Antimicrobial effect of chlorhexidine on Aggregatibacter actinomycetemcomitans biofilms associated with peri-implantitis

Abstract

Background. This study aimed to assessthe antimicrobial effect of chlorhexidine (CHX) on Aggregatibacter actinomycetemcomitans biofilms isolated from subgingival plaque of peri-implantitis lesions.Methods. Thirteen patients requiring peri-implantitis treatment were consecutively selected and their subgingival biofilm was collected by inserting fine sterile paper points into peri-implant pockets for 15 seconds. A. actinomycetemcomitans was isolated from the subgingival biofilm and cultured. In this study, the standard strain of A. actinomycetemcomitans served as the positive control group and a blank disc impregnated with water served as the negative control; 0.1 mL of the bacterial suspension was cultured on specific culture medium and blank discs (6 mm in diameter) impregnated with 0.2%CHX mouthrinse (Behsa Pharmaceutical Co.) and negative control discs were placed on two sides of the bacterial culture plate. The size of growth inhibition zone was measured by a blinded independent observer in millimetres.Results. According to the results of disc diffusion test, the mean diameter of growth inhibition zone of A. actinomycetemcomitans around discs impregnated with CHX was larger in both standard (positive control) and biofilm samples of A. actinomycetemcomitans compared to the negative control group (blank disc) (P<0.001).Conclusion. Use of0.2% CHX mouthwash had antibacterial effects on A. actinomycetemcomitans species isolated from peri-implantitis sites.

“Inhibitory effect of Brazilian red propolis on Candida biofilms developed on titanium surfaces”

“…In fact, antimicrobial mouthwash solutions are the most widespread, because it is an easy and cheap method [11]. Chlorhexidine 0.12% is considered the chemical gold standard for the treatment of peri-implantitis [12]. Although its use is recommended, using the therapy for a prolonged period of time has adverse effects, such as desquamated lesions, teeth and mucosal staining, loss of taste and dry mouth [13].…”

Low‐temperature plasma on peri‐implant–related biofilm and gingival tissue

“…The results did not show any difference between GAS and NEGATIVE treatments, meaning that biofilm inactivation was promoted by plasma and not by the gas or the mechanical influence. For the gold standard treatment for anti‐plaque therapy, chlorhexidine digluconate (CHX 0.2%) was used as a positive control, considering it shows antibacterial effects against periodontopathogens …”

“…For the gold standard treatment for anti-plaque therapy, chlorhexidine digluconate (CHX 0.2%) was used as a positive control, considering it shows antibacterial effects against periodontopathogens. [17][18][19][20] Previous studies have shown the antimicrobial effect of LTP application on Streptococcus sanguinis, C. albicans, and S. aureus biofilm, and S. Mitis. 11,12,21 A study conducted in beagles also showed a significant decrease in detection of bacteria (P. gingivalis and Tannerella forsythia) when nonequilibrium plasma treatment was applied as an adjunct to the conventional therapy.…”

Effect of Platform Switched Implants on Supporting Structures of Mandibular Overdentures

“…Studies have shown that bacterial contamination of the microcracks between the implant and the abutment adversely affects the stability of the peri-implant tissues. (7)(8)(9) In an attempt to improve long-term bone maintenance around implants, implant-to abutment connection referred to as "platform switching" has been proposed. It refers to the use of an abutment of smaller diameter connected to implant neck of larger diameter.…”