Antimicrobial metabolites from the aquatic fungus Delitschia corticola

Sun, Rong; Gao, Yanxiu; Shen, Kai-Ze; Xu, Yao-Bo; Wang, Chun-Ren; Liu, Haiyang; Dong, Jinyan

doi:10.1016/j.phytol.2010.12.001

Cited by 20 publications

(18 citation statements)

References 13 publications

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“…Delitschia sp. have been reported from herbivore dung [12,38,39] and submerged wood in freshwater [26,40]. This study provides molecular evidence that Delitschia is not restricted to a coprophilous lifestyle but can also be lignicolous (Fig.…”

Section: Fungal Strain Isolation and Identificationsupporting

confidence: 57%

“…The resultant extract was partitioned using organic solvents and fractionated using flash chromatography, yielding nine fractions. These were purified further using preparative and semipreparative HPLC to yield nine new natural products (1-7, 11, and 15), along with the known compounds 5-(3-S-hydroxybutyl)-4-methoxy-6-methyl-2H-pyran-2-one (8) [22], 5-(3-oxobutyl)-4-methoxy-6-methyl-2H-pyran-2-one (9) [23], pyrenocine I (10) [24], 5-butyl-6-(hydroxymethyl)-4-methoxy-2H-pyran-2-one (12) [23], sporidesmin A (13) [25], 6-ethyl-2,7-dimethoxyjuglone (14) [12,26], artrichitin (16) [27], and lipopeptide 15G256ε (17) [28] (▶ Fig. 2).…”

Section: Resultsmentioning

confidence: 99%

“…sequences from GenBank were aligned using a previously published LSU alignment of members of the Pleosporomycetidae, Dothideomycetes [36]; these included reports of Delitschia sp. from which fungal metabolites have been isolated [12,13,26]. Protocols for DNA extraction, PCR, Sanger sequencing, and phylogenetic analysis have been reviewed [37].…”

Section: Fungal Strain Isolation and Identificationmentioning

confidence: 99%

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Delitpyrones: α-Pyrone Derivatives from a Freshwater Delitschia sp.

et al. 2018

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In research focused on the discovery of new chemical diversity from freshwater fungi, a peak library was built and evaluated against a prostate cancer cell line, E006AA-hT, which was derived from an African American, as this population is disproportionately affected by prostate cancer. The chemical study of the bioactive sample accessioned as G858 ( sp.) led to the isolation of eight new -pyrone derivatives (1: - 7: , and 11: ), as well as the new 3,4-7-ethyl-4,8-dihydroxy-3,6-dimethoxy-3,4-dihydronaphthalen-1()-one (15: ). In addition, the known compounds 5-(3--hydroxybutyl)-4-methoxy-6-methyl-2-pyran-2-one (8: ), 5-(3-oxobutyl)-4-methoxy-6-methyl-2-pyran-2-one (9: ), pyrenocine I (10: ), 5-butyl-6-(hydroxymethyl)-4-methoxy-2-pyran-2-one (12: ), sporidesmin A (13: ), 6-ethyl-2,7-dimethoxyjuglone (14: ), artrichitin (16: ), and lipopeptide 15G256 (17: ) were also obtained. The structures of the new compounds were elucidated using a set of spectroscopic (NMR) and spectrometric (HRMS) methods. The absolute configuration of the most abundant member of each subclass of compounds was assigned through a modified Mosher's ester method. For 15: , the relative configuration was assigned based on analysis of values. Compounds 1, 2, 5: - 14, 16: , and 17: were evaluated against the cancer cell line E006AA-hT under hypoxic conditions, where compound 13: inhibited cell proliferation at a concentration of 2.5 µM.

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Section: Fungal Strain Isolation and Identificationsupporting

confidence: 57%

Section: Resultsmentioning

confidence: 99%

Section: Fungal Strain Isolation and Identificationmentioning

confidence: 99%

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Delitpyrones: α-Pyrone Derivatives from a Freshwater Delitschia sp.

et al. 2018

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“…Several filamentous fungi are cultivated and exploited commercially for the production of enzymes and metabolites. Since the past few years, bioactive compounds have become popular and are the target of numerous surveys (Archer et al, 2008;Sun et al, 2010). In addition, several plants, whose primary mechanism of action may be the inhibition of mycelial growth, are being studied for their fungitoxicity in the control of pathogens (Milanesi et al, 2009).…”

Section: Introductionmentioning

confidence: 99%

Fungistatic activity of essential oil of Lippia sidoides Cham. against Curvularia lunata

Ferreira¹,

MourÃ£o²,

Santos³

et al. 2018

Afr. J. Agric. Res.

