Antimicrobial Peptide Functionalized Conductive Nanowire Array Electrode as a Promising Candidate for Bacterial Environment Application

Abstract

Conductive polymer electrodes are widely used for electrical signal detection owing to their unique mechanical, redox, and impedance characteristics. However, the performance of electrodes is compromised due to the interference of adhered bacteria and most of the scientists have not taken the microbial environment into consideration during electrode design. Here, a facile approach to construct antimicrobial peptide (AMP) functionalized polypyrrole nanowire array conductive electrodes (PNW-AMP) is reported. Instead of compromising the electrochemical properties as the other antibacterial agents do, the PNW-AMP electrodes exhibit excellent redox and low interfacial impedance properties. More importantly, the PNW-AMP can eliminate bacterial adhesion and maintain electrochemical stability simultaneously in the microbial microenvironment for a long time. The antibacterial rate of the PNW-AMP electrode reaches 95.8% after exposing the electrode to air for one month, while the charge transfer resistance ( R ct ) value only increases by 9% at a bacterium (Escherichia. Coli ) concentration of 1 × 10 4 colony forming unit (CFU) mL −1 . This research makes it possible to construct highly stable conductive polymer electrodes for bacterial environment electrical signal detection.

Polypyrrole Nanomaterials: Structure, Preparation and Application

“…In recent years, PPy NWs arrays have received attention from researchers and have broad application prospects in the field of supercapacitors and sensors [ 77 , 78 , 79 , 80 , 81 , 82 , 83 , 84 ]. Using oxidative polymerization in air, Kim et al [ 78 ] obtained stretched monomeric menisci by pulling on a micropipette containing a Py solution.…”

Programmable biological state-switching photoelectric nanosheets for the treatment of infected wounds

“…A growing number of studies have demonstrated that certain patterns of electrical stimulation produce cell membrane depolarization and certain prescribed ion movements promote cell proliferation [ [19] , [20] , [21] ]. It also has been found that high intensity electrical stimulation effectively penetrates bacterial walls and destroys bacteria [ 22 , 23 ]. A controlled electrical potential presents the opportunity to integrate antibacterial activity and cell proliferation programmatically via electrical switching.…”

Membrane Stabilization of Poly(ethylene glycol)-b-polypeptide-g-trehalose Assists Cryopreservation of Red Blood Cells

“…As we know, a number of membrane-active peptides or amphipathic polymers with weakly ionizable and hydrophobic groups could interact with the hydrophobic interiors of phospholipid bilayers and subsequently assist membrane permeabilization or fusion, such as antimicrobial peptides and cell-penetrating peptides. − Universally, it was assumed that the balance of hydrophobicity and cationic charge was an essential ingredient for optimization of antimicrobial activity combined with low toxicity to tissue cells . With a similar point of view, the obtained glycopeptides are designed with an expected balance property that glycopeptides interact with the cell membrane to provide membrane stabilization.…”