Antimicrobial activity of secondary metabolites produced by Aspergillus neobridgeri isolated from Pistacia lentiscus against multi-drug resistant bacteria

Sadrati, Nouari; Zerroug, Amina; Demirel, Rasime; Bakli, Sabrina; Harzallah, Daoud

doi:10.3329/bjp.v15i3.40923

Cited by 9 publications

(9 citation statements)

References 48 publications

(57 reference statements)

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“…The plates were refrigerated overnight at 4 °C to facilitate diffusion of metabolites, followed by 16–18 hrs incubation at 37 °C for the bacterial growth. The potential fungal isolates were selected based on the zone of inhibition observed against the test pathogens for secondary screening ( Sadrati et al, 2020 ).…”

Section: Methodsmentioning

confidence: 99%

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Endophytic fungi of Tradescantia pallida mediated targeting of Multi-Drug resistant human pathogens

Dhevi V. Sundar,

Arunachalam

2024

Saudi Journal of Biological Sciences

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Section: Methodsmentioning

confidence: 99%

“…To validate the effectiveness and the outcome of whether ethyl acetate extract is bactericidal or bacteriostatic, MIC index was assessed by dividing MBC by MIC value. If the index is lesser than or equal to 4, then it is considered bactericidal and bacteriostatic if it is greater than 4 ( Sadrati et al, 2020 ).…”

Section: Methodsmentioning

confidence: 99%

Endophytic fungi of Tradescantia pallida mediated targeting of Multi-Drug resistant human pathogens

Dhevi V. Sundar,

Arunachalam

2024

Saudi Journal of Biological Sciences

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“…The MIC index was calculated by dividing MBC by the MIC value. If the index value is less than or equal to 4, it is called bactericidal, and if it is larger than 4, it is considered bacteriostatic (Sadrati et al, 2020).…”

Section: Mic Index Of Fungal Extractmentioning

confidence: 99%

Xenomyrothecium tongaense PTS8: a rare endophyte of Polianthes tuberosa with salient antagonism against multidrug-resistant pathogens

Sundar,

Arunachalam

2024

Front. Microbiol.

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IntroductionEndophytes refer to microorganisms residing within the endosphere of plants, particularly perennials, without inflicting noticeable injury or inducing obvious morphological variations to their host plant or host organism. Endophytic fungi, although often overlooked microorganisms, have garnered interest due to their significant biological diversity and ability to produce novel pharmacological substances.MethodsIn this study, fourteen endophytic fungi retrieved were from the stem of the perennial plant Polianthes tuberosa of the Asparagaceae family. These fungal crude metabolites were tested for antagonistic susceptibility to Multi-Drug Resistant (MDR) pathogens using agar well diffusion, Minimum Inhibitory Concentration (MIC), and Minimum Bactericidal Concentration (MBC) assays. The chequerboard test was used to assess the synergistic impact of active extract.Results and discussionIn early antibacterial screening using the Agar plug diffusion test, three of fourteen endophytes demonstrated antagonism against Methicillin-resistant Staphylococcus aureus (MRSA) and Vancomycin-resistant Enterococcus (VRE). Three isolates were grown in liquid medium and their secondary metabolites were recovered using various organic solvents. Eight extracts from three endophytic fungi displayed antagonism against one or more human pathogens with diameters ranging from 11 to 24 mm. The highest antagonistic effect was obtained in ethyl acetate extract for PTS8 isolate against two MRSA (ATCC 43300, 700699) with 20 ± 0.27 and 22 ± 0.47 mm zones of inhibition, respectively, among different solvent extracts. The extract had MICs of 3.12 ± 0.05 and 1.56 ± 0.05 μg/mL, and MBCs of 50 ± 0.01 and 12.5 ± 0.04 μg/mL, respectively. Antagonism against VRE was 18 ± 0.23 mm Zone of Inhibition (ZOI) with MIC and MBC of 6.25 ± 0.25 and 25 ± 0.01 μg/mL. When ethyl acetate extract was coupled with antibiotics, the chequerboard assay demonstrated a synergistic impact against MDR bacteria. In an antioxidant test, it had an inhibitory impact of 87 ± 0.5% and 88.5 ± 0.5% in 2,2-Diphenyl-1-Picrylhydrazyl and reducing power assay, respectively, at 150 μg/mL concentration. PTS8 was identified as a Xenomyrothecium tongaense strain by 18S rRNA internal transcribed spacer (ITS) sequencing. To our insight, it is the foremost study to demonstrate the presence of an X. tongaense endophyte in the stem of P. tuberosa and the first report to study the antibacterial efficacy of X. tongaense which might serve as a powerful antibacterial source against antibiotic-resistant human infections.

