Antimicrobial electrospun poly(ε-caprolactone) scaffolds for gingival fibroblast growth

Baranowska‐Korczyc, Anna; Warowicka, Alicja; Jasiurkowska-Delaporte, Małgorzata; Grześkowiak, Bartosz F.; Jarek, Marcin; Maciejewska, Barbara M.; Jurga-Stopa, Justyna; Jurga, Stefan

doi:10.1039/c6ra02486f

Cited by 63 publications

(36 citation statements)

References 46 publications

(47 reference statements)

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“…To demonstrate how the analysis is performed we focus on the carbonyl stretch region of the spectrum, which has been used as a crystallinity indicator in previous studies with a single component in the melt state. [11–13]…”

Section: Resultsmentioning

confidence: 99%

“…Potential crystallinity indicators have been identified for vibrational modes corresponding to C-COO stretch, C-C stretch, CH 2 twist, CH 2 bend, and carbonyl (C=O) stretch modes. [9] Peak height ratios in the CH 2 twist region[10] and peak deconvolution of the carbonyl stretch region[11–13] have been used to qualitatively assess crystallinity without quantitative verification.…”

Section: Introductionmentioning

confidence: 99%

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Determining conformational order and crystallinity in polycaprolactone via Raman spectroscopy

Kotula

Snyder

Migler

2017

Polymer

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Raman spectroscopy is a popular method for non-invasive analysis of biomaterials containing polycaprolactone in applications such as tissue engineering and drug delivery. However there remain fundamental challenges in interpretation of such spectra in the context of existing dielectric spectroscopy and differential scanning calorimetry results in both the melt and semi-crystalline states. In this work, we develop a thermodynamically informed analysis method which utilizes basis spectra – ideal spectra of the polymer chain conformers comprising the measured Raman spectrum. In polycaprolactone we identify three basis spectra in the carbonyl region; measurement of their temperature dependence shows that one is linearly proportional to crystallinity, a second correlates with dipole-dipole interactions that are observed in dielectric spectroscopy and a third which correlates with amorphous chain behavior. For other spectral regions, e.g. C-COO stretch, a comparison of the basis spectra to those from density functional theory calculations in the all-trans configuration allows us to indicate whether sharp spectral peaks can be attributed to single chain modes in the all-trans state or to crystalline order. Our analysis method is general and should provide important insights to other polymeric materials.

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Determining conformational order and crystallinity in polycaprolactone via Raman spectroscopy

Kotula

Snyder

Migler

2017

Polymer

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“…Novel fibrous scaffolds of polymers such as PCL and PLA fabricated via electrospinning have been reported for tissue engineering constructs (Baranowska‐Korczyc et al, ; Zhao et al, ). Most native ECMs found in tissues or organs have a regular or defined orientation architecture, which is significant for tissue function.…”

Section: Discussionmentioning

confidence: 99%

“…However, the synergistic effect of chemistry and topography on cell behavior has not yet been wellexplored. Herein, selected fundamental issues that should be consid- Novel fibrous scaffolds of polymers such as PCL and PLA fabricated via electrospinning have been reported for tissue engineering constructs (Baranowska-Korczyc et al, 2016;Zhao et al, 2014). Most native ECMs found in tissues or organs have a regular or defined orientation architecture, which is significant for tissue function.…”

Section: Discussionmentioning

confidence: 99%

Synergetic effect of chemical and topological signals of gingival regeneration scaffold on the behavior of human gingival fibroblasts

Yang

Guo

et al. 2019

J Biomedical Materials Res

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Achievement of gingiva regeneration poses a substantial challenge for dental aesthetics and periodontal disease repair, but reports of a bioactive and easily available gingival regeneration scaffold are rare. Cell behaviors can be affected by multiple kinds of bioactive signals; thus, it is important to explore the effects of the chemical and topological signals of the scaffold on the behavior of human gingival fibroblasts (HGFs). We hypothesized that the synergetic effect of the chemical and topological scaffold signals were beneficial to gingival regeneration. In this study, HGF behavior on random/aligned poly (e‐caprolactone) (PCL) and polydopamine (PDA)‐coated PCL electrospun scaffolds was investigated in a common medium and in basic fibroblast growth factor (bFGF) medium. The results showed that the synergistic effect of three signals was better than that of one or two signals. The cell proliferation of the aligned scaffold group was higher than that of the random scaffold, and the PCL/PDA‐Aligned+bFGF group showed the highest cell proliferation. Even if two chemical signals were present, the HGFs still maintained an ordered distribution on the aligned scaffold. Cell differentiation and protein secretion analysis indicated that gene and protein expression of focal adhesion kinase and fibronectin were the highest in the PCL/PDA‐Aligned+bFGF group. Taken together, the chemical and topographic signals within the electrospun scaffold were considered to display a synergistic effect on HGF behaviors, suggesting the potential usefulness of the PCL/PDA‐Aligned+bFGF scaffold for gingiva tissue engineering.

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“…Przykładowo, Baranowska-Korczyc i współpr. wytworzyli nanowłókna z PCL, które zastosowano jako podstawy wzrostu fibro blastów dziąsłowych [51]. Do nanowłókien wprowadzo no substancję aktywną -ampicylinę.…”

Section: Systemy Uwalniania Lekówunclassified

Electrospun polymer nanofibers for medical applications

Winkler¹,

Maliszewska²,

Czapka³

2020

Polimery

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Streszczenie: Nanowłókna polimerowe wzbudzają obecnie ogromne zainteresowanie ze względu na ich potencjalne wykorzystanie w różnych procesach technologicznych, np. w produkcji tkanin lub wy twarzaniu membran. Włókna te wykazują wyjątkowe właściwości, takie jak: duży stosunek powierzch ni do objętości oraz duża porowatość. Znanych jest kilka metod wytwarzania nanowłókien, jednak ze względu na prostotę, powtarzalność i niewielkie koszty, najpowszechniej stosowane jest przędzenie elektrostatyczne. Przedstawiono przegląd najnowszych osiągnięć w zakresie zastosowań nanowłókien polimerowych w medycynie, obejmujący zagadnienia materiałów opatrunkowych, uwalniania sub stancji aktywnych oraz inżynierii tkankowej.Słowa kluczowe: elektroprzędzenie, nanowłókna, zastosowania medyczne.Abstract: Polymer nanofibers are currently of great interest in terms of their potential use in various technological processes, e.g. in the manufacture of textiles or membranes. These fibers are characterized by extraordinary properties such as high surface to volume ratio and high porosity. There are several methods of manufacturing nanofibers, but for reasons of simplicity, repeatability and low cost, elec trostatic spinning is the most common. The article presents a review of the latest developments in the application of polymer nanofibers in medicine, including such issues as bandage materials, release of active substances and tissue engineering.

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Antimicrobial electrospun poly(ε-caprolactone) scaffolds for gingival fibroblast growth

Abstract: This study discusses the value of polymer electrospun materials in three-dimensional (3D) scaffolds and antibacterial wound dressings for potential dental applications.

Cited by 63 publications

References 46 publications

Determining conformational order and crystallinity in polycaprolactone via Raman spectroscopy

Determining conformational order and crystallinity in polycaprolactone via Raman spectroscopy

Synergetic effect of chemical and topological signals of gingival regeneration scaffold on the behavior of human gingival fibroblasts

Electrospun polymer nanofibers for medical applications

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