Antimicrobial Efficacy of Metal-Barbiturate Conjugates Against Pathogenic Strains of Escherichia coli and Staphylococcus aureus

Qureshi, Ashfaq Mahmood; Qadir, Mehmood; Idrees, Muhammad; Mumtaz, Siara; Najam‐ul‐Haq, Muhammad; Ismail, Muhammad Owais; Athar, Makshoof; Khushal, Rashid; Riaz, Sara; Bokhari, Habib

doi:10.2174/157018011797655232

Cited by 7 publications

(1 citation statement)

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“…Using varying percentages of DMSO as the test compound and a blank control with only broth as the reference solution, IC 50 curves at 34, 37 and 40 °C were constructed ( Figure 2 ). It was found that the IC 50 of DMSO on S. aureus is 6.00 ± 0.07% v / v , which is in accordance with a previous study of the effect of DMSO on S. aureus viability [ 25 ]. Based on these findings, it was decided to use a DMSO concentration of 5% v/v in the remaining experiments as a compromise between S. aureus inhibition and the ability to sufficiently dissolve the crude extracts.…”

Section: Resultssupporting

confidence: 90%

Coupling Microplate-Based Antibacterial Assay with Liquid Chromatography for High-Resolution Growth Inhibition Profiling of Crude Extracts: Validation and Proof-of-Concept Study with Staphylococcus aureus

Ardalani

Anam

Olsen³

et al. 2021

Molecules

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With the identification of novel antibiotics from nature being pivotal in the fight against human pathogenic bacteria, there is an urgent need for effective methodologies for expedited screening of crude extracts. Here we report the development and validation of a simple and dye-free antimicrobial assay in 96-well microplate format, for both determination of IC50 values and high-resolution inhibition profiling to allow pin-pointing of bioactive constituents directly from crude extracts. While commonly used antimicrobial assays visualize cell viability using dyes, the developed and validated assay conveniently uses OD600 measurements directly on the fermentation broth. The assay was validated with an investigation of the inhibitory activity of DMSO against Staphylococcus aureus, temperature robustness, interference by coloured crude extracts as well as inter-day reproducibility. The potential for high-resolution S. aureus growth inhibition profiling was evaluated on a crude extract of an inactive Alternaria sp., spiked with ciprofloxacin.

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Section: Resultssupporting

confidence: 90%