Antimicrobial effects of zero-valent iron nanoparticles on gram-positive Bacillus strains and gram-negative Escherichia coli strains

Hsueh, Yi-Huang; Tsai, Ping-Han; Lin, Kuen‐Song; Ke, Wan-Ju; Chiang, Chao–Lung

doi:10.1186/s12951-017-0314-1

Cited by 48 publications

(23 citation statements)

References 45 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This effect was more pronounced on Staphylococcus aureus as compared with other bacteria (Table 1) which can be related to their complex cell wall in comparison to the Gram-negative bacteria [79]. Previous reports also suggested higher antimicrobial activity of INPs on Gram-positive bacteria as compared to Gram-negative ones [80]. Several factors can explain the bactericidal mechanisms of metallic oxide NPs.…”

Section: Resultsmentioning

confidence: 82%

Green synthesis of iron-based nanoparticles using Chlorophytum comosum leaf extract: methyl orange dye degradation and antimicrobial properties

Ardakani

Alimardani

Tamaddon

et al. 2021

Heliyon

View full text Add to dashboard Cite

Nowadays, green synthesis methods have gained growing attention in nanotechnology owning to their versatile features including high efficiency, cost-effectiveness, and eco-friendliness. Here, the aqueous extract of Chlorophytum comosum leaf was applied for the preparation of iron nanoparticles (INPs) to obtain spherical and amorphous INPs with a particle size below 100 nm as confirmed by TEM. The synthesized INPs managed to eliminate methyl orange (MO) from the aqueous solution. The concentration of MO can be easily checked via ultraviolet-visible (UV-Vis) spectroscopy throughout the usage of INPs at the presence of H 2 O 2 . The synthesized INPs exhibited MO degradation efficiency of 77% after 6 h. Furthermore, the synthesized INPs exhibited antibacterial activity against both Gram-negative and Gram-positive bacteria. The prepared INPs have an impressive effect on Staphylococcus aureus at concentrations below 6 μg/ml. Overall, the synthesized INPs could considerably contribute to our combat against organic dyes and bacteria.

show abstract

Section: Resultsmentioning

confidence: 82%

Green synthesis of iron-based nanoparticles using Chlorophytum comosum leaf extract: methyl orange dye degradation and antimicrobial properties

Ardakani

Alimardani

Tamaddon

et al. 2021

Heliyon

View full text Add to dashboard Cite

show abstract

“…We attribute this to the high reactivity of zero-valent iron and its ability to increase reactive oxygen species (ROS) in the local region [182]. Although speculative at this stage, it is also possible that high levels of iron contribute to cellular toxicity.…”

Section: Biofilm Inhibition In Liquid Culturesmentioning

confidence: 95%

Antibacterial activity of iron oxide, iron nitride, and tobramycin conjugated nanoparticles against Pseudomonas aeruginosa biofilms

Armijo¹,

Wawrzyniec²,

Kopciuch³

et al. 2020

J Nanobiotechnol

123

View full text Add to dashboard Cite

Background: Novel methods are necessary to reduce morbidity and mortality of patients suffering from infections with Pseudomonas aeruginosa. Being the most common infectious species of the Pseudomonas genus, P. aeruginosa is the primary Gram-negative etiology responsible for nosocomial infections. Due to the ubiquity and high adaptability of this species, an effective universal treatment method for P. aeruginosa infection still eludes investigators, despite the extensive research in this area. Results:We report bacterial inhibition by iron-oxide (nominally magnetite) nanoparticles (NPs) alone, having a mean hydrodynamic diameter of ~ 16 nm, as well as alginate-capped iron-oxide NPs. Alginate capping increased the average hydrodynamic diameter to ~ 230 nm. We also investigated alginate-capped iron-oxide NP-drug conjugates, with a practically unchanged hydrodynamic diameter of ~ 232 nm. Susceptibility and minimum inhibitory concentration (MIC) of the NPs, NP-tobramycin conjugates, and tobramycin alone were determined in the PAO1 bacterial colonies. Investigations into susceptibility using the disk diffusion method were done after 3 days of biofilm growth and after 60 days of growth. MIC of all compounds of interest was determined after 60-days of growth, to ensure thorough establishment of biofilm colonies. Conclusions:Positive inhibition is reported for uncapped and alginate-capped iron-oxide NPs, and the corresponding MICs are presented. We report zero susceptibility to iron-oxide NPs capped with polyethylene glycol, suggesting that the capping agent plays a major role in enabling bactericidal ability in of the nanocomposite. Our findings suggest that the alginate-coated nanocomposites investigated in this study have the potential to overcome the bacterial biofilm barrier. Magnetic field application increases the action, likely via enhanced diffusion of the iron-oxide NPs and NP-drug conjugates through mucin and alginate barriers, which are characteristic of cystic-fibrosis respiratory infections. We demonstrate that iron-oxide NPs coated with alginate, as well as alginate-coated magnetite-tobramycin conjugates inhibit P. aeruginosa growth and biofilm formation in established colonies. We have also determined that susceptibility to tobramycin decreases for longer culture times. However, susceptibility to the iron-oxide NP © The Author(s) 2020. This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article' s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article'

