Antimicrobial and in vitro wound healing properties of novel clay based bionanocomposite films

Abstract

The present study investigates the development of methyl cellulose (MC)-sodium alginate (SA)-montmorillonite (MMT) clay based bionanocomposite films with interesting wound healing properties. The differential scanning calorimetry analysis of the composite films revealed presence of single glass transition temperature (Tg) confirming the miscible nature of the ternary blended films. The increase in MMT ratio in the composite films reduced the mobility of biopolymer chains (MC/SA) which increased the Tg of the film. Thermogravimetric analysis showed that dispersion of clay (MMT) at nano level significantly delayed the weight loss that correlated with higher thermal stability of the composite films. It was observed that the developed films were able to exhibit antimicrobial activity against four typical pathogenic bacteria found in the presence of wound. The developed films were able to significantly inhibit (10 mg/ml) the growth of Enterococcus faecium and Pseudomonas aeruginosa. In vitro scratch assay indicated potential wound closure activities of MC-2-4 bionanocomposite films at their respective highest subtoxic doses. In conclusion, these ternary bionanocomposite films were found to be promising systems for wound healing applications.

A research of colloidal silver immobilization in bionanocomposites of natural polymers and montmorillonite

“…Con- sequently, the observed decrease in the swelling ability of bionanocomposites, containing colloidal particles of Ag-Mt, can be considered as the superposition of two of these effects. Similar results were obtained in the work [17] when studying the kinetics of swelling of bionanocomposites of chitosan, Na-Mt and Ag-Mt and the kinetics of release of Ag + ions from these bionanocomposites. In the study of water uptake of films of chitosan and its composition with Na-Mt and Ag-Mt, it is found that chitosan composite comprising 10 % Na-Mt has the highest water absorption.…”

“…Comparing these data with the kinetics of swelling of the considered BNCs in water (Fig. 4), it can be noted that the better the BNCs swells in water, the faster the Ag + ions are released from it, as found in [17].…”

Wound Healing Activity of Nanoclay/Spring Water Hydrogels

“…Results of scratch-induced wound healing assays reported by these authors demonstrated that clay mineral particles significantly delayed the gap closure in comparison with control experiments. For instance, while the control showed full gap closure within 24 h, palygorskite showed a mean gap closure of 59 ± 3% after 24 h. Polymeric composite films containing montmorillonite were evaluated by Salcedo et al [ 50 ] and Mishra et al [ 117 ]. The scratch assay showed, in both cases, that the presence of montmorillonite particles alone was able to alter cell behavior (Caco-2 and fibroblasts, respectively for each study), slowing down the gap closure.…”

Effect of the bentonite filler on structure and properties of composites based on hydroxyethyl cellulose

“…It is likely that one of the reasons of microorganisms inactivation is the MMT can adsorb pathogenic bacteria, and this will provides a beneficial physical barrier against them (Mishra et al, 2014). It should be noted that dynamics of the microorganisms inactivation persists during a month.…”

“…Composites of natural biopolymers with aluminosilicates are used as packaging materials in the food industry (Tunç and Duman, 2011). Furthermore, clays are known to exhibit biological activity (Mishra et al, 2014); hence, biopolymers modified by bentonite can be used to design antimicrobial drugs and matrices for drug delivery. But biological activity of HOEC/bentonite composites is poorly understood, although it is important for medical applications.…”