Antimicrobial films obtained from latex particles functionalized with quaternized block copolymers

Álvarez-Paino, Marta; Juan-Rodríguez, Rafael; Cuervo-Rodríguez, Rocío; Tejero, Rubén; López, Daniel; López-Fabal, Fátima; Gómez-Garcés, José Luís; Muñoz‐Bonilla, Alexandra

doi:10.1016/j.colsurfb.2015.12.031

Cited by 18 publications

(20 citation statements)

References 31 publications

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“…Scheme 1 illustrates the synthetic approach used for the synthesis of the cationic copolymers. The preparation of the antimicrobial copolymers was carried out by combination of ATRP and copper‐catalyzed azide‐alkyne cycloaddition (CuAAC) click reaction to obtain well‐defined copolymers, followed by a quaternization step . TTBM monomer is synthesized by click chemistry between HMA and 2‐(4‐methylthiazol‐5‐yl)ethanol azide using CuCl/PMDETA as catalyst system.…”

Section: Resultsmentioning

confidence: 99%

“…The reactions were monitored by 1 H‐NMR, taking samples of the reaction mixture at set time intervals. It was appreciated the signals corresponding to the protons of thiazole rings at the beginning of the reaction, which indicates that ‘click’ reaction is almost instantaneously completed, whereas the vinyl signals at ≈6.0 and 5.5 ppm decrease continuously during the course of the polymerization . After 6.5 h the reaction was stopped and the TTBM monomer conversion was determined before purification, using the relative intensity of the signals from the vinyl protons (6.0 and 5.5 ppm) and α‐methyl hydrogens of the polymer (≈1.1–0.5 ppm), resulting ≈30% in both cases.…”

Section: Resultsmentioning

confidence: 99%

“…The block copolymers were synthesized following a one‐step process, simultaneous “click chemistry”/ATRP . Initially, a ratio [monomer]:[macroinitiator]:[azide]:[CuCl]:[PMDETA] = 75:1:150:1:4 was used.…”

Section: Methodsmentioning

confidence: 99%

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Contact Active Antimicrobial Coatings Prepared by Polymer Blending

Cuervo-Rodríguez

López-Fabal

Gómez-Garcés

et al. 2017

Macromolecular Bioscience

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Herein, contact active antimicrobial films are prepared by simply blending cationic amphiphilic block copolymers with commercial polystyrene (PS). The copolymers are prepared by combining atom transfer radical polymerization and "click chemistry." A variety of copolymers are synthesized, and composed of a PS segment and an antimicrobial block bearing flexible side chain with thiazole and triazole groups, 4-(1-(2-(4-methylthiazol-5-yl)ethyl)-1H-1,2,3-triazol-4-yl) butyl methacrylate (TTBM). The length of the TTBM block is varied as well as the alkylating agent. Different films are prepared from N,N-dimethylformamide solution, containing variable PS-b-PTTBM/PS ratio: from 0 to 100 wt%. Remarkably, the blend films, especially those with 30 and 50 wt% of copolymers, exhibit excellent antimicrobial activities against Gram-positive, Gram-negative bacteria and fungi, even higher than films prepared exclusively from the cationic copolymers. Blends composed of 50 wt% of the copolymers present a more than 99.999% killing efficiency against the studied microorganisms. The better activity found in blends can be due to the higher roughness, which increases the surface area and consequently the contact with the microorganisms. These results demonstrate that the use of blends implies a reduction of the content of antimicrobial agent and also enhances the antimicrobial activity, providing new insights for the better designing of antimicrobial coatings.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

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Contact Active Antimicrobial Coatings Prepared by Polymer Blending

