Antimicrobial effect of emulsion-encapsulated isoeugenol against biofilms of food pathogens and spoilage bacteria

Nielsen, Christina Krogsgård; Kjems, Jørgen; Mygind, Tina; Snabe, Torben; Schwarz, Karin; Serfert, Yvonne

doi:10.1016/j.ijfoodmicro.2016.11.002

Cited by 41 publications

(18 citation statements)

References 24 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The results of the present study revealed an antimicrobial effect of Tween 80 on B. weihenstephanensis KBAB4 spores and on vegetative cells which depended on pH. An effect of Tween 80 on the action of antimicrobial compounds has [Texte] previously been mentioned in the context of interactions in food systems (Castro et al, 2009;Jung et al, 1992;Krogsgard-Nielsen et al, 2017). Tween may have hampered the antimicrobial properties of isoeugenol in an emulsion (Krogsgard-Nielsen et al, 2017).…”

Section: Discussionmentioning

confidence: 63%

“…An effect of Tween 80 on the action of antimicrobial compounds has [Texte] previously been mentioned in the context of interactions in food systems (Castro et al, 2009;Jung et al, 1992;Krogsgard-Nielsen et al, 2017). Tween may have hampered the antimicrobial properties of isoeugenol in an emulsion (Krogsgard-Nielsen et al, 2017). The solubilization capacity of the emulsifier on isoeugenol was so strong that it prevented the antimicrobial compound from penetrating the cell membrane.…”

Section: Discussionmentioning

confidence: 99%

“…According to the scientific literature, Tween 80 is particularly commonly used in food emulsions containing microorganisms and its concentration ranges from 0.1 to 10 % (w/w) (Buranasuksombat, Kwon, Turner, & Bhandari, 2011;Celiktas et al, 2007;Hilbig, Ma, Davidson, Weiss, & Zhong, 2016;Krogsgard-Nielsen et al, 2017;Landry et al, 2016;Murray, 2016;Neves et al, 2016;Ribes, Fuentes, Talens, & Barat, 2017). Both emulsifiers were chosen to stabilize the emulsion because of this non-ionic characteristic to limit artefacts.…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Dispersed phase volume fraction, weak acids and Tween 80 in a model emulsion: Effect on the germination and growth of Bacillus weihenstephanensis KBAB4 spores

Léonard-Akkari

Guégan

Courand

et al. 2018

Food Research International

View full text Add to dashboard Cite

In foodstuffs, physico-chemical interactions and/or physical constraints between spores, inhibitors and food components may exist. Thus, the objective of this study was to investigate such interactions using a model emulsion as a microbial medium in order to improve bacterial spore control with better knowledge of the interactions in the formulation. Emulsions were prepared with hexadecane mixed with nutrient broth using sonication and were stabilized by Tween 80 and Span 80. The hexadecane ratio was either 35% (v/v) or 50% (v/v) and each emulsion was studied in the presence of organic acid (acetic, lactic or hexanoic) at two pH levels (5.5 and 6). Self-diffusion coefficients of emulsion components and the organic acids were measured by Pulsed Field Gradient-Nuclear Magnetic Resonance (PFG-NMR). The inhibition effect on the spore germination and cell growth of Bacillus weihenstephanensis KBAB4 was characterized by the measure of the probability of growth using the most probable number methodology, and the measure of the time taken for the cells to germinate and grow using a single cell Bioscreen® method and using flow cytometry. The inhibition of spore germination and growth in the model emulsion depended on the dispersed phase volume fraction and the pH value. The effect of the dispersed phase volume fraction was due to a combination of (i) the lipophilicity of the biocide, hexanoic acid, that may have had an impact on the distribution of organic acid between hexadecane and the aqueous phases and (ii) the antimicrobial activity of the emulsifier Tween 80 detected at the acidic pH value. The interface phenomena seemed to have a major influence. Future work will focus on the exploration of these phenomena at the interface.

show abstract

Section: Discussionmentioning

confidence: 63%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Dispersed phase volume fraction, weak acids and Tween 80 in a model emulsion: Effect on the germination and growth of Bacillus weihenstephanensis KBAB4 spores

Léonard-Akkari

Guégan

Courand

et al. 2018

Food Research International

View full text Add to dashboard Cite

show abstract

“…Biofilms are communities of microorganisms living at an interphase where they attach to each other through the extracellular polymeric substance also known as the biofilm matrix composed of extracellular DNA, proteins, and polysaccharides. Due to the protection of this matrix, bacteria show up to 1000 times greater tolerance to antibiotics and biocides than their planktonic counterparts (48). Because of this, it is important to find compounds that interfere with the early steps of biofilm formation and slow down its formation rate.…”

Section: Resultsmentioning

confidence: 99%

Evaluation of the antimicrobial activity and cytotoxicity of different components of natural origin present in essential oils

