Antimicrobial efficacy and biocompatibility study of copper nanoparticle adsorbed mullite aggregates

Bagchi, Biswajoy; Dey, Sumit; Bhandary, Suman; Das, Sukhen; Bhattacharya, Abhishek; Basu, Ruma; Nandy, Papiya

doi:10.1016/j.msec.2012.05.011

Cited by 61 publications

(42 citation statements)

References 25 publications

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“…[32] Previous reports have pointed out that the generation of intracellular ROS was considered as one of the important factors for the antibacterial activity of silver nanoparticles. [33, 34] 2’,7’- ichlorofluorescin-diacetate (DCFH-DA) probe, which can be oxidized into highly fluorescent DCF by intracellular ROS, was used to indicate the ROS concentration of bacteria on different material samples in our studies. Figure 6 revealed a significant increasing trend of fluorescence intensity produced as an indicator of free radical formation in Ti6Al4V5Cu group compared to the blank control group after 6 h (p < 0.01).…”

Section: Discussionmentioning

confidence: 99%

Toward a Molecular Understanding of the Antibacterial Mechanism of Copper‐Bearing Titanium Alloys against Staphylococcus aureus

Zhu

et al. 2015

Adv Healthcare Materials

140

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The antibacterial mechanism of the Cu-containing materials has not been fully understood although such understanding is crucial for the sustained clinical use of Cu-containing antibacterial materials such as bone implants. The aim of this study is to investigate the molecular mechanisms by which the Gram-positive Staphylococcus aureus (S.aureus) is inactivated through Cu-bearing titanium alloys (Ti6Al4V5Cu). Cu ions released from the alloys were found to contribute to lethal damage of bacteria. They destroyed the permeability of the bacterial membranes, resulting in the leakage of reducing sugars and proteins from the cells. They also promoted the generation of bacteria-killing reactive oxygen species (ROS). The ROS production was confirmed by several assays including fluorescent staining of intracellular oxidative stress, detection of respiratory chain activity, and measurement of the levels of lipid peroxidation, catalase and glutathione. Furthermore, the released Cu ions showed obvious genetic toxicity by interfering the replication of nuc (species-specific) and 16SrRNA genes, but with no effect on the genome integrity. All of these effects lead to the antibacterial effect of Ti6Al4V5Cu. Collectively, our work reconciles the conflicting antibacterial mechanisms of Cu-bearing metallic materials or nanoparticles reported in the literature, as well as highlight the potential use of Ti6Al4V5Cu alloys in inhibiting bacterial infections.

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Section: Discussionmentioning

confidence: 99%

Toward a Molecular Understanding of the Antibacterial Mechanism of Copper‐Bearing Titanium Alloys against Staphylococcus aureus

Zhu

et al. 2015

Adv Healthcare Materials

140

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“…37,117 In experimental animals, the LD 50 reported antimicrobial efficacy and biocompatibility of mullite aggregates impregnated with copper nanoparticles (below 100 mg/mL). 118 Zinc is a vital micronutrient that forms a component of enzymes needed to maintain structural integrity of proteins and regulation of gene expression. It is essential in protein synthesis, T-cell development and acts as anti-oxidant.…”

Section: Natural Polymer/metals Based Composites For Wound Healingmentioning

confidence: 99%

Natural Polymer/Inorganic Material Based Hybrid Scaffolds for Skin Wound Healing

et al. 2015

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Dermal tissue engineering focuses on the restoration of diseased and damaged tissues by using a combination of cells, biomaterials, and bioactive molecules. Inorganic substances like zeolites, clay, mesoporous silica, metals, and metal oxides are advanced materials used in wound healing research. They can improve the structural stability and bioactivity of bio polymeric scaffolds. Zeolites, clays, and mesoporous silica act as suitable carriers for drug delivery and when incorporated within scaffolds, serve as ideal matrices for promoting skin regeneration. This review focuses on various natural polymers/inorganic materials based composite scaffolds used for skin tissue engineering, highlighting their synthesis routes and mode of action by which wound healing is enhanced. Among the different inorganic materials used, the role of zeolites incorporated biocomposites for promoting blood coagulation, antibacterial effect; oxygen delivery to cells and wound healing are discussed in detail. The article thus includes recent attempts to explore the hidden potential of inorganic materials in dermal tissue engineering.

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“…Copper ions released subsequently may bind with DNA molecules and lead to disorder of the helical structure by cross-linking within and between the nucleic acid strands resulting in the lysis of dermatophytic cells [21,22].It has been also reported that copper ions penetrate inside cells also disrupt biochemical processes [23]. The exact mechanism behind is not known and needs to be studied further.…”

Section: Antifungal Susceptibility Assaymentioning

confidence: 99%

Copper oxide nanoparticles: an antidermatophytic agent for Trichophyton spp.

Kumar

Shukla

Pandey

et al. 2015

Nanotechnology Reviews

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In the present investigation, we synthesized copper oxide nanoparticles via precipitation method, characterized by SEM, XRD and evaluated their antidermatophytic properties of by broth microdilution method recommended by CLSI. AbstractCopper oxide is one of the most important transition metal oxides due to its unique properties.It is used in various technological applications such as high critical temperature, superconductors, gas sensors, in photoconductive applications and so on. Recently, it has been used as an antimicrobial agent against various pathogenic bacteria. In the present investigation, we studied the structural and antidermatophytic properties of copper oxide nanoparticles (CuO NPs) synthesized by precipitation technique. Copper sulphate was used as a precursor and sodium hydroxide, reducing agent. Scanning Electron Microscope showed flower shaped CuO NPs and X-ray diffraction (XRD) pattern showed the crystalline nature of CuO NPs. These nanoparticles were evaluated against two prevalent species of dermatophytes viz., Trichophyton rubrum and Trichophyton mentagrophytes by using Broth Microdilution Technique. Further, the nanoparticles activity was also compared with synthetic Sertaconazole. Although better antidermatophytic activity was exhibited by Sertaconazole as compared to nanoparticles, yet being a synthetic Sertaconazole may not be preferred as it shows different adverse effect., T. mentagrophytes is more susceptible to nanoparticles than T. rubrum. A phylogenetic approach was applied for predicting differences in susceptibility of pathogens.

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Antimicrobial efficacy and biocompatibility study of copper nanoparticle adsorbed mullite aggregates

Cited by 61 publications

References 25 publications

Toward a Molecular Understanding of the Antibacterial Mechanism of Copper‐Bearing Titanium Alloys against Staphylococcus aureus

Toward a Molecular Understanding of the Antibacterial Mechanism of Copper‐Bearing Titanium Alloys against Staphylococcus aureus

Natural Polymer/Inorganic Material Based Hybrid Scaffolds for Skin Wound Healing

Copper oxide nanoparticles: an antidermatophytic agent for Trichophyton spp.

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