Antimicrobial and antibiofilm activity of<i>Baccharis psiadioides</i>essential oil against antibiotic-resistant<i>Enterococcus faecalis</i>strains

Negreiros, Mateus de Oliveira; Pawlowski, Ângela; Zini, Cláudia Alcaraz; Soares, Geraldo Luiz Gonçalves; Motta, Amanda de Souza da; Frazzon, Ana Paula Guedes

doi:10.1080/13880209.2016.1223700

Cited by 36 publications

(22 citation statements)

References 55 publications

(58 reference statements)

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“…Broth microdilution assay was carried out in a sterile U-bottomed 96-well polystyrene microtitre plate, as described by NEGREIROS et al (2016). In each well of a polystyrene microtitre plate, 100 µL of Müeller-Hinton broth (MHB, HiMedia Laboratories) was dispensed, followed by 100 µL of the EO dl ES into the first well and serial dilutions -to achieve the final oil concentrations of 50 to 0.09% -of the EO dl ES.…”

Section: Determination Of Minimum Inhibitory Concentration Of Eucalypmentioning

confidence: 99%

“…In recent years, compounds with biological properties extracted from fresh leaves have received attention as an alternative to control microorganisms (NEGREIROS et al, 2016, SELIM et al, 2014. However, according to data, there are few reports antimicrobial activity of essential oils extracted from dried leaves (RADAELLI et al, 2016;SEMENIUC et al, 2017).…”

Section: Introductionmentioning

confidence: 99%

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Antimicrobial and antibiofilm activity of the essential oil from dried leaves of Eucalyptus staigeriana

et al. 2019

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In recent years, compounds with biological properties produced by plants have received attention as an alternative to control microorganisms. Essential oils extracted from green leaves of Eucalyptus sp. have been demonstrated to have antimicrobial activities, but so far there are no reports of antimicrobial activity of essential oils extracted from dried leaves of Eucalyptus staigeriana. So, the objectives of this study were to determine the chemical composition of the essential oils obtained from dried leaves of E. staigeriana (EOdlES) and to evaluate in vitro antimicrobial and antibiofilm activities of EOdlES against gram-positive and gram-negative, resistance and multiresistant Enterococcus faecalis isolated from food and clinical samples. The characterization of EOdlES was performed by gas chromatography-mass spectrometry (GC/MS). For this study, 26 bacterial strains were used, which included 11 reference strains and 15 antibiotic resistant and multiresistant E. faecalis strains. Antimicrobial activities of EOdlES against gram-positive and gram-negative were determined using the disc diffusion method. The minimum inhibitory concentration (MIC) value was evaluated by a microbroth dilution technique. The antibiofilm effects were assessed by microtiter plate method. As a result, 21 compounds were identified, being oxygenated monoterpenes (69.58%) the major chemical family. EOdlES showed only antimicrobial activity against gram-positive strains. E. faecalis resistant and multiresistant strains show the lowest MIC (3.12 to 6.25%), when compared with reference E. faecalis strain. EOdlES has the ability to inhibit the biofilm formation, but little or none ability to inhibit the preformed biofilm. This study demonstrates that EOdlES is a promising alternative to control important foodborne and clinic gram-positive resistant bacteria.

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Section: Determination Of Minimum Inhibitory Concentration Of Eucalypmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Antimicrobial and antibiofilm activity of the essential oil from dried leaves of Eucalyptus staigeriana

et al. 2019

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“…The microdilution method in 96‐well microtiter plates were used to determine Minimum inhibitory concentrations (MIC), as defined by Negreiros et al . with some modifications.…”

Section: Methodsmentioning

confidence: 99%

Evaluation of Phytochemical Compositions and Biological Properties of Achillea gypsicola at Different Phenological Stages

