Antimicrobial efficacy of Novel Ethanolic Extract of Morinda Citrifolia Against Enterococcus Feacalis by Agar Well Diffusion Method and Minimal Inhibitory Concentration- An Invitro Study.

Abstract

Introduction: the long term success of root canal treatment is ultimately related to the effective debridement and disinfection of the root canal system. Hence, the irrigants play an important role in achieving the good penetrability and bactericidal activity. The present study was mainly aimed at evaluating the invitro antimicrobial efficacy of Novel Ethanolic Extract of Morinda Citrifolia by agar well diffusion and minimal inhibitory concentration. Materials and Methods:the antimicrobial efficacy of the Novel Ethanolic Extract of Morinda Citrifolia was tested using agar well diffusion and minimal inhibitory concentration was assessed. The zone of inhibitions were determined at 10 mg/ml concentration of Ethanolic Extract of Morinda Citrifolia on agar well plate and Minimal Inhibitory Concentration (MIC) against tested microorganism. Results: results obtained in the present study by both qualitative and quantitative experiments revieled that the tested Ethanolic Extract of Morinda Citrifolia possesses potential antibacterial activity against Enterococcus Feacalis when compared with standard antibiotic tetracycline. the highest zone of inhibition of 15mm was showed at 1000micrograms by agar well diffusion assay. The optimal antimicrobial activity was seen at 250micrograms for Morinda Citrifolia against Enterococcus Feacalis.Conclusion: novel Ethanolic extract of Morinda Citrifolia has shown an optimal antimicrobial activity against E.Feaclais. But still, future studies are still needed.

Comparative evaluation of inter appointment pain and analgesic intake with calcium hydroxide and triple antibiotic paste as intracanal medicaments in patients with apical periodontitis a randomized controlled single-blinded clinical trial

“…Usage of antibiotics is only advised in cases which are reluctant to the normal mode of the therapy performed. Literature also supports the use of a wide range of natural plant extracts to be effective and exhibiting the antimicrobial properties [26,27]. So, to address the current threat on the development of antibiotic resistance, other adjunctive therapies should be concentrated more rather relying on the conventional mode of antibiotic administration.…”

Antibacterial prophylaxis and molecular docking studies of ketone and ester compounds isolated from Cyperus rotundus L. against Aeromonas veronii

“…The agar well diffusion technique was used to conduct the antibacterial test against A. veronii under the National Committee for Clinical Laboratory Standards (NCCLS, 1993;Gummuluri et al, 2019). The CrMF6 fraction and the control were both dissolved in 5% dimethyl sulfoxide (DMSO).…”

Antibacterial Efficacy of Conventional Versus Herbal Products on Streptococcus mutans in Adult Population- a Systematic Review & Meta-analysis

“…Literature favors a wide range of natural plant extracts exhibiting antimicrobial properties and therapeutic benefits [11,12]. Considering this fact, many clinicians have shifted their interest produtos naturais exerceram efeito antibacteriano homogêneo contra o Streptococcus mutans quando comparados com a contraparte.…”

“…towards exploring the natural plant extracts [11]. Especially in dentistry, interest towards the usage of herbal products has increased.…”