLC and sequenced by de novo sequencing and Edman degradation. The antifungal activity, safety, thermal, and pH stability of MCh‐AMP1 were determined. This peptide demonstrated an antifungal activity against the tested Candida and Aspergillus species with MIC values in the range of 3.33–6.66 μM and 6.66–13.32 μM, respectively. Further, physicochemical properties and molecular modeling of MCh‐AMP1 were evaluated. MCh‐AMP1 demonstrated 3.65% hemolytic activity at the concentration of 13.32 μM on human red blood cells and 10% toxicity after 48 hr at the same concentration on HEK293 cell lines. The antifungal activity of MCh‐AMP1 against Candida albicans was stable at a temperature range of 30–50°C and at the pH level of 7–11. The present study indicates that MCh‐AMP1 may be considered as a new antifungal agent with therapeutic potential against major human pathogenic fungi.

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“…Plants are constantly exposed to diverse environments harboring various pathogenic microorganisms, such as fungi, bacteria, viruses, protozoa, and nematodes. Therefore, AMPs derived from plants can be used as effective defense molecules against pathogenic microorganisms [22]. The major groups of AMPs found in plants include thionins [22,23], defensins [22,23], and lipid transfer proteins [23,24]; however, reactive oxygen species (ROS)-related proteins were recently included as a new AMP group [6,19].…”

Section: Purification and Identification Of Attrx-h2 -H3 -2n3c Andmentioning

confidence: 99%

Enhanced Antifungal Activity of Engineered Proteins via Swapping between Thioredoxin H2 and H3

et al. 2019

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Thioredoxins (Trxs) are proteins that act as antioxidants by facilitating the reduction of other proteins and are highly conserved in all organisms. Plant H-type Trx isoforms have different structures and perform multiple functions. Previous studies have reported that the low molecular weight AtTrx-H2 acts as a disulfide reductase and the high molecular weight AtTrx-H3 functions as an oxidoreductase and a molecular chaperone. In this study, we compared the antifungal activities of Arabidopsis Trx-H2 and -H3 with engineered proteins 2N3C and 3N2C via domain-swapping between the N- and C-terminal regions of Trx-H2 and -H3. All AtTrx-H variant proteins inhibited cell growth of various pathogenic fungal strains at pH 5.2 and pH 7.2 and showed significant intracellular accumulation in the fungal cells. Interestingly, only two engineered proteins penetrated the fungal cell wall and membrane, indicating their ability to destabilize the fungal cell membrane before internalization into the cytosol. To our knowledge, this is the first study that demonstrates novel functions of plant antioxidants AtTrx-H2 and -H3 as antifungal proteins and shows their enhanced activity using the domain swapping technique.

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Antimicrobial peptides from different plant sources: Isolation, characterisation, and purification

Cited by 105 publications

References 125 publications

Inhibitory effects of Inula britannica extract fermented by Lactobacillus plantarum KCCM 11613P on coagulase activity and growth of Staphylococcus aureus including methicillin‐resistant strains

Inhibitory effects of Inula britannica extract fermented by Lactobacillus plantarum KCCM 11613P on coagulase activity and growth of Staphylococcus aureus including methicillin‐resistant strains

Isolation, functional characterization, and biological properties of MCh‐AMP1, a novel antifungal peptide from Matricaria chamomilla L.

Enhanced Antifungal Activity of Engineered Proteins via Swapping between Thioredoxin H2 and H3

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