Açıkgöz

2019

Chemistry & Biodiversity

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Phytochemicals, which are commonly found at different levels in many medicinal plants, are natural strong antioxidants used in traditional medicine. In this research, determination of differences of phytochemical compositions and biological properties were aimed as periodically (pre-, full and post flowering) and daily (6 am, 1 pm and 8 pm) in Achillea gypsicola HUB.-MOR. The volatile oils belonging to A. gypsicola were obtained by hydrodistillation and analyzed by gas chromatography-flame ionization detection (GC-FID) and gas chromatography-mass spectrometry (GC/MS). The antimicrobial activities of the volatile oils were determined with disc diffusion method. The microdilution method was used to determine minimum inhibitory concentration (MIC). Total phenolic and flavonoid contents were determined by spectrophotometric methods and antioxidant capacities were evaluated by 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radical, reducing power (RP) and metal chelating activity (MCA) assay. In addition, the phenolic acid and flavonoid compositions were evaluated by reversed phase-high-performance liquid chromatography (RP-HPLC). This study presented a comprehensive report for the first time on evaluation of the phytochemical composition and the biological properties of A. gypsicola at different phenological stages. Thirty-two compounds, containing the major component as camphor, 1,8-cineole and borneol, were detected. Designated harvest time for the highest yield of volatile oils was found to be at full flowering stage-1 pm. It has been observed that the volatile oil composition changes periodically and even daily. Also, in this research, menthol and menthone were found as the composition of volatile oil in Achillea species for the first time. Full flowering stage was found as the richest period in terms of phenolic acid and flavonoid compositions of A. gypsicola for the first time. The species examined in this research showed a high antioxidant and antimicrobial activity in comparison to other studies with Achillea species. The volatile oils exhibited high performances with range of inhibition zones (8.3-42.3 mm) and minimum inhibitory concentration values (2.25-144 μg/ml). Besides, a high correlation between antioxidant activity and phenolic content of A. gypsicola was found. These results suggest that A. gypsicola can be used as a safe source in the cosmetic, food and pharmaceutical industries.

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“…The Lm55 strain was cultivated in tryptone soy broth (TSB; HiMedia, Mumbai, Maharashtra, India) at 37°C under agitation. The MIC/2 of EOBp (Negreiros et al 2016) was added in the exponential growth phase, when the microorganism had reached an optical density (OD 600 nm ) between 0.3 and 0.4, measured with an ultraviolet/visible spectrophotometer (Ultrospec 3100 Pro; Amersham Biosciences, Little Chalfont, UK). After 20 min, growth was interrupted and cells were washed with 300 μL 1X TE buffer (10 mM Tris-HCl pH 8.0, 1 mM EDTA pH 8.0; reagents from Sigma-Aldrich) and resuspended in 100 μL 1X TE buffer.…”

Section: Experimental Design Rna Sequencing and Statistical Analysesmentioning

confidence: 99%

“…Müller (= Heterothalamus psiadioides Less.) (Giuliano and Freire 2011), has important anti-inflammatory properties (Fabri et al 2011) and the ability to inhibit the growth of antibiotic resistant microorganisms, also reducing biofilm formation in abiotic surfaces (Negreiros et al 2016). Natural compounds present in the essential oil of B. psiadioides (EOBp) are classified as terpenes, and can be divided into two fractions: (1) monoterpenes with a significant percentage composed of β-pinene; and (2) sesquiterpenes with Ar-curcumene as the major component.…”

Section: Introductionmentioning

confidence: 99%

Comparative transcriptomic analysis of Listeria monocytogenes reveals upregulation of stress genes and downregulation of virulence genes in response to essential oil extracted from Baccharis psiadioides

et al. 2017

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Listeria monocytogenes is a pathogenic microorganism in humans and is frequently transmitted by food. Methods to control the presence of Listeria in foods are necessary. In the present study, transcriptomics of L. monocytogenes grown in the presence of essential oil extracted from Baccharis psiadioides were studied by RNA sequencing and reverse transcription quantitative polymerase chain reaction (RT-qPCR) experiments. The results obtained indicate that essential oil of B. psiadioides has potential bacteriostatic activity at the concentration tested, affecting Listeria cells functioning and development. Responses of the microorganism included upregulation of stress genes and downregulation of virulence genes, such as actA, hly and prfA, indicating a decrease in virulence and in the capacity of the microorganism to cause infection. Thus, the results presented here allow us to conclude that B. psiadioides essential oil may be an alternative means of controlling microorganisms proliferating in foods.

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Antimicrobial and antibiofilm activity ofBaccharis psiadioidesessential oil against antibiotic-resistantEnterococcus faecalisstrains

Cited by 36 publications

References 55 publications

Antimicrobial and antibiofilm activity of the essential oil from dried leaves of Eucalyptus staigeriana

Antimicrobial and antibiofilm activity of the essential oil from dried leaves of Eucalyptus staigeriana

Evaluation of Phytochemical Compositions and Biological Properties of Achillea gypsicola at Different Phenological Stages

Comparative transcriptomic analysis of Listeria monocytogenes reveals upregulation of stress genes and downregulation of virulence genes in response to essential oil extracted from Baccharis psiadioides

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