“…Silver nanoparticles have been extensively studied with regard to their unique physical and chemical properties, which are greatly affected by shape, size, and dispersion stability of particles [31,32]. Nanoparticles could be more efficiently formed to deliver themselves to target organisms [33]. Studiess on the electrospun Ag nanoparticle-embedded polymer nanofibers using the mixture of AgNO 3 precursor and polymer solutions, i.e., PVA [34], PVP [3,33], poly (lactide- co -glycolide) (PLGA) [35], PEO [36,37], and PAN [38,39] exhibited the improved antimicrobial properties in composites.…”
Poly (vinyl pyrrolidone) (PVP)/cellulose nanocrystal (CNC)/silver nanoparticle composite fibers were prepared via electrospinning using N,N′-dimethylformamide (DMF) as a solvent. Rheology, morphology, thermal properties, mechanical properties, and antimicrobial activity of nanocomposites were characterized as a function of material composition. The PVP/CNC/Ag electrospun suspensions exhibited higher conductivity and better rheological properties compared with those of the pure PVP solution. The average diameter of the PVP electrospun fibers decreased with the increase in the amount of CNCs and Ag nanoparticles. Thermal stability of electrospun composite fibers was decreased with the addition of CNCs. The CNCs help increase the composite tensile strength, while the elongation at break decreased. The composite fibers included Ag nanoparticles showed improved antimicrobial activity against both the Gram-negative bacterium Escherichia coli (E. coli) and the Gram-positive bacterium Staphylococcus aureus (S. aureus). The enhanced strength and antimicrobial performances of PVP/CNC/Ag electrospun composite fibers make the mat material an attractive candidate for application in the biomedical field.
“…Silver nanoparticles have been extensively studied with regard to their unique physical and chemical properties, which are greatly affected by shape, size, and dispersion stability of particles [31,32]. Nanoparticles could be more efficiently formed to deliver themselves to target organisms [33]. Studiess on the electrospun Ag nanoparticle-embedded polymer nanofibers using the mixture of AgNO 3 precursor and polymer solutions, i.e., PVA [34], PVP [3,33], poly (lactide- co -glycolide) (PLGA) [35], PEO [36,37], and PAN [38,39] exhibited the improved antimicrobial properties in composites.…”
Poly (vinyl pyrrolidone) (PVP)/cellulose nanocrystal (CNC)/silver nanoparticle composite fibers were prepared via electrospinning using N,N′-dimethylformamide (DMF) as a solvent. Rheology, morphology, thermal properties, mechanical properties, and antimicrobial activity of nanocomposites were characterized as a function of material composition. The PVP/CNC/Ag electrospun suspensions exhibited higher conductivity and better rheological properties compared with those of the pure PVP solution. The average diameter of the PVP electrospun fibers decreased with the increase in the amount of CNCs and Ag nanoparticles. Thermal stability of electrospun composite fibers was decreased with the addition of CNCs. The CNCs help increase the composite tensile strength, while the elongation at break decreased. The composite fibers included Ag nanoparticles showed improved antimicrobial activity against both the Gram-negative bacterium Escherichia coli (E. coli) and the Gram-positive bacterium Staphylococcus aureus (S. aureus). The enhanced strength and antimicrobial performances of PVP/CNC/Ag electrospun composite fibers make the mat material an attractive candidate for application in the biomedical field.
“…The live/dead staining technique contains two nucleic dyes, SYTO9 and propidium iodide (PI), to quantify live and dead microbes from the fluorescence microscopy images. 11 The SYTO9 can enter bacterial cell membrane and label DNA to produce green color for living bacteria, while PI only binds to the nucleic acid of the dead cells with destroyed membrane and shows red fluorescence. As shown in Figure 5A, the number of live bacteria decreased with prolonged sterilization.…”
Section: Antibacterial Properties Of Vancomycinmodified Fe 3 O 4 @Siomentioning
confidence: 99%
“…5 Many nanomaterials, including gold, 6 silver, 7 copper, 8 zinc oxide, 9 and graphene oxide, 10 exhibit excellent bactericidal activity; of these, Ag nanomaterials possess the optimal antibacterial activity because they kill most pathogenic microbes, including bacteria, fungi, and viruses. 11,12 Thus far, no organism has readily developed resistance to Ag nanomaterials. 13 Moreover, Thus, various Ag-based nanoparticles have been extensively studied as potent and broad-spectrum antimicrobial agents.…”
Nanomaterials combined with antibiotics exhibit synergistic effects and have gained increasing interest as promising antimicrobial agents. In this study, vancomycin-modified magnetic-based silver microflowers (Van/Fe
3
O
4
@SiO
2
@Ag microflowers) were rationally designed and prepared to achieve strong bactericidal ability, a wide antimicrobial spectrum, and good recyclability. High-performance Fe
3
O
4
@SiO
2
@Ag microflowers served as a multifunction-supporting matrix and exhibited sufficient magnetic response property due to their 200 nm Fe
3
O
4
core. The microflowers also possessed a highly branched flower-like Ag shell that provided a large surface area for effective Ag ion release and bacterial contact. The modified-vancomycin layer was effectively bound to the cell wall of bacteria to increase the permeability of the cell membrane and facilitate the entry of the Ag ions into the bacterium, resulting in cell death. As such, the fabricated Van/Fe
3
O
4
@SiO
2
@Ag microflowers were predicted to be an effective and environment-friendly antibacterial agent. This hypothesis was verified through sterilization of Gram-negative
Escherichia coli
and Gram-positive methicillin-resistant
Staphylococcus aureus
, with minimum inhibitory concentrations of 10 and 20 μg mL
−1
, respectively. The microflowers also showed enhanced effect compared with bare Fe
3
O
4
@SiO
2
@Ag microflowers and free-form vancomycin, confirming the synergistic effects of the combination of the two components. Moreover, the antimicrobial effect was maintained at more than 90% after five cycling assays, indicating the high stability of the product. These findings reveal that Van/Fe
3
O
4
@SiO
2
@Ag microflowers exhibit promising applications in the antibacterial fields.
“…25 The insitu reduction of silver with UV irradiation was proposed in PVA or polyvinylpyrrolidone (PVP) prepared by electrospinning. 26,27 Ascorbyl palmitate, a derivative of vitamin C, was employed to reduce silver ions into silver nanoparticles in nanofibrous mats made of electrospun poly(e-caprolactone) (PCL). 28 The deposits of elemental silver were clearly observed on the surface of the fibers as aggregates of nanoparticles of an average size of »30 nm (Fig.…”
Electrospinning is a versatile technique providing highly tunable nanofibrous nonwovens. Many biomedical applications have been developed for nanofibers, among which the production of antimicrobial mats stands out. The production of scaffolds for tissue engineering, fibers for controlled drug release, or active wound dressings are active fields of research exploiting the possibilities offered by electrospun materials. The fabrication of materials for active food packaging or membranes for environmental applications is also reviewed. We attempted to give an overview of the most recent literature related with applications in which nanofibers get in contact with living cells and develop a nano-bio interface.
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