Antimicrobial efficacy of photodynamic therapy, Nd:YAG laser and QMiX solution against Enterococcus faecalis biofilm

Jurič, Ivona Bago; Plečko, Vanda; Anić, Ivica; Pleško, Sanja; Jakovljević, Suzana; Rocca, Jean‐Paul; Medioni, Etienne

doi:10.1016/j.pdpdt.2015.07.176

Cited by 20 publications

(10 citation statements)

References 29 publications

(44 reference statements)

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“…The results of studies by da Forta (31), Silva (32), Marinic (33) and Juric (34) in relation to the effect of photodynamic therapy compared to the positive control group on E. faecalis are consistent with the results of the present study; all the studies above have used microbial culture and colony count technique.…”

Section: Discussionsupporting

confidence: 90%

Evaluation of Antibacterial Efficacy of Photodynamic Therapy vs. 2.5% NaOCl against E. faecalis-infected Root Canals Using Real-time PCR Technique

Janani¹,

Jafari²,

Samiei³

et al. 2017

J Clin Exp Dent

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BackgroundBacteria like E. faecalis can produce intra- and extra-radicular biofilms. Theoretically, the adjustable penetration ability of lasers enables better access to root canal system. Therefore the aim of the present study was to compare the ability of photoactivated laser and 2.5% NaOCl irrigation solution to eliminate E. faecalis from the root canals by real-time PCR technique.Material and MethodsSixty extracted human upper central incisors were selected and sterilized in an autoclave. The root canals were infected with E. faecalis (PTCC 1237, Persian Type Culture Collection, Iran) and then incubated for 24 hours. The samples were randomly divided into 3 groups. No intervention was made in the control group (group 1). In group 2, laser therapy was performed with a power of 100 mW by diode laser for 120 seconds. In group 3, the canals were irrigated with 5 mL of 2.5% NaOCl; then all the samples were sonicated in 15 mL of normal saline in test tubes in order to isolate the bacteria. DNA extraction was performed followed by real-time PCR technique for all the samples.ResultsInhibition of bacterial growth in all the experimental samples was significantly more than that in the control group. There was a significant difference between photodynamic therapy and 2.5% NaOCl. The effect of NaOCl in all the samples was better than photodynamic therapy. The results of the mean CT (cyclic threshold) were 40, 30.2 and 15.35 for 2.5% NaOCl, photodynamic therapy and control group, respectively.ConclusionsBased on the results of this experimental study, 2.5% NaOCl eliminated E. faecalis from infected root canals more effectively compared to photodynamic therapy. Key words:Photoactivated laser, Enterococcus faecalis, antibacterial agents, sodium hypochlorite.

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Section: Discussionsupporting

confidence: 90%

Evaluation of Antibacterial Efficacy of Photodynamic Therapy vs. 2.5% NaOCl against E. faecalis-infected Root Canals Using Real-time PCR Technique

Janani¹,

Jafari²,

Samiei³

et al. 2017

J Clin Exp Dent

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“…Ultrasonic activation causes an acoustic flow in the irrigation solution due to rapid circular movement 14 . Although the effectiveness of LAI and PUI for irrigation activation in eliminating bacteria is well known, the effect on the bond strength of post to root dentin after use is not known 27 . The findings of this study showed that the combination of NaOCl/EDTA activation with Nd: YAG laser and PUI had no significant effect on bonding strength when compared with the NaOCl/EDTA combination alone.…”

Section: Dıscussıonmentioning

confidence: 99%

Effect of ultrasonic and Nd: Yag laser activation on irrigants on the push-out bond strength of fiber post to the root canal

Küçükekenci

2019

J. Appl. Oral Sci.

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Objective: This in vitro study aimed to compare the efficacy of irrigants using various irrigation activation methods to the push-out bond strengths of fiber post to root canal luted with self-adhesive resin cement (SARC). Methodology: Forty-eight decoronated human canines were used. The specimens were divided into four groups corresponding with the post-space irrigation process and were treated as follows: distilled water (DW) (Control) group received 15 mL of DW; sodium hypochlorite (NaOCl)+ethylenediaminetetraacetic acid (EDTA) group was treated with 5 mL of 5.25% NaOCl, 5 mL of 17% EDTA, and 5 mL of DW; passive ultrasonic irrigation (PUI) group was treated with 5 mL of 5.25% NaOCl, 5 mL of 17% EDTA, and 5 mL of DW, and each irrigant was agitated with an ultrasonic file; and laser activated irrigation (LAI) group was treated with 5 mL of 5.25% NaOCl, 5 mL of 17% EDTA, and 5 mL of DW, and each irrigant was irradiated with Nd: YAG laser. Fiber posts were luted with SARC, and a push-out test was performed. Data was analyzed using one-way analysis of variance and Tukey HSD test. Results: The bond strength values for the groups obtained were as follows: Control (10.04 MPa), NaOCl+EDTA (11.07 MPa), PUI (11.85 MPa), and LAI (11.63 MPa). No statistically significant differences were found among all experimental groups (p>0.05). The coronal (12.66 MPa) and middle (11.63 MPa) root regions indicated a significantly higher bond strength compared with the apical (9.16 MPa) region (p<0.05). Conclusions: Irrigant activation methods did not increase the bond strength of fiber post to canal.

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“…Bakterilerden Propionibacterium acnes (22) ; Aggregatibacter actinomycetemcomitans, Porphyromonas gingivalis, Treponema denticola ve Tannerella forsythia'nın (9) yanı sıra bir fungus olan Paracoccidioides spp. (4) ve S.aureus (40) ile Enterococcus faecalis'in (2) oluşturduğu biyofilmleri inaktive eden boya türevli FS ajanlar ile ilgili çalışmalar bulunmaktadır. Ayrıca yoğun bakım ünitelerindeki hastalarda nozokomiyal pnömoni riskini azaltmak ve önlemek için oral kolonizasyonuna karşı yeni önleme stratejileri arasında FDAT gösterilmektedir (23) .…”

Section: Fotodinamik Antimikrobiyal Tedavinin Kullanım Alanlarıunclassified

“…ile çoklu ilaç direnci göste-ren Acinetobacter spp. ve Pseudomonas aeruginosa suşlarının neden olduğu enfeksiyonların tedavisi zor olduğundan; bu bakteriler yeni antimikrobiyal ajanların en önemli hedefleri arasında yer almaktadır (2,40,42) . Fotodinamik antimikrobiyal tedavi (FDAT) son yıllarda bakteriler, virüsler ve mantarlar tarafından meydana gelen enfeksiyonların tedavisinde uygulanan noninvazif bir yaklaşım olup, antibiyotiklerin kullanımına alternatif olabilecek yeni yaklaşımlar sunmaktadır.…”

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Photoantimicrobials and Photodynamic Antimicrobial Therapy in Search of Antibiotics

Börekçi¹,

Eliuz²

2017

Ankem Derg

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Antimicrobial efficacy of photodynamic therapy, Nd:YAG laser and QMiX solution against Enterococcus faecalis biofilm

Cited by 20 publications

References 29 publications

Evaluation of Antibacterial Efficacy of Photodynamic Therapy vs. 2.5% NaOCl against E. faecalis-infected Root Canals Using Real-time PCR Technique

Evaluation of Antibacterial Efficacy of Photodynamic Therapy vs. 2.5% NaOCl against E. faecalis-infected Root Canals Using Real-time PCR Technique

Effect of ultrasonic and Nd: Yag laser activation on irrigants on the push-out bond strength of fiber post to the root canal

Photoantimicrobials and Photodynamic Antimicrobial Therapy in Search of Antibiotics

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