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“…To isolate endophytic fungi; surface sterilization was carried out according to the protocol of Sadrati et al (2020). Briefly, after rinsing with normal tap water to remove the adherent surface, the leaves, stems, and roots were dipped sequentially into 70% ethanol for 1 min, 2% sodium hypochlorite for 3 min, 70% ethanol for 30 sec, and then rinsed with sterilized distilled water three times.…”

Section: Isolation Of Endophytic Fungimentioning

confidence: 99%

Endophytic fungi isolated from Thymus algeriensis with good antimicrobial activities

Nouari

2022

Turkish JAF Sci.Tech.

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The aim of this study was to identify the endophytic fungi associated with medicinal plant Thymus algeriensis and to evaluate their potential for antimicrobial activities. A total of 16 fungi belonging to 6 genera were successfully isolated and identified. The colonization rates ranged from 14.29% to 42.86% and were significantly higher in the roots followed by stems and leaves. Regarding the isolation rates, they were 0.23, 0.14, and 0.1 for the roots, stems, and leaves respectively. Based on the comparison of the morphological characteristics, six genera were identified: Rhizopus, Fusarium, Aspergillus, Alternaria, Phoma, and Penicillium. Fusarium, Phoma, and Alternaria were the most dominant with relative frequencies of 35.5, 28.6, and 14.3% respectively. The fungal endophytes were assessed for their antimicrobial activities using agar plug diffusion method, the best zones of inhibition obtained with the most active endophytic isolates were 20.33 and 20 mm for Fusarium sp. 3, 22.33 and 18.67 mm for Fusarium sp. 5, 23.33 and 25.33 mm for Fusarium sp. 2, and 29.33 and 23 mm for Phoma sp. 4 obtained against Bacillus cereus ATCC 10876 and Staphylococcus aureus ATCC 25923 respectively. The comparison of the averages of inhibition zones obtained against all the pathogenic bacteria showed that the isolates Fusarium sp.3 and Fusarium sp.5 were the most active with mean zones of inhibition of 19.61 and 19.56 mm respectively, followed by Fusarium sp.2 (19 mm) and Phoma sp. 4 (18.61mm). Regarding the antifungal activity, the results showed that the highest inhibition percentages were 60 and 58% obtained by Rhizopus sp. and 51 and 53% obtained by Aspergillus sp. against Fusarium oxysporum f.p. ciccri and Phytophthora infestans respectively. The study concludes the presence of endophytic fungi such as Fusarium, Phoma, Penicillium and Aspergillus associated with Thymus algeriensis that exhibited antibacterial activity. These isolates could serve as potential sources for the isolation of novel antimicrobial agents that may contribute to antibiotic control of antibiotic-resistant bacteria.

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Antimicrobial activity of secondary metabolites produced by Aspergillus neobridgeri isolated from Pistacia lentiscus against multi-drug resistant bacteria

Cited by 9 publications

References 48 publications

Endophytic fungi of Tradescantia pallida mediated targeting of Multi-Drug resistant human pathogens

Endophytic fungi of Tradescantia pallida mediated targeting of Multi-Drug resistant human pathogens

Xenomyrothecium tongaense PTS8: a rare endophyte of Polianthes tuberosa with salient antagonism against multidrug-resistant pathogens

Endophytic fungi isolated from Thymus algeriensis with good antimicrobial activities

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