García-Salinas¹,

Elizondo²,

Arruebo

et al. 2018

Preprint

View full text Add to dashboard Cite

23The antimicrobial action of different components present in essential oils including carvacrol, 24 cinnamaldehyde, thymol, squalene, rosmarinic acid, tyrosol, eugenol and -Caryophyllene 25 against Gram-positive and Gram-negative bacteria is here reported. Planktonic bacteria as well 26 as a model of biofilm forming bacteria were challenged against those components being 27 carvacrol, cinnamaldehyde and thymol the components with the highest antimicrobial action 28 in both different settings. The potential synergy of some of those components against 29 pathogenic bacteria was also analyzed. The antimicrobial mechanism of the different 30 components was analyzed by means of flow cytometry and by electronic and confocal 31 microscopy. Finally, subcytotoxic doses against mammalian cell lines are here reported to 32 highlight the reduced cytotoxicity of those components against eukaryotic cells. Carvacrol, 33 cinnamaldehyde and thymol showed the highest antimicrobial action of all the natural origin 34 compounds tested and lower cytotoxicity against eukaryotic cells than conventional antiseptics 35 such as chlorhexidine. The high inhibition in biofilm forming activity of those components 36 highlight also their demonstrate benefits in reducing pathogenic microorganisms. 37 38 Importance 39The use and misuse of antibiotics has led to the emergence of antibiotic resistance to human 40 and animal pathogens. Compounds from natural sources such as animals, plants, and 41 microorganisms have been proposed as renewed potential antimicrobial alternatives. The 42 comparative antimicrobial action of different components commonly present in essential oils 43 including carvacrol, cinnamaldehyde, thymol, squalene, rosmarinic acid, tyrosol, eugenol and 44 -Caryophyllene against S. aureus and E. coli is here reported. Carvacrol, cinnamaldehyde and 45 thymol are the components with the highest antimicrobial action. Bacteria membrane 46 disruption represents the bactericidal mechanism attributable to these compounds. In 47 3 addition, the presence of carvacrol, cinnamaldehyde and thymol hinders S. aureus biofilm 48 formation and partially eliminates preformed biofilms. Those components are less toxic to 49 human cells than chlorhexidine. 50 51 52

show abstract

“…Many studies have focused on the development of micro‐ or nanoparticle‐based systems for increased antimicrobial stability and activity but, to date, information about their potential use in food products is rather limited (Castro‐Rosas et al., ). For instance, encapsulation of antimicrobial compounds has demonstrated to enhance their stability during food‐processing treatments, such as electron beam irradiation (Gomes, Moreira, & Castell‐Perez, ), and the usefulness of these techniques to generate natural additives (Ko, Kim, & Park, ) or to formulate antibacterial disinfectants (Krogsgard Nielsen et al., ). Similarly, encapsulation of antivirals for food applications has been scarcely explored.…”

Section: Development Of Food‐grade Polymers and Biopolymers With Antimentioning

confidence: 99%

Polymers and Biopolymers with Antiviral Activity: Potential Applications for Improving Food Safety

Randazzo

Fabra

Falcó

et al. 2018

Comp Rev Food Sci Food Safe

View full text Add to dashboard Cite

Gastroenteritis and hepatitis, caused by human noroviruses (HuNoVs) and hepatitis A virus (HAV), respectively, are the most common illnesses resulting from the consumption of food contaminated with human enteric viruses. Food-grade polymers can be tailor designed to improve food safety, either as novel food-packaging materials imparting active antimicrobial properties, applied in food contact surfaces to avoid cross-contamination, or as edible coatings to increase fresh produce's shelf life. The incorporation of antimicrobial agents into food-grade polymers can be used to control the food microbiota and even target specific foodborne pathogens to improve microbiological food safety and to enhance food quality. Enteric viruses are responsible for one fifth of acute gastroenteritis cases worldwide and the development of food-grade polymers and biopolymers with antiviral activity for food applications is a topic of increased interest, both for academia and the food industry, even though developments are still limited. This review compiles existing studies in this widely unexplored area and highlights the potential of these developments to improve viral food safety.

show abstract

Antimicrobial effect of emulsion-encapsulated isoeugenol against biofilms of food pathogens and spoilage bacteria

Cited by 41 publications

References 24 publications

Dispersed phase volume fraction, weak acids and Tween 80 in a model emulsion: Effect on the germination and growth of Bacillus weihenstephanensis KBAB4 spores

Dispersed phase volume fraction, weak acids and Tween 80 in a model emulsion: Effect on the germination and growth of Bacillus weihenstephanensis KBAB4 spores

Evaluation of the antimicrobial activity and cytotoxicity of different components of natural origin present in essential oils

Polymers and Biopolymers with Antiviral Activity: Potential Applications for Improving Food Safety

Contact Info

Product

Resources

About