Antimicrobial assay of endophytic fungi from Rumex madaio and chemical study of strain R1

X, Bai; Yu, Ronglu; Li, Mingzhu; Zhang, Huawei

doi:10.3329/bjp.v14i3.41598

Cited by 4 publications

(3 citation statements)

References 27 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The endophytic strain R1 was isolated and purified from the coastal plant Rumex madaio Makino, collected off Putuo Island (Zhoushan, China) [43]. A suspension of culture containing its mycelia in PDA supplemented with glycerol (20% v/v) was stored at −80 • C at Zhejiang University of Technology (Hangzhou, China).…”

Section: Microbes and Cultivationmentioning

confidence: 99%

Genome Features and AntiSMASH Analysis of an Endophytic Strain Fusarium sp. R1

Liu

Tang

et al. 2022

Metabolites

Self Cite

View full text Add to dashboard Cite

Endophytic fungi are one of the most prolific sources of functional biomolecules with therapeutic potential. Besides playing an important role in serious plant diseases, Fusarium strains possess the powerful capability to produce a diverse array of bioactive secondary metabolites (SMs). In order to in-depth mine gene clusters for SM biosynthesis of the genus Fusarium, an endophytic strain Fusarium sp. R1 isolated from Rumex madaio Makino was extensively investigated by whole-genome sequencing and in-depth bioinformatic analysis, as well as antiSMASH annotation. The results displayed that strain R1 harbors a total of 51.8 Mb genome, which consists of 542 contigs with an N50 scaffold length of 3.21 Mb and 50.4% GC content. Meanwhile, 19,333 functional protein-coding genes, 338 tRNA and 111 rRNA were comprehensively predicted and highly annotated using various BLAST databases including non-redundant (Nr) protein sequence, nucleotide (Nt) sequence, Swiss-Prot, Gene Ontology (GO), Kyoto Encyclopedia of Genes and Genomes (KEGG) and Clusters of Orthologous Groups (COG), as well as Pathogen Host Interactions (PHI) and Carbohydrate-Active enzymes (CAZy) databases. Antibiotics and Secondary Metabolites Analysis Shell (AntiSMASH) results showed that strain R1 has 37 SM biosynthetic gene clusters (BGCs), including 17 nonribosomal peptide synthetases (NRPSs), 13 polyketide synthetases (PKSs), 3 terpene synthases (Ts), 3 hybrid NRPS + PKS and 1 hybrid indole + NRPS. These findings improve our knowledge of the molecular biology of the genus Fusarium and would promote the discovery of new bioactive SMs from strain R1 using gene mining strategies including gene knockout and heteroexpression.

show abstract

Section: Microbes and Cultivationmentioning

confidence: 99%

Genome Features and AntiSMASH Analysis of an Endophytic Strain Fusarium sp. R1

Liu

Tang

et al. 2022

Metabolites

Self Cite

View full text Add to dashboard Cite

show abstract

“…Endophytes are also known to possess the ability to produce metabolites similar to those of their host plants, exhibiting comparable bioactivity. Since the isolation of endophytic fungus producing paclitaxel from Taxus brevifolia by Stierle in 1993 [ 14 ], numerous NPs with potent bioactivities have been discovered [ 15 – 17 ], fueling global interest among researchers in exploring bioactive substances derived from endophytic microorganisms and positioning it as a leading area of innovation in drug development.…”

Section: Introductionmentioning

confidence: 99%

EMNPD: a comprehensive endophytic microorganism natural products database for prompt the discovery of new bioactive substances

Xu,

Xiao,

et al. 2023

J Cheminform

View full text Add to dashboard Cite

The discovery and utilization of natural products derived from endophytic microorganisms have garnered significant attention in pharmaceutical research. While remarkable progress has been made in this field each year, the absence of dedicated open-access databases for endophytic microorganism natural products research is evident. To address the increasing demand for mining and sharing of data resources related to endophytic microorganism natural products, this study introduces EMNPD, a comprehensive endophytic microorganism natural products database comprising manually curated data. Currently, EMNPD offers 6632 natural products from 1017 endophytic microorganisms, targeting 1286 entities (including 94 proteins, 282 cell lines, and 910 species) with 91 diverse bioactivities. It encompasses the physico-chemical properties of natural products, ADMET information, quantitative activity data with their potency, natural products contents with diverse fermentation conditions, systematic taxonomy, and links to various well-established databases. EMNPD aims to function as an open-access knowledge repository for the study of endophytic microorganisms and their natural products, thereby facilitating drug discovery research and exploration of bioactive substances. The database can be accessed at http://emnpd.idrblab.cn/ without the need for registration, enabling researchers to freely download the data. EMNPD is expected to become a valuable resource in the field of endophytic microorganism natural products and contribute to future drug development endeavors.

show abstract

“…Among 16 fungal strains isolated from the Erigeron canadensis, the ethyl acetate extract of strains NPR003 and NPR005 showed the strongest antifungal inhibitory effects (Bai et al, 2017). Bai et al (2019) isolated six endophytic fungal strains from Rumexmadaio, strains L1 and R1 belonged to the Colletotrichum and Fusarium genera respectively had stronger inhibitory effects on Staphylococcus aureus, and the R1 isolate was the source of two known antimicrobial agents dibutylphthalate and beauvericin. Another known metabolite named resorcinol type of lipid (compound 1) was purified from extract of the endophytic fungus Chaetomium cupreum isolated from the ornamental plant Mussaenda luteola.…”

Section: Introductionmentioning

confidence: 99%

Antimicrobial activity of secondary metabolites produced by Aspergillus neobridgeri isolated from Pistacia lentiscus against multi-drug resistant bacteria

Sadrati¹,

Zerroug²,

Demirel³

et al. 2020

Bangladesh J Pharmacol

View full text Add to dashboard Cite

The in vitro antibacterial activity of the endophytic fungus Aspergillus neobridgeri PLR9 (isolated from the plant Pistacia lentiscus) against a wide range of tested bacteria were studied. The maximum inhibitory activity was found on malt extract agar medium with a mean inhibitory zone of 11.3 mm (p<0.05). The ethyl acetate extract showed a significant inhibitory zone of 13.4 mm (p<0.05), followed by dichloromethane 9.1 mm. Whereas, n-hexane extract showed no activity. The largest inhibition zones were observed against Staphylococcus aureus 3, S. aureus 5 and Escherichia coli 1 with 21.0 mm of diameter. The MIC's were ranged between 312.5 and 1,250 μg/mL. The MBC's were ≤2500 μg/mL for most Gram positive bacteria and ≥2500 μg/mL for most Gram negative bacteria. Time kill assay study against the Enterococcus faecium showed a bactericidal effect at 4x MIC and bacteriostatic against Acinetobacter baumannii at different concentrations. Moreover, it is the first report, PLR9 isolated as endophyte and studied for their antimicrobial activity.

show abstract

Antimicrobial assay of endophytic fungi from Rumex madaio and chemical study of strain R1

Cited by 4 publications

References 27 publications

Genome Features and AntiSMASH Analysis of an Endophytic Strain Fusarium sp. R1

Genome Features and AntiSMASH Analysis of an Endophytic Strain Fusarium sp. R1

EMNPD: a comprehensive endophytic microorganism natural products database for prompt the discovery of new bioactive substances

Antimicrobial activity of secondary metabolites produced by Aspergillus neobridgeri isolated from Pistacia lentiscus against multi-drug resistant bacteria

Contact Info

Product

Resources

About