Antimicrobial and Innate Immune Tolerance Mechanisms in Biofilms

Sandvik, Elizabeth L.; Borgogna, Timothy R.; Stewart, Philip S.

doi:10.1007/978-3-031-10992-8_2

“…Biofilm matrices are composed of biomolecules such as extracellular polysaccharides, nucleic acids, secreted proteins, and lipoproteins [ 56 ] and provide protection for the biofilm communities from environmental stress (pH, osmolality, fluid shear, antibiotics, etc. [ 57 ] or host immune cells [ 58 ]. It allows reduced drug susceptibility and microbial persistence that can contribute to long-term microbial adaptation and genomic modification over generations [ 54 , 59 , 60 ].…”

Section: Impact To Astronaut Health and Other Considerationsmentioning

confidence: 99%

Mitigation and use of biofilms in space for the benefit of human space exploration

Justiniano

¹

,

Goeres

²

,

Sandvik

³

et al. 2023

Biofilm

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“…The ability of microorganisms in biofilms to withstand aggressive antimicrobial treatments has been long recognized [1,2,3,4,5]. The mechanisms behind this recalcitrance are multifaceted and may include delayed or incomplete diffusive penetration [6,7,8], dormancy or persister cell formation [9,10,11], protection by extracellular polymeric substances [12,13,14,15], and active adaptive responses [16,17,18,19,20].…”

Section: Introductionmentioning

confidence: 99%

Simulation of Catalase-Dependent Tolerance of Microbial Biofilm to Hydrogen Peroxide with a Biofilm Computer Model

Stewart

¹

,

Owkes

²

2023

Preprint

1

0

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Hydrogen peroxide (HP) is a common disinfectant and antiseptic. When applied to a biofilm, it may be expected that the top layer of the biofilm would be killed by HP, the HP would penetrate further, and eventually eradicate the entire biofilm. However, using the Biofilm.jl computer model, we demonstrate a mechanism by which the biofilm can persist, and even become thicker, in the indefinite treatment with an HP solution at concentrations that are lethal to planktonic microorganisms. This surprising result is found to be dependent on the neutralization of HP by dead biomass, which provides protection for living biomass deeper within the biofilm. Practically, to control a biofilm, this result leads to the concept of treating with an HP dose exceeding a critical threshold concentration rather than a sustained, lower-concentration treatment.

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Simulation of catalase-dependent tolerance of microbial biofilm to hydrogen peroxide with a biofilm computer model

Stewart,

Owkes

2023

npj Biofilms Microbiomes

Self Cite

0

View full text Add to dashboard Cite

Hydrogen peroxide (HP) is a common disinfectant and antiseptic. When applied to a biofilm, it may be expected that the top layer of the biofilm would be killed by HP, the HP would penetrate further, and eventually eradicate the entire biofilm. However, using the Biofilm.jl computer model, we demonstrate a mechanism by which the biofilm can persist, and even become thicker, in the indefinite treatment with an HP solution at concentrations that are lethal to planktonic microorganisms. This surprising result is found to be dependent on the neutralization of HP by dead biomass, which provides protection for living biomass deeper within the biofilm. Practically, to control a biofilm, this result leads to the concept of treating with an HP dose exceeding a critical threshold concentration rather than a sustained, lower-concentration treatment.

show abstract

Antimicrobial and Innate Immune Tolerance Mechanisms in Biofilms

Cited by 3 publications

References 111 publications

Mitigation and use of biofilms in space for the benefit of human space exploration

Mitigation and use of biofilms in space for the benefit of human space exploration

Simulation of Catalase-Dependent Tolerance of Microbial Biofilm to Hydrogen Peroxide with a Biofilm Computer Model

Simulation of catalase-dependent tolerance of microbial biofilm to hydrogen peroxide with a biofilm computer model

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