Cuervo-Rodríguez

López-Fabal

Gómez-Garcés

et al. 2017

Macromolecular Bioscience

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“…Inhibition of bacterial growth on the surfaces of medical equipment and devices is necessary to prevent the transmission of diseases by contact, and one promising approach is the development of antimicrobial coatings. Many of these self-disinfecting coatings are based on impregnating the surfaces with antimicrobial agents including antibiotics, silver compounds, light active species, and antimicrobial polymers such as polycations [3,4,5,6,7]. Other strategies limit the bacterial colonization of surfaces by inducing micro and nano-roughness, which modifies the surface area of contact with the microorganisms [8,9,10].…”

Section: Introductionmentioning

confidence: 99%

Antimicrobial Porous Surfaces Prepared by Breath Figures Approach

et al. 2018

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Herein, efficient antimicrobial porous surfaces were prepared by breath figures approach from polymer solutions containing low content of block copolymers with high positive charge density. In brief, those block copolymers, which were used as additives, are composed of a polystyrene segment and a large antimicrobial block bearing flexible side chain with 1,3-thiazolium and 1,2,3-triazolium groups, PS54-b-PTTBM-M44, PS54-b-PTTBM-B44, having different alkyl groups, methyl or butyl, respectively. The antimicrobial block copolymers were blended with commercial polystyrene in very low proportions, from 3 to 9 wt %, and solubilized in THF. From these solutions, ordered porous films functionalized with antimicrobial cationic copolymers were fabricated, and the influence of alkylating agent and the amount of copolymer in the blend was investigated. Narrow pore size distribution was obtained for all the samples with pore diameters between 5 and 11 µm. The size of the pore decreased as the hydrophilicity of the system increased; thus, either as the content of copolymer was augmented in the blend or as the copolymers were quaternized with methyl iodide. The resulting porous polystyrene surfaces functionalized with low content of antimicrobial copolymers exhibited remarkable antibacterial efficiencies against Gram positive bacteria Staphylococcus aureus, and Candida parapsilosis fungi as microbial models.

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“…The Therefore, these results demonstrated that the mechanism of action of these antimicrobial surfaces is by cell-membrane disrupting due to hydrophobic interactions and electrostatic interactions between the negatively charged cell walls and the positively charged copolymers [24][25][26][27] .…”

Section: Evaluation Of the Morphological Changes Of Microbesmentioning

confidence: 99%

Tailoring Macromolecular Structure of Cationic Polymers towards Efficient Contact Active Antimicrobial Surfaces

Tejero¹,

Gutiérrez²,

López³

et al. 2018

Preprint

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The aim of this work is the preparation of contact active antimicrobial films by blending copolymers with quaternary ammonium salts and polyacrylonitrile as matrix material. A series of copolymers based on acrylonitrile and methacrylic monomers with quaternizable groups were designed with the purpose of investigating the influence of their chemical and structural characteristics on the antimicrobial activity of these surfaces. The biocide activity of these systems was studied against different microorganisms, such as the Gram-positive bacteria Staphylococcus aureus and the Gram-negative bacteria Pseudomona aeruginosa and the yeast Candida parapsilosis. The results confirmed that parameters such as flexibility and polarity of the antimicrobial polymers immobilized on the surfaces strongly affect the efficiency against microorganisms. In contrast to the behavior of copolymers in water solutions, when they are tethered to the surface, the active cationic groups are less accessible and then the mobility of the side chain is critical for a good contact with the microorganism. Blend films composed of copolymers with high positive charge density and chain mobility present up to a more than 99.999% killing efficiency against the studied microorganisms.Preprints (www.preprints.org) | NOT PEER-REVIEWED | Posted:

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Antimicrobial films obtained from latex particles functionalized with quaternized block copolymers

Cited by 18 publications

References 31 publications

Contact Active Antimicrobial Coatings Prepared by Polymer Blending

Contact Active Antimicrobial Coatings Prepared by Polymer Blending

Antimicrobial Porous Surfaces Prepared by Breath Figures Approach

Tailoring Macromolecular Structure of Cationic Polymers towards Efficient Contact Active Antimicrobial Surfaces

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