Antimicrobial and antioxidant potential of different solvent extracts of the medicinal plant Geum urbanum L.

Abstract

Many Geum species are known to be rich in biologically active compounds and therefore could be a source of new natural products with pharmacological potential. The medicinal plant Geum urbanum L. is widespread in Bulgaria and has been used in folk medicine. In the present study, the methanol extracts of the roots and aerial parts of G. urbanum and their fractions (petroleum ether, ethyl acetate and n-butanol) were investigated for antibacterial and radical scavenging activity. The ethyl acetate and n-butanol fractions inhibited the growth of Gram-positive pathogenic and opportunistic bacteria from the genus Staphylococcus (MIC EtOAc: 0.078 mg/ml aerial and 0.156 mg/ml roots; MIC n-BuOH: 0.156 mg/ml aerial and 1.25 mg/ml roots) and the species Bacillus cereus stronger than the other extracts and fractions tested (MIC EtOAc: 0.078 mg/ml aerial and 0.156 mg/ml roots; MIC n-BuOH: 0.156 mg/ml aerial and 0.078 mg/ml roots), and showed corresponding radical scavenging activity (EtOAc: EC50 1.5 µg/ml aerial, 0.8 µg/ml roots; n-BuOH: 4.5 µg/ml aerial; 3.7 µg/ml roots). Additionally, their total phenolic content was quantified (% of dry EtOAc fractions of roots 61%, of arial parts 32%; of dry n-BuOH fractions of roots 16%, of arial parts 13%). Seven compounds were isolated and identified spectroscopically from the ethyl acetate extract. Two acetylated ellagic acid rhamnosides were found for the first time in the genus Geum and three others, tormentic acid, niga-ichigoside F1, and 3,3′-di-O-methylellagic acid-4-O-β-d-glucopyranoside, were newly detected for the species G. urbanum. Our results reveal that G. urbanum L. is a perspective medicinal plant and deserves further, more detailed studies. Electronic supplementary materialThe online version of this article (10.1186/s13065-017-0343-8) contains supplementary material, which is available to authorized users.

Investigation of Antioxidant and Elastase Inhibitory Activities ofGeum urbanumAerial Parts, Chemical Characterization of Extracts Guided by Chemical and Biological Assays

“…The results are shown in Table 1. As previously described for roots and aerial parts of G. urbanum, 15 the EAF had the best antioxidant activities. n-BF exhibited the best DPPH scavenging activity (halfmaximal inhibitory concentration [IC 50 ] <12.5 µg/mL) followed by EAF and GUM (IC 50 14.7 µg/mL and 21.8 µg/ mL, respectively).…”

“…47 Among the compounds elucidated by dereplication, 6-O-Ecaffeoyl-βd-glucopyranose, succinic acid, and 3-(3,4-dihydroxyphenyl)propyl-glycoside are reported here for the first time in the genus Geum. Eight compounds were previously isolated from G. urbanum roots: tormentic acid, 15 ellagic acid pentoside, 16 ellagic acid, gallic acid, caffeic acid, (+)-catechin, 15,17 malic acid, 18 and dotorioside II. 11 The 3 other compounds were already known in the genus Geum.…”

“…11 The 3 other compounds were already known in the genus Geum. 31,46,48 Two compounds previously isolated in G. urbanum roots, 15,16 the ellagic tannins 4 and 7, could be considered as chemotaxonomic markers of this species.…”

“…Recently, the radical scavenging activity of extracts and fractions obtained from aerial and underground parts of G. urbanum were investigated and the ethyl acetate (EtOAc) fractions from roots and aerial parts were found to be the most active, but only the EtOAc extract of the roots was investigated. 15 Thus, the aim of the present study was to investigate the antioxidant and elastase inhibitory activities of extracts, fractions and isolated compounds obtained from the aerial parts of G. urbanum. A 13 C nuclear magnetic resonance (NMR)-based dereplication methodology combined with a bioguided fractionation and purification procedure was used to identify some of its metabolites.…”

Chemical composition and antibacterial activity of essential oil of Tetraclinis articulata (Vahl) Masters branches of eastern Morocco

“…In order to overcome the problem of resistance of microorganisms to antibiotics, most of the work is currently oriented towards other antimicrobial agents with a very specific mode of action. Thus, researchers and scientists are trying to find effective and accessible alternatives from natural products extracted from medicinal and aromatic plants, which are known to be endowed with antimicrobial properties [3][4][5][6][7][8][9][10][11].…”