Antimicrobial and cytotoxic activities of indole alkaloids and other constituents from the stem barks of <i>Rauvolfia caffra</i> Sond (Apocynaceae)

Bitombo, André Néhémie; Zintchem, Auguste Abouem A; Atchadé, Alex de Théodore; Ndedi, Esther Del Florence Moni; Khan, Afsar; Bikobo, Dominique Serge Ngono; Pegnyemb, Dieudonné Emmanuel; Bochet, Christian G.

doi:10.1080/14786419.2021.1891054

Cited by 10 publications

(2 citation statements)

References 41 publications

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“…Our study also supports a report by Tlhapi et al, [ 33 ] which identified and isolated raucaffricine from R. caffra extracts. Extracts constituting alkaloids have previously been investigated for pharmacological activities and have been discovered to exhibit potent antimicrobial efficacy [ 10 , 34 ].…”

Section: Discussionmentioning

confidence: 99%

In-silico and in-vitro assessments of some fabaceae, rhamnaceae, apocynaceae, and anacardiaceae species against Mycobacterium tuberculosis H37Rv and triple-negative breast cancer cells

Nyambo

Adu-Amankwaah

Tapfuma

et al. 2023

BMC Complement Med Ther

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Medicinal plants play a huge role in the treatment of various diseases in the Limpopo province (South Africa). Traditionally, concoctions used for treating tuberculosis and cancer are sometimes prepared from plant parts naturally occurring in the region, these include (but not limited to) Schotia brachypetala, Rauvolfia caffra, Schinus molle, Ziziphus mucronate, and Senna petersiana. In this study, the aim was to evaluate the potential antimycobacterial activity of the five medicinal plants against Mycobacterium smegmatis mc2155, Mycobacterium aurum A + , and Mycobacterium tuberculosis H37Rv, and cytotoxic activity against MDA-MB 231 triple-negative breast cancer cells. Phytochemical constituents present in R. caffra and S. molle were tentatively identified by LC-QTOF-MS/MS as these extracts showed antimycobacterial and cytotoxic activity. A rigorous Virtual Screening Workflow (VSW) of the tentatively identified phytocompounds was then employed to identify potential inhibitor/s of M. tuberculosis pantothenate kinase (PanK). Molecular dynamics simulations and post-MM-GBSA free energy calculations were used to determine the potential mode of action and selectivity of selected phytocompounds. The results showed that plant crude extracts generally exhibited poor antimycobacterial activity, except for R. caffra and S. molle which exhibited average efficacy against M. tuberculosis H37Rv with minimum inhibitory concentrations between 0.25–0.125 mg/mL. Only one compound with a favourable ADME profile, namely, norajmaline was returned from the VSW. Norajmaline exhibited a docking score of –7.47 kcal/mol, while, pre-MM-GBSA calculation revealed binding free energy to be –37.64 kcal/mol. All plant extracts exhibited a 50% inhibitory concentration (IC50) of < 30 μg/mL against MDA-MB 231 cells. Flow cytometry analysis of treated MDA-MB 231 cells showed that the dichloromethane extracts from S. petersiana, Z. mucronate, and ethyl acetate extracts from R. caffra and S. molle induced higher levels of apoptosis than cisplatin. It was concluded that norajmaline could emerge as a potential antimycobacterial lead compound. Validation of the antimycobacterial activity of norajmaline will need to be performed in vitro and in vivo before chemical modifications to enhance potency and efficacy are done. S. petersiana, Z. mucronate, R.caffra and S. molle possess strong potential as key contributors in developing new and effective treatments for triple-negative breast cancer in light of the urgent requirement for innovative therapeutic solutions.

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Section: Discussionmentioning

confidence: 99%

In-silico and in-vitro assessments of some fabaceae, rhamnaceae, apocynaceae, and anacardiaceae species against Mycobacterium tuberculosis H37Rv and triple-negative breast cancer cells

Nyambo

Adu-Amankwaah

Tapfuma

et al. 2023

BMC Complement Med Ther

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“…Qualitative Preliminary Phytochemical Analysis of Leaf Shigella sp, and Salmonella sp). Compound 1 exhibited moderate antimicrobial activity against Salmonella sp with 25 mg/ml MIC values compared to compound 2(Bitombo et al 2021).…”

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confidence: 99%

In vitro Callus Induction of Rauvolfia tetraphylla (L.) an Endangered Medicinal Plant and Evaluation of Biological Activities

Swaminathan¹,

Nallasamy²,

Krishnamoorthy³

et al. 2022

EEC

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Rauvolfiatetraphylla (L.) is a critically endangered woody shrub that is one among the most significantApocynaceae family species cultivated for its therapeutic benefits. The goal of this work is to standardizethe micropropagation methodology for Rauvolfiatetraphylla (L.). Explants wereused to make the callus(Leaf, Internode, and Anther). âWhen leaf explants were grown on Murashige and Skoog (MS) mediasupplemented with Indole-3-Acetic Acid (IAA) (0.5mg/ml), a maximum of 94.2 percent of callus initiationwas observedâ.âOn MS media with 2,4-dichloro phenoxy acetic acid (2,4-D) + Indole-3-Acetic Acid (IAA)(0.5+0.5mg/ml), internodal explants demonstrated a maximum of callus initiation of77.9%â.âOn MS mediawith 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (2,4-D) (0.5 mg/ml) alone,the greatestcallus initiation (89.8%) was found in anther explantsâ. The qualitative preliminary phytochemical analysiswas carried out from leaf and fruit extracts. Extracts were obtained from various organic solvents by thesequential method. Alkaloids, carbohydrates, fixed oils and fats, phenolic compounds, and tannins wereidentified from various crude extracts such as Hexane, Chloroform, Ethyl acetate, and Methanol. Inantibacterial activity, the maximum inhibition was observed in methanol extract of leaf and fruit againstEscherichia coli (100 ïg/ml).

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Microbial allies: exploring fungal endophytes for biosynthesis of terpenoid indole alkaloids

Khalkho,

Beck,

Priyanka

et al. 2024

Arch Microbiol

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Antimicrobial and cytotoxic activities of indole alkaloids and other constituents from the stem barks of Rauvolfia caffra Sond (Apocynaceae)

Cited by 10 publications

References 41 publications

In-silico and in-vitro assessments of some fabaceae, rhamnaceae, apocynaceae, and anacardiaceae species against Mycobacterium tuberculosis H37Rv and triple-negative breast cancer cells

In-silico and in-vitro assessments of some fabaceae, rhamnaceae, apocynaceae, and anacardiaceae species against Mycobacterium tuberculosis H37Rv and triple-negative breast cancer cells

In vitro Callus Induction of Rauvolfia tetraphylla (L.) an Endangered Medicinal Plant and Evaluation of Biological Activities

Microbial allies: exploring fungal endophytes for biosynthesis of terpenoid indole alkaloids

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