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Synthetic pesticides used in agriculture to control pathogens are being widely questioned for their harmful effects on human health and the environment. Therefore, the objective of this study was to evaluate the fungistatic activity of Lippia sidoides Cham. essential oil on alternative control of Curvularia lunata (Wakker), the causal agent of spot in maize plants. The fungitoxic contact effect was successful, resulting in a strong inhibition of C. lunata at the concentration of 50 mg mL −1. Complete adverse effect on the germination of the conidia of C. lunata was obtained at concentrations of 5 and 7.5 mg mL −1. In the preventive assay in maize plants, that is, effect of essential oil on C. lunata, a reduction in the progression of the disease was observed at the oil concentration of 7.5 mg mL −1. Regarding the curative effect in maize plants, no satisfactory result was obtained at concentrations from 0.625 to 7.5 mg mL −1. Cytotoxic test in animal cells was also performed by the viability test using MTT assay, which showed that none of the analyzed concentrations was toxic to the cells. These results demonstrate the potential effect of the essential oil of L. sidoides on the prevention and inhibition of mycelia and conidia germination, controlling the phytopathogenic fungus C. lunata.

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“…procura alternativa de fungos produtores de enzimas, entre os fungos que crescem em condições extremas de temperatura e umidade, é atraente. Pois, estes microrganismos são estimulados a expressar vários metabólitos com a função de promover a adaptação e sobrevivência a estas condições(74). Penicillium tem sido muito utilizado na busca de compostos bioativos.…”

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Purificação de uma beta-galactosidase produzida por aspergillus foetidus através de técnicas cromatográficas

Martarello¹

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Lactose intolerance is characterized by a disorder in absorption of the carbohydrate lactose, which affects thousands of people worldwide and is associated with a deficiency or absence of intestinal β-galactosidase enzyme. In this context, the enzymatic hydrolysis of this disaccharide by β-galactosidase plays an important role in the processing of dairy products with low lactose content for consumption by intolerant individuals. The literature describes that microorganisms such as filamentous fungi can produce enzymes of industrial interest, such as βgalactosidases. Thus the objective of this study was to evaluate the production of extracellular β-galactosidase by filamentous fungi isolated from the soil of the Brazilian savanna in liquid medium using soybean residue as a carbon source. Initially a screening of 25 filamentous fungi strains grown in soybean residue medium was performed assessing β-galactosidase production potential. After selecting Aspergillus foetidus as the best producer, enzyme production was optimized by varying the culture medium followed by an experimental design with response surface analysis with the independent variables pH, temperature and stirring to assess the influence of these physico-chemical parameters in enzyme production. The best β-galactosidase activity (26.4 U/mL) was found in the system containing soybean residues 2% (w/v) at initial pH 7.0 at 28°C with 120 rpm. The induction curve indicated the 7th culture day as the period of highest activity. β-galactosidase was half purified using gel filtration and ion exchange chromatography. The purified enzyme was then characterized aiming its food and pharmaceutical industrial uses with maximal activity at 50°C and pH 3.0. The enzyme hydrolyzed 63% of the lactose present in milk (Molico®), and when tested its stability in gastric simulated conditions it remained active for 2 hours at 37°C. These results demonstrate the half purification of an acidic and stable β-galactosidase from Aspergillus foetidus with potential industrial application from low-cost residue.

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Antimicrobial metabolites from the aquatic fungus Delitschia corticola

Cited by 20 publications

References 13 publications

Delitpyrones: α-Pyrone Derivatives from a Freshwater Delitschia sp.

Delitpyrones: α-Pyrone Derivatives from a Freshwater Delitschia sp.

Fungistatic activity of essential oil of Lippia sidoides Cham. against Curvularia lunata

Purificação de uma beta-galactosidase produzida por aspergillus foetidus através de técnicas cromatográficas

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