Antimicrobial constituents of Angelica dahurica roots

Kwon, Yong Soo; Kobayashi, Akio; Kajiyama, Shin Ichiro; Kawazu, Kazuyoshi; Kanzaki, Hiroshi; Kim, Chang Min

doi:10.1016/s0031-9422(96)00634-6

Cited by 103 publications

(64 citation statements)

References 7 publications

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“…It could be supposed that flavonoids might be responsible for this activity. This is supported by the overall poor activity shown by pure coumarins (22), although in earlier studies some antibacterial inhibitory effects have been found for xanthotoxin, herniarin, umbelliferone and scopoletin, and antifungal activity for umbelliferone, scopoletin and coumarin (9,10,12,16). Further research will be focused on the identification and antimicrobial activity of the isolated metabolites of this plant.…”

Section: Antimicrobial Activitymentioning

confidence: 81%

Phytochemical and Antimicrobial Screening of Leaves and Stems of Balkan Endemic SpeciesDaphne MalyanaBlečić

Jušković

Vasiljević

Manojlović

et al. 2012

Biotechnology & Biotechnological Equipment

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Section: Antimicrobial Activitymentioning

confidence: 81%

Phytochemical and Antimicrobial Screening of Leaves and Stems of Balkan Endemic SpeciesDaphne MalyanaBlečić

Jušković

Vasiljević

Manojlović

et al. 2012

Biotechnology & Biotechnological Equipment

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“…1), a plant-derived component, exists widely in the Umbelliferae, and includes Angelica dahurica, Notopterygium incisum, Angelica pubescens and Peucedanum praeruptorum. Isoimperatorin has analgesic (18), antimicrobial (19), vascular relaxing (20) and anticancer activities (21)(22)(23). However, studies by Okuyama et al (21), Zhang (22) and Kim et al (23) focused predominantly on investigating the antiproliferative activity of isoimperatorin against different cancer cell lines using MTT or sulforhodamine B techniques.…”

Section: Introductionmentioning

confidence: 99%

Isoimperatorin induces apoptosis of the SGC-7901 human gastric cancer cell line via the mitochondria-mediated pathway

2016

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Abstract. The present study was designed to investigate the antiproliferative activity of isoimperatorin against SGC-7901 cells and to examine the possible mechanisms. The antiproliferative activity of isoimperatorin against SGC-7901 cells was evaluated using an MTT assay, and the mechanisms were investigated using flow cytometry and western blot assays, which were used to determine the apoptotic rate and expression levels of mitochondria-mediated apoptosis-associated proteins, including Survivin, myeloid leukemia cell-1 (Mcl-1), B cell lymphoma-extra large (Bcl-xl), B cell lymphoma 2 (Bcl-2), second mitochondria-derived activator of caspase (Smac), Bcl-2-associated X factor (Bax), cleaved (c)-caspase-3 and c-caspase-9 in SGC-7901 cells. Additionally, a xenograft assay was used to confirm whether isoimperatorin had an inhibitory effect on SGC-7901 cell-induced tumors in vivo. The results of the MTT assay suggested that isoimperatorin significantly inhibited the proliferation of SGC-7901 cells in a dose-and time-dependent manner, and the half maximal inhibitory concentration was 18.75 µg/ml. The results of the flow cytometric analysis indicated that, following treatment with isoimperatorin, the apoptotic rate of SGC-7901 cells was significantly increased, compared with that of cells in the control group. The results of the western blot analysis indicated that, following treatment with isoimperatorin, the expression levels of the pro-apoptotic proteins, Bax, c-caspase-3 and c-caspase-9, were significantly increased and the expression levels of the anti-apoptotic proteins, Survivin and Bcl-2, were significantly reduced, compared with the control group. No alterations in expression were found in the other apoptosis-associated proteins, including Mcl-1, Bcl-xl and Smac. The results of the xenograft assay indicated that isoimperatorin significantly inhibited the growth of SGC-7901 cell-induced tumor in vivo by increasing the expression levels of pro-apoptotic proteins (Bax, c-caspase-3 and c-caspase-9) and reducing the expression levels of anti-apoptotic proteins (Survivin and Bcl-2) without adverse effects on the increasing body weight of nude mice. In conclusion, the present study revealed that isoimperatorin may be able to induce the apoptosis of SGC-7901 cells in vitro and in vivo by regulating the expression levels of mitochondria-mediated apoptosis-associated proteins.

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“…ADR contains effective components such as coumarins and terpenoid (Kim et al, 1998;Joo and Kang, 2005). Its some coumarins on ADR are reported to have anti-microbial, oxidative, and thrombotic activity Kwon et al, 1997;Ryu et al, 2001;Lechner et al, 2004, Song et al, 2005. Meanwhile, inflammation is associated with the various diseases and other studies on anti-inflammatory activity have reported in medical plants (Shin et al, 1988;Son et al, 2007).…”

Section: Introductionmentioning

confidence: 99%

Inhibitory Effects of Angelicae Dahuricae Radix Extract on COPD induced by Cigarette Smoke Condensate and Lipopolysaccharide in Mice

Kwak¹,

Lim²

2011

Korean Journal of Medicinal Crop Science

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show abstract

Antimicrobial constituents of Angelica dahurica roots

Cited by 103 publications

References 7 publications

Phytochemical and Antimicrobial Screening of Leaves and Stems of Balkan Endemic SpeciesDaphne MalyanaBlečić

Phytochemical and Antimicrobial Screening of Leaves and Stems of Balkan Endemic SpeciesDaphne MalyanaBlečić

Isoimperatorin induces apoptosis of the SGC-7901 human gastric cancer cell line via the mitochondria-mediated pathway

Inhibitory Effects of Angelicae Dahuricae Radix Extract on COPD induced by Cigarette Smoke Condensate and Lipopolysaccharide in Mice

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