Antimicrobial and antibiofilm efficacy and mechanism of essential oil from Citrus Changshan-huyou Y. B. chang against Listeria monocytogenes

Guo, Jiajing; Gao, Zhipeng; Li, Gaoyang; Fu, Fuhua; Liang, Zengenni; Zhu, Huaqun; Shan, Yang

doi:10.1016/j.foodcont.2019.06.014

Cited by 59 publications

(32 citation statements)

References 39 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…To form mature biofilm of L. monocytogenes, the bacterial suspension was cultured overnight at 37 • C, then 2000 µl bacteria suspension were added to each well (with embedded coverslips) in 24-well tissue culture plates and incubated at 37 • C for 24 h, then the fresh broth was added to each well after remove the old broth, and incubated for 24 h, and then the broth was changed again and incubated for another 24 h. After incubated for 72 h in total, 2000 µl fresh broth containing linalool (1 × MIC concentration) was added to each well, then the plates were incubated for a series of time intervals (8, 16, and 24 h) at 37 • C, the broth without linalool added was used as positive control (Guo et al, 2019). After incubation, the old broth were removed and the samples were washed with PBS three times.…”

Section: Growth Experimentsmentioning

confidence: 99%

Anti-listeria Activities of Linalool and Its Mechanism Revealed by Comparative Transcriptome Analysis

et al. 2019

Self Cite

View full text Add to dashboard Cite

Listeria monocytogenes, which causes serious foodborne infections and public health problems worldwide, is one of the most important foodborne pathogens. Linalool has been identified as an antimicrobial agent against some microorganism, but its mechanism of action is currently unclear. Here, we investigated the efficacy of linalool against L. monocytogenes while planktonic and as a biofilm and explored potential mechanisms of action. Linalool exhibited strong anti-listeria activity in the planktonic stage. Scanning electron microscopy (SEM) and transmission electron microscopy (TEM) observations revealed seven stages were classified of cells at microscopic level. Mesosome-like structures were observed for the first time in L. monocytogenes after linalool treatment. Linalool also showed significant anti-biofilm activity through both dispersal and killing of cells in the biofilm based on confocal scanning laser microscopy (CLSM) and SEM imaging, crystal violet staining, XTT and COMSTAT assays. Moreover, comparative transcriptome analysis demonstrated many potential mechanisms of action for linalool and some important pathways were screened out through the analysis of GO enrichment and KEGG. Our study provides evidence that linalool exhibits a strong antimicrobial activity against both the planktonic and biofilm forms of L. monocytogenes and gives insight into its mechanism of action.

show abstract

Section: Growth Experimentsmentioning

confidence: 99%

Anti-listeria Activities of Linalool and Its Mechanism Revealed by Comparative Transcriptome Analysis

et al. 2019

Self Cite

View full text Add to dashboard Cite

show abstract

“…Changes in the expression levels of genes were confirmed by qRT-PCR, and it was found that the genes related to oxidative stress were up-regulated, and the genes related to cell wall synthesis were down-regulated after pterostilbene treatment. The essential oil from Huyou plant could effectively destroy the cell membrane and biofilm of Listeria monocytogenes , and RNA sequencing results showed that differentially expressed genes were enriched in pathways such as protein export, bacterial secretion systems, ABC transporter, and quorum sensing (Guo et al, 2019). A transcriptomic analysis revealed the upregulation of related stress resistance genes in essential oil-adapted E. coli cells (treated with antibacterial agents at one-half MIC), and these cells have shown enhanced resistance against subsequent lethal essential oil (Yuan et al, 2018).…”

Section: Discussionmentioning

confidence: 99%

Structure-Dependent Inhibition of Stenotrophomonas maltophilia by Polyphenol and Its Impact on Cell Membrane

et al. 2019

View full text Add to dashboard Cite

As natural occurring antimicrobial substances, phenolic compounds have been used to inhibit various bacteria. Stenotrophomonas maltophilia 4–1, a strain isolated from food, exhibited spoilage potential in vitro with proteolysis and lipolysis at 25°C. The present study evaluated the antibacterial properties of 13 polyphenols on S. maltophilia 4–1, and selected 6 compounds (ferulic acid, p-coumaric acid, caffeic acid, chlorogenic acid, (−)-epigallocatechin, and phloretin) for binary combination treatments. The results revealed that antibacterial activities of polyphenols were structure-dependent, and cinnamic acid showed strong inhibitory effects, with a minimum inhibitory concentration (MIC) of 0.125 mg/mL. Importantly, we did not observe any obvious synergistic effects across all binary combinations. The antibacterial mechanism of cinnamic acid was related to membrane damage, caused by the loss of cell membrane integrity and alteration of cell morphology. These findings suggest that cinnamic acid is a promising candidate for the control of spoilage bacteria in food.

show abstract

“…L. monocytogenes is one of the most important foodborne pathogens (Guo et al, 2019) and can cause an important public health problem with high mortality and morbidity rates (Lee et al, 2018;Stratakos, et al, 2020). L. monocytogenes infection entitled listeriosis can lead to disease during vulnerable stages of life such as newborn, pregnant woman, elderly and persons with immunocompromising conditions individuals (Gomez et al, 2014).…”

Section: Introductionmentioning

confidence: 99%

“…L. monocytogenes can be found in various food groups such as milk and dairy products, meat and meat products, seafood, vegetables and ready-to-eat foods (Lee et al, 2018). L. monocytogenes grows between 0.4 and 50 °C (Zhu et al, 2017;Guo et al, 2019). Therefore, the development of this pathogen in refrigerator conditions makes food preservation difficulties.…”

Section: Introductionmentioning

confidence: 99%

Antibacterial Activity of Different Essential Oils on Listeria Monocytogenes Strains Isolated From Ready-to-Eat Foods

Aktop

Canberi

Şentürk

et al. 2020

Gıda

View full text Add to dashboard Cite

Listeria monocytogenes is one of the food-borne pathogens that cause major health problems worldwide. Application of essential oils (EOs) is used to control this pathogen and reduce microbial levels. The aim of the present study was to investigate the antibacterial activity of 15 different EOs obtained from plants on L. monocytogenes strains from ready-to-eat foods. In this study, thyme oil (mean zone 24.850±3.714 mm) showed the highest antibacterial activity against L. monocytogenes. Clove oil (mean zone 12.383±2.215 mm) and sage oil (mean zone 11.117±3.170 mm) were also determined high antibacterial activity. Ginger oil and garlic oil did not have any antibacterial activity against L. monocytogenes strains. This study shows that using of EOs against food-borne pathogens in food systems could be useful.

show abstract

Antimicrobial and antibiofilm efficacy and mechanism of essential oil from Citrus Changshan-huyou Y. B. chang against Listeria monocytogenes

Cited by 59 publications

References 39 publications

Anti-listeria Activities of Linalool and Its Mechanism Revealed by Comparative Transcriptome Analysis

Anti-listeria Activities of Linalool and Its Mechanism Revealed by Comparative Transcriptome Analysis

Structure-Dependent Inhibition of Stenotrophomonas maltophilia by Polyphenol and Its Impact on Cell Membrane

Antibacterial Activity of Different Essential Oils on Listeria Monocytogenes Strains Isolated From Ready-to-Eat Foods

Contact Info

Product

Resources

About