Antimicrobial and antioxidant activities of Artemisia herba-alba essential oil cultivated in Tunisian arid zone

Mighri, Hédi; Hajlaoui, Hafedh; Akrout, Ahmed; Najjaa, Hanen; Neffati, Mohamed

doi:10.1016/j.crci.2009.09.008

“…Similar results were found with essential oils of several Artemisia species such as A. absinthum, A. biennis, A. cana, A. dracunculus, A .frigida, A. longifolia, A. herba-alba and A. ludoviciana, which are dominant by non-phenolic components (LopesLutz et al 2008;Mighri et al 2010). The essential oil of A. annua has antioxidant activity and metal chelating activity similar to thymol, a known antioxidant (C´avar et al 2012).…”

Section: Free Radical-scavenging Activity: Dpph Assaysupporting

confidence: 69%

The Effect of Different Harvest Stages on Chemical Composition and Antioxidant Capacity of Essential Oil from Artemisia annua L.

Şenkal

¹

,

Kıralan

²

,

Yaman

³

2014

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Chemical composition of the essential oils obtained by hydro-distillation from Artemisia annua L. (Asteraceae) harvested before flowering (BF), 50% of flowering (50%F), full flowering (FF), and after flowering (AF) stages were determined using GC and GC/MS analysis. The essential oil contents were 0.8%, 0.96%, 1.22% and 1.38% in BF, 50% F, FF and AF, respectively. In total, 20 compounds were identified, with artemisia ketone (28.30%-37.15%), camphor (18.00%-23.30%), and 1,8-cineole (9.00%-10.39%) as main components. The highest amounts of the three main components were recorded in the essential oils of the plants harvested in the FF stage. Also, the free radicals scavenging activity of the essential oils, tested by using DPPH method, were found to be in order of FF>50% F>AF>BF. ÖZETÇiçeklenme öncesi (ÇÖ), % 50 çiçeklenme (% 50 Ç), tam çiçeklenme (TÇ) ve çiçeklenme sonrası (ÇS) dönemlerinde hasat edilen Artemisia annua L. (Asteraceae)'dan su distilasyonu yöntemiyle elde edilen uçucu yağların kimyasal kompozisyonu GC ve GC/MS kullanılarak belirlenmiştir. Uçucu yağ içerikleri ÇÖ, % 50 Ç, TÇ ve ÇS dönemlerinde sırasıyla % 0.8, % 0.96, % 1.22 ve % 1.38 bulunmuştur. Toplamda 20 bileşenin tespiti yapılmış olup, artemisia ketone (% 28.30-% 37.15), camphor (% 18.00-% 23.30) ve 1,8-cineole (% 9.00-% 10.39) ana bileşenler olarak belirlenmiştir. Üç ana bileşene ait en yüksek değerlere tam çiçeklenme döneminde hasat edilen bitkilerin uçucu yağlarında kaydedilmiştir. Ayrıca, DPPH yöntemiyle test edilen uçucu yağların serbest radikal yakalama aktivitesi TÇ˃% 50 Ç˃ÇS˃ÇÖ sırasında bulunmuştur.

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“…It is known that essential oils obtained from plants belonging to the Artemisia genus are markedly rich in non-phenolic constituents (Lopes-Lutz et al 2008). The essential oils having non-phenolic compounds showed the weak antioxidant activity (Juteau et al 2002;Mighri et al 2010). The essential oil of A. annua aerial parts, consisting of camphor (44%), germacrene D (16%), trans-pinocarveol (11%), β-selinene (9%), β-caryophyllene (9%) and artemisia ketone (3%), was observed the weak antioxidant activity (Juteau et al 2002).…”

Section: Free Radical-scavenging Activity: Dpph Assaymentioning

confidence: 99%

The effect of different harvest stages on chemical composition and antioxidant capacity of essential oil from Artemisia annua L.

