Antimicrobial Effect of Aqueous, Ethanol, Methanol and Glycerin Extracts of Satureja bachtiarica on Streptococcus pyogenes, Pseudomonas aeruginosa and Staphylococcus epidermidis

Abstract

Background:The Iranian medicinal plants, such as Satureja bachtiarica have been utilized as traditional medicines by the indigenous people of Chaharmahal and Bakhtiari in Iran. Objectives: According to biologically active compounds and traditional use of the Satureja bachtiarica, seem that this plant has significant antimicrobial effects. Materials and Methods: In this experimental study, Satureja bachtiarica after extraction with watery, ethanol 96%, methanol 96% and 20% glycerin antimicrobial effect of extract were determined by "screening antimicrobial activity" and "disk agar diffusion test" in 10, 20, 30 and 40 mg/mL concentration of the extract against Streptococcus pyogenes PTCC 1447, Pseudomonas aeruginosa PTCC 1310 and Staphylococcus epidermidis PTCC 1435. Statistical analysis was carried out by analysis of variance (ANOVA). Results:The results showed that all extracts were quite effective in 2 mg/mL concentration on S. pyogenes and S. epidermidis and were prevented from growth them on medium, while extracts have no certain antimicrobial effect on P. aeruginosa. In "disk agar diffusion method", 10, 20, 30 and 40 mg/mL aqueous, ethanol 96%, methanol 96% and 20% glycerin extract concentrations, was inhibited effect on S. pyogenes and S. epidermidis, but 40 mg/mL aqueous and 30 and 40 mg/mL ethanol, methanol and glycerin extract concentrations, has inhibited effect on P. aeruginosa prevent them growing. The results indicate that alcoholic and aqueous extracts of S. bachtiarica have the greatest effect on gram positive bacterium S. pyogenes. Conclusions: As a result aqueous and alcoholic extracts of S. bachtiarica, have been strong antimicrobial activity against many food pathogen bacteria.

Application of intelligent modeling to predict the population dynamics of Pseudomonas aeruginosa in Frankfurter sausage containing Satureja bachtiarica extracts

“…Heidari Sureshjani et al (2013) also reported higher inhibitory and bactericidal activities for ethanol extract of Satureja than those of the water extract. They attributed these findings to the dry weight of the extract [12].…”

“…They reported that the antimicrobial effect increased as the concentrations increased because the antimicrobial compounds increased [12]. It has been shown in several studies that different concentrations of the extract influence the increment of antimicrobial effect.…”

“…Satureja bachtiarica is widely scattered in Iran and mainly found in Chaharmahaland Bakhtiari Province, Lorestan, Khouzestan, Ilam, Kermanshah, Isfahan, Gilan and other regions. It has rectangular leaves, folded in length, which are integrated around the stem [12].…”

“…Few researches have been conducted on the antimicrobial effect of Satureja extract on P. aeruginosa in a complex food system such as sausage [12]. This paper, thus, aimed at the study of the effect of time, extract type, extract concentration and ambient temperature on the population dynamics of P. aeruginosa in a complex food system (Frankfurter sausage) using stepwise regression, genetic algorithm-artificial neural networks (GA-ANN) and CANFIS.…”

Antimicrobial, antioxidant, cytotoxic and apoptotic activities of Satureja khuzestanica

“…Aromatic plants, known for both aromatic and antiseptic properties, are used in spices and natural food preservatives, perfume industry for aromatherapy, etc. (2,3). During the last decades, natural products with antimicrobial effect have been investigated for eliminating the use of synthetic antibiotics that cause the resistance of microorganisms and have side effects to human health (4).…”