Antimicrobial and antifungal effects of tissue conditioners containing a photocatalyst

Uchimaru, Masayuki; Sakai, Takako; Moroi, Ryoji; Shiota, Susumu; Shibata, Yukie; Deguchi, Masahiro; Sakai, Hidetaka; Yamashita, Yoshihisa; Terada, Yoshihiro

doi:10.4012/dmj.2010-203

Cited by 17 publications

(26 citation statements)

References 28 publications

(25 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Upon UVA irradiation, methylene blue, an organic compound, is effectively removed from a TiHA-containing denture [27]. Several planktonic bacteria from TiHA-containing tissue-conditioning materials are also killed upon UVA irradiation [28]. However, denture plaque mostly consists of bacterial biofilms composed of multilayers of a wide variety of oral bacteria, not of planktonic bacteria.…”

Section: Introductionmentioning

confidence: 99%

Antimicrobial Effect of Titanium Hydroxyapatite in Denture Base Resin

et al. 2018

View full text Add to dashboard Cite

In the current study, we investigated the antimicrobial effect of titanium hydroxyapatite (TiHA), a photo-oxidizing organic material, in denture base resin on single-species biofilms formed by laboratory bacteria and on multispecies biofilms formed by bacteria from the human saliva. Although TiHA reportedly restricts the growth of planktonic bacteria upon ultraviolet A (UVA) irradiation, the antimicrobial effect of TiHA on bacterial biofilms remains to be elucidated. Resin specimens were prepared by adding TiHA to polymethyl methacrylate-based, denture base resin. The specimens were incubated with biofilm-forming Streptococcus sanguinis, Actinomyces naeslundii, Staphylococcus aureus, Escherichia coli, or bacteria from the human saliva obtained from volunteers. After UVA irradiation, the colony-forming units (CFUs) from the biofilms formed on the specimens were determined. CFU numbers for S. sanguinis, A. naeslundii, and S. aureus that formed biofilms on TiHA-containing specimens were significantly lower than those formed on specimens without TiHA. TiHA did not reduce the CFUs of biofilm-forming E. coli. In all cases, CFU numbers in the biofilms formed on TiHA-containing specimens by the salivary bacteria were significantly reduced. In addition, neither a 56 h UVA irradiation nor a 28 d soaking in water diminished the antibacterial effect of TiHA. TiHA in denture base resin exerts an antimicrobial effect on single-species bacterial biofilms and biofilms formed by a wide variety of bacteria from human saliva.

show abstract

Section: Introductionmentioning

confidence: 99%

Antimicrobial Effect of Titanium Hydroxyapatite in Denture Base Resin

et al. 2018

View full text Add to dashboard Cite

show abstract

“…In microbiological assays, nystatin restricted the emergence of C. albicans when it was used with a tissue conditioner. 24,30 Some researchers added nystatin to the material as a fungicidal factor. Douglas and Walker 21 showed that the tissue conditioners repressed colonization when used with nystatin and validated their findings in an in vivo study.…”

Section: Although the Etiology Of Denture Stomatitis Is Multifactoriamentioning

confidence: 99%

“…8 Martínez-Beneyto et al 35 showed that the most 9 recommended fungicidal agent was miconazole (prescribed by 59.3% of the survey's respondents), followed by nystatin (57.7%) for topical use. Uchimaru et al 24 reported that TiO 2 , which is a stable photocatalyst, combined with a tissue conditioner exhibited antimicrobial activity against Escherichia coli, S. mutans, and S. aureus, and antifungal activity against C. albicans. TiO 2 exhibits strong oxidizing power under UV radiation (from sunlight or an illuminated light source) 36 but no photocatalystic effects at nonradiation, 37,38 which means soft liners with TiO 2 may have no antifungal effect in the oral cavity environment.…”

mentioning

confidence: 99%

Comparison of the Effect of Nystatin and Titanium Dioxide Addition on Candida Colonization in Soft Lining Material

Bayındır¹,

Aktaş²,

Baştopçu³

2017

Atatürk Üniversitesi Diş Hekimliği Fakültesi Dergisi

View full text Add to dashboard Cite

Material and methods. Disks of Ufi Gel P and Mollosil cold-curing, soft relining materials with a diameter of 5 mm and thickness of 2 mm were prepared with nystatin and TiO 2 powders in a concentration of 5%, 10%, and 15% (w/v). For the control group, only discs not containing nystatin and TiO 2 were prepared, which consisted only of soft lining material. C. albicans was inoculated on Sabouraud dextrose agar (SDA)-coated plates. The disks were placed on these plates and incubated aerobically at 37° C.The Ufi Gel P and Mollosil disks, including 10% w/v nystatin, (re-prepared for the second step of the study) were immersed in water for separate intervals (1, 7, 14, and 16 days) to determine the fungicidal action over time. The disks were then removed and placed on SDA plates inoculated with C. albicans. In each group, one control disk without nystatin or TiO 2 was included. Results.A univariate analysis of variance test revealed that the activity of C. albicans was not significantly inhibited on the control disks (p < 0.001). The addition of nystatin in powder form to the soft liners prevented the colonization of C. albicans in vitro. The immersion time of the disks plunged in water appeared to affect the degree of inhibition. However, the addition of TiO 2 did not prevent colonization by C. albicans of the soft liners.Conclusions. This study showed that the addition of nystatin in powder form to soft liners prevented the colonization of C. albicans in vitro but that the addition of the same amount of TiO 2 powder did not.

show abstract

“…Metals, such as copper and silver, have been suggested, but it has been indicated that some metals can have harmful effects (e.g., metal allergy). [4][5][6][7] Other studies have looked at developing nonmetal antimicrobial tissue conditioners, which use the antimicrobial protein human lactoferrin (hLF) commonly found in saliva, 8 photocatalysts, 9,10 or antimicrobial agents, [11][12][13] and even Melaleuca alternifolia. 14 Juncus effuses has antimicrobial activity against many microorganisms.…”

mentioning

confidence: 99%