show abstract

“…The reductase activities of S. aureus SA113 and E. coli K12 were determined using a BacLight™ RedoxSensor™ Green Vitality Kit (Thermo Fisher, Waltham, MA, USA) [43,44]. Overnight cultures of approximately 1 × 10 7 CFU/mL in 25 mL of MHB were supplemented with the indicated concentrations of rGO for 3 h at 175 rpm and 37 °C.…”

Section: Methodsmentioning

confidence: 99%

Antibacterial Property of Composites of Reduced Graphene Oxide with Nano-Silver and Zinc Oxide Nanoparticles Synthesized Using a Microwave-Assisted Approach

Hsueh

Hsieh

Chiu

et al. 2019

IJMS

Self Cite

View full text Add to dashboard Cite

Graphene oxide (GO) composites with various metal nanoparticles (NPs) are attracting increasing interest owing to their broad scope in biomedical applications. Here, microwave-assisted chemical reduction was used to deposit nano-silver and zinc oxide NPs (Ag and ZnO NPs) on the surface of reduced GO (rGO) at the following weight percentages: 5.34% Ag/rGO, 7.49% Ag/rGO, 6.85% ZnO/rGO, 16.45% ZnO/rGO, 3.47/34.91% Ag/ZnO/rGO, and 7.08/15.28% Ag/ZnO/rGO. These materials were tested for antibacterial activity, and 3.47/34.91% Ag/ZnO/rGO and 7.08/15.28% Ag/ZnO/rGO exhibited better antibacterial activity than the other tested materials against the gram-negative bacterium Escherichia coli K12. At 1000 ppm, both these Ag/ZnO/rGO composites had better killing properties against both E. coli K12 and the gram-positive bacterium Staphylococcus aureus SA113 than Ag/rGO and ZnO/rGO did. RedoxSensor flow cytometry showed that 3.47/34.91% Ag/ZnO/rGO and 7.08/15.28% Ag/ZnO/rGO decreased reductase activity and affected membrane integrity in the bacteria. At 100 ppm, these two composites affected membrane integrity more in E. coli, while 7.08/15.28% Ag/ZnO/rGO considerably decreased reductase activity in S. aureus. Thus, the 3.47/34.91% and 7.08%/15.28% Ag/ZnO/rGO nanocomposites can be applied not only as antibacterial agents but also in a variety of biomedical materials such as sensors, photothermal therapy, drug delivery, and catalysis, in the future.

show abstract

Antimicrobial effects of zero-valent iron nanoparticles on gram-positive Bacillus strains and gram-negative Escherichia coli strains

Cited by 48 publications

References 45 publications

Green synthesis of iron-based nanoparticles using Chlorophytum comosum leaf extract: methyl orange dye degradation and antimicrobial properties

Green synthesis of iron-based nanoparticles using Chlorophytum comosum leaf extract: methyl orange dye degradation and antimicrobial properties

Antibacterial activity of iron oxide, iron nitride, and tobramycin conjugated nanoparticles against Pseudomonas aeruginosa biofilms

Antibacterial Property of Composites of Reduced Graphene Oxide with Nano-Silver and Zinc Oxide Nanoparticles Synthesized Using a Microwave-Assisted Approach

Contact Info

Product

Resources

About