Rauvolfiatetraphylla (L.) is a critically endangered woody shrub that is one among the most significantApocynaceae family species cultivated for its therapeutic benefits. The goal of this work is to standardizethe micropropagation methodology for Rauvolfiatetraphylla (L.). Explants wereused to make the callus(Leaf, Internode, and Anther). âWhen leaf explants were grown on Murashige and Skoog (MS) mediasupplemented with Indole-3-Acetic Acid (IAA) (0.5mg/ml), a maximum of 94.2 percent of callus initiationwas observedâ.âOn MS media with 2,4-dichloro phenoxy acetic acid (2,4-D) + Indole-3-Acetic Acid (IAA)(0.5+0.5mg/ml), internodal explants demonstrated a maximum of callus initiation of77.9%â.âOn MS mediawith 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (2,4-D) (0.5 mg/ml) alone,the greatestcallus initiation (89.8%) was found in anther explantsâ. The qualitative preliminary phytochemical analysiswas carried out from leaf and fruit extracts. Extracts were obtained from various organic solvents by thesequential method. Alkaloids, carbohydrates, fixed oils and fats, phenolic compounds, and tannins wereidentified from various crude extracts such as Hexane, Chloroform, Ethyl acetate, and Methanol. Inantibacterial activity, the maximum inhibition was observed in methanol extract of leaf and fruit againstEscherichia coli (100 ïg/ml).
Rauvolfiatetraphylla L. is a significantly endangered woody shrub that belongs to the familyApocynaceaeand it has enormous medicinal properties. Although the plant Rauvolfiatetraphylla is used to treat cancer inthe traditional medicine of some regions, its cytotoxic activity has not been subjected to rigorous investigation.Extracts were obtained from various organic solvents such as Hexane, Chloroform, Ethylacetate andMethanol by the sequential method. The antioxidant activity was accomplished on various crude extractsby 2-diphenyl-1-picrylhydrazyl (DPPH) assay of R. tetraphylla and showed maximum radical scavengingactivity in methanol extract of fruit (IC50 = 60.37 μg/ml) followed by methanol extract of theleaf (IC50 =62.61μg/ml). Screening of in vitrocytotoxicity was performed on all the crude extracts by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay on Breast Cancer cell line (MDAMB231).Among the four-leaf extracts, maximum cytotoxicity activity was obtained by methanol 73.20 μg/mL andthe IC50 value 64.29 μg/ml. Fruit extracts showed maximum cytotoxicity in 65.30 μg/mL methanol andIC50 values were 74.84μg/ml.
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