ŞENKAL¹,

KIRALAN²,

YAMAN³

et al. 2015

Tarım Bilimleri Dergisi

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Chemical composition of the essential oils obtained by hydro-distillation from Artemisia annua L. (Asteraceae) harvested before flowering (BF), 50% of flowering (50%F), full flowering (FF), and after flowering (AF) stages were determined using GC and GC/MS analysis. The essential oil contents were 0.8%, 0.96%, 1.22% and 1.38% in BF, 50% F, FF and AF, respectively. In total, 20 compounds were identified, with artemisia ketone (28.30%-37.15%), camphor (18.00%-23.30%), and 1,8-cineole (9.00%-10.39%) as main components. The highest amounts of the three main components were recorded in the essential oils of the plants harvested in the FF stage. Also, the free radicals scavenging activity of the essential oils, tested by using DPPH method, were found to be in order of FF>50% F>AF>BF. ÖZETÇiçeklenme öncesi (ÇÖ), % 50 çiçeklenme (% 50 Ç), tam çiçeklenme (TÇ) ve çiçeklenme sonrası (ÇS) dönemlerinde hasat edilen Artemisia annua L. (Asteraceae)'dan su distilasyonu yöntemiyle elde edilen uçucu yağların kimyasal kompozisyonu GC ve GC/MS kullanılarak belirlenmiştir. Uçucu yağ içerikleri ÇÖ, % 50 Ç, TÇ ve ÇS dönemlerinde sırasıyla % 0.8, % 0.96, % 1.22 ve % 1.38 bulunmuştur. Toplamda 20 bileşenin tespiti yapılmış olup, artemisia ketone (% 28.30-% 37.15), camphor (% 18.00-% 23.30) ve 1,8-cineole (% 9.00-% 10.39) ana bileşenler olarak belirlenmiştir. Üç ana bileşene ait en yüksek değerlere tam çiçeklenme döneminde hasat edilen bitkilerin uçucu yağlarında kaydedilmiştir. Ayrıca, DPPH yöntemiyle test edilen uçucu yağların serbest radikal yakalama aktivitesi TÇ˃% 50 Ç˃ÇS˃ÇÖ sırasında bulunmuştur.

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“…This spectrophotometric assay uses the stable radical DPPH (2,2-Diphenyl-1-picrylhydrazyl) as a reagent [29,30] . Radical scavenging activity of extracts was measured by slightly modified method of Mighri et al and Braca et al as described below [31,32] . Different concentrations of each extract were prepared in ethanol: 0.1 -1 mg/mL.…”

Section: Antioxidant Activity Evaluation Dpph (22-diphenyl-1-picrylhmentioning

confidence: 99%

Study on the antioxidant activities of root extracts of Zizyphus lotus from the western region of Algeria

Ghalem¹,

Merghache²,

Belarbi³

2014

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Objective: To find a new natural source of antioxidant, the phenolic contents and antioxidant activities of some secondary metabolites extracted from roots of Ziziphus lotus were evaluated. Methods: The total polyphenol contents were determined spectrophotometrically. Pyrrocatechol, catechin and cyanidin equivalents were used for these parameters. The antioxidant activities of the extracts were determined by several in vitro systems of assays, namely DPPH radical scavenging activity method, b-Carotene Bleaching assay (BCB), Ferric Reducing Antioxidant Power assay (FRAP) and Total Antioxidant capacity test (TAC). Results: The quantitative estimation showed that the roots of Z. lotus were rich in polyphenols (20.09 mg PE/g DW) and proanthocyannidins (1.56%) and they contained a small amount of flavonoids (0.02 mg CE/g DW). The most fractions exhibited high antioxidant activities, and some even showed higher potency than the standard synthetic antioxidants in some instances. In DPPH assay, all extracts had shown significant inhibition (58.535 -94.730% at 1 mg/mL). In addition, the IC 50 values ranged from 0.211 to 0.816 mg/mL, compared to 0.110, 0.214, 0.214 and 0.413 mg/mL for gallic acid, tannic acid, butylated-hydroxyanisol and ascorbic acid respectively. In (BCB) assay, the extracts showed strong inhibition (55.55 -100.00% at 1 mg/mL) and the IC 50 values ranging from 0.123 to 0.850 mg/mL compared to 0.433 mg/mL for gallic acid. A dose dependant curve was obtained for all extracts in the FRAP assay. However, the antioxidant potencies of ascorbic acid and extracts were comparable at low concentrations. The majority of extracts showed the highest value of antioxidant activity, based on TAC test (0.073 -0.398 mg ascorbic acid / mg extract). Conclusion: The results indicate that Z. lotus roots could be an important sources of natural antioxidants.

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Antimicrobial and antioxidant activities of Artemisia herba-alba essential oil cultivated in Tunisian arid zone

Cited by 117 publications

References 18 publications

The Effect of Different Harvest Stages on Chemical Composition and Antioxidant Capacity of Essential Oil from Artemisia annua L.

The Effect of Different Harvest Stages on Chemical Composition and Antioxidant Capacity of Essential Oil from Artemisia annua L.

The effect of different harvest stages on chemical composition and antioxidant capacity of essential oil from Artemisia annua L.

Study on the antioxidant activities of root extracts of Zizyphus lotus from the western region of Algeria

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