Antimicrobial-free graphene nanocoating decreases fungal yeast-to-hyphal switching and maturation of cross-kingdom biofilms containing clinical and antibiotic-resistant bacteria

Agarwalla, Shruti Vidhawan; Ellepola, Kassapa; Sorokin, V. V.; Ihsan, Mario; Silikas, Nick; Neto, AH Castro; Seneviratne, Chaminda Jayampath; Rosa, Vinícius

doi:10.1016/j.bbiosy.2022.100069

Cited by 5 publications

(5 citation statements)

References 50 publications

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“…In the oral environment, more than 500 microbial strains play different roles in the oral biofilm formation and peri-implantitis ( Brunetti et al, 2023 ; Ye et al, 2023 ). Many studies have demonstrated that there is a higher risk of pathogens colonization on the susceptible implant surface during the initial 6 h after surgery ( Jin et al, 2019 ; Agarwalla et al, 2022 ), thus making the antimicrobial effects on the first day vital to clinical success. Hence, two representatives of oral pathogens (Gram-negative Fn and Gram-positive Pg), which are actively implicated in implant-associated infection, were immobilized onto the coatings for 1 d for antibacterial analysis.…”

Section: Discussionmentioning

confidence: 99%

Titanium surfaces with biomimetic topography and copper incorporation to modulate behaviors of stem cells and oral bacteria

Zhou

et al. 2023

Front. Bioeng. Biotechnol.

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Purpose: Insufficient osseointegration and implant-associated infection are major factors in the failure of Ti-based implants, thus spurring scientists to develop multifunctional coatings that are better suited for clinical requirements. Here, a new biomimetic micro/nanoscale topography coating combined with antibacterial copper was simultaneously designed for Ti-based implant surfaces by adopting a hybrid approach combining plasma electrolytic oxidation and hydrothermal treatment.Results: The biological interactions between this biofunctionalized material interface and stem cells promoted cellular adhesion and spreading during initial attachment and supported cellular proliferation for favorable biocompatibility. Bone marrow mesenchymal stem cells (BMMSCs) on the coating displayed enhanced cellular mineral deposition ability, higher alkaline phosphatase activity, and upregulated expression of osteogenic-related markers without the addition of osteoinductive chemical factors, which improved osseointegration. More interestingly, this new coating reduced the viability of oral pathogens (Fusobacterium nucleatum and Porphyromonas gingivalis)—the primary causes of implant-associated infections as indicated by damage of cellular structures and decreased population. This is the first study investigating the antibacterial property of dental implants modified by a hybrid approach against oral pathogens to better mimic the oral environment.Conclusion: These findings suggest that biofunctionalization of the implant coating by surface modification methods and the incorporation of antibacterial copper (Cu) offer superior osteogenesis capability and effective antibacterial activity, respectively. These strategies have great value in orthopedic and dental implant applications.

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Section: Discussionmentioning

confidence: 99%

Titanium surfaces with biomimetic topography and copper incorporation to modulate behaviors of stem cells and oral bacteria

Zhou

et al. 2023

Front. Bioeng. Biotechnol.

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“…This may be explained by the fact that only two out of three possible mechanisms are able to occur, excluding wrapping and trapping [ 51 ]. Graphene nanocoating on titanium surface shows a reduction in adhesion in Candida species, Gram-positive and Gram-negative bacteria and changes in biofilm morphology making it thin and fragmented [ 53 ].…”

Section: Modifications Of Polymer-based Membranesmentioning

confidence: 99%

“…It was obtained by chemical vapor deposition (CVD) where graphene was transferred onto polycarbonate track-etched supports and then etched with oxygen plasma to produce size-selective pores smaller than 1 nm [ 55 ]. What is important for blood-contacting materials, graphene nanocoating shows good hemocompatibility with no hemolytic effect on human erythrocytes after 1h incubation [ 53 ].…”

Section: Modifications Of Polymer-based Membranesmentioning

confidence: 99%

Strategies to Mitigate Biofouling of Nanocomposite Polymer-Based Membranes in Contact with Blood

Wójtowicz,

Stodolak-Zych

2023

Membranes

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An extracorporeal blood purification method called continuous renal replacement therapy uses a porous hollow-fiber polymeric membrane that is exposed to prolonged contact with blood. In that condition, like with any other submerged filtration membrane, the hemofilter loses its properties over time and use resulting in a rapid decline in flux. The most significant reason for this loss is the formation of a biofilm. Protein, blood cells and bacterial cells attach to the membrane surface in complex and fluctuating processes. Anticoagulation allows for longer patency of vascular access and a longer lifespan of the membrane. Other preventive measures include the modification of the membrane itself. In this article, we focused on the role of nanoadditives in the mitigation of biofouling. Nanoparticles such as graphene, carbon nanotubes, and silica effectively change surface properties towards more hydrophilic, affect pore size and distribution, decrease protein adsorption and damage bacteria cells. As a result, membranes modified with nanoparticles show better flow parameters, longer lifespan and increased hemocompatibility.

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“…This may be explained by the fact that only two out of three possible mechanisms are able to occur, excluding wrapping and trapping [51]. Graphene nanocoating on titanium surface shows a reduction in adhesion in Candida species, Grampositive and Gram-negative bacteria and changes in biofilm morphology making it thin and fragmented [53].…”

Section: Graphene Modification Possibilitiesmentioning

confidence: 99%

Strategies to Mitigate Biofouling of Polymer-Based Membranes in Contact with Blood

Wójtowicz,

Stodolak-Zych

2023

Preprint

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In a blood purification technique continuous renal replacement therapy called CRRT , a porous hollow-fiber polymeric membrane is exposed to prolonged contact with blood. In that condition, like with any other submerged filtration membrane, hemofilter loses its properties over time and use which results in a rapid decline in flux. The most significant reason for this loss is the formation of a biofilm. Protein, blood cells and bacterial cells attach to the membrane surface in complex and fluctuating processes. Anticoagulation allows for longer patency of vascular access and a longer lifespan of the membrane. Other preventive measures include modification of the membrane itself. In this article, we focused on the role of nanoadditives in the mitigation of bio-fouling. Nanoparticles such as graphene, carbon nanotubes, silica effectively change surface properties towards more hydrophilic, affect pore size and distribution, decrease protein adsorption and damage bacteria cells. As a result, membranes modified with nanoparticles show better flow parameters, longer lifespan and increased hemocompatibility.

show abstract

Antimicrobial-free graphene nanocoating decreases fungal yeast-to-hyphal switching and maturation of cross-kingdom biofilms containing clinical and antibiotic-resistant bacteria

Cited by 5 publications

References 50 publications

Titanium surfaces with biomimetic topography and copper incorporation to modulate behaviors of stem cells and oral bacteria

Titanium surfaces with biomimetic topography and copper incorporation to modulate behaviors of stem cells and oral bacteria

Strategies to Mitigate Biofouling of Nanocomposite Polymer-Based Membranes in Contact with Blood

Strategies to Mitigate Biofouling of Polymer-Based Membranes in Contact with Blood

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