Antimicrobial and antiadhesive properties of a biosurfactant isolated fromLactobacillus paracaseissp.paracaseiA20

Abstract

Aims: The aim of this study was to determine the antimicrobial and antiadhesive properties of a biosurfactant isolated from Lactobacillus paracasei ssp. paracasei A20 against several micro-organisms, including Gram-positive and Gram-negative bacteria, yeasts and filamentous fungi. Methods and Results: Antimicrobial and antiadhesive activities were determined using the microdilution method in 96-well culture plates. The biosurfactant showed antimicrobial activity against all the micro-organisms assayed, and for twelve of the eighteen micro-organisms (including the pathogenic Candida albicans, Escherichia coli, Staphylococcus aureus, Staphylococcus epidermidis and Streptococcus agalactiae), the minimum inhibitory concentration (MIC) and the minimum bactericidal concentration (MBC) were achieved for biosurfactant concentrations between 25 and 50 mg ml )1 . Furthermore, the biosurfactant showed antiadhesive activity against most of the micro-organisms evaluated.Conclusions: As far as we know, this is the first compilation of data on antimicrobial and antiadhesive activities of biosurfactants obtained from lactobacilli against such a broad group of micro-organisms. Although the antiadhesive activity of biosurfactants isolated from lactic acid bacteria has been widely reported, their antimicrobial activity is quite unusual and has been described only in a few strains. Significance and Impact of the Study: The results obtained in this study regarding the antimicrobial and antiadhesive properties of this biosurfactant opens future prospects for its use against micro-organisms responsible for diseases and infections in the urinary, vaginal and gastrointestinal tracts, as well as in the skin, making it a suitable alternative to conventional antibiotics.

Antibiofilm Activity of Biosurfactant Producing Coral Associated Bacteria Isolated from Gulf of Mannar

“…A minimal reduction of 6-8 mN m 21 was observed in CFCS of CAB isolates with that of uninoculated ZMB. The small reduction observed in the surface tension implies that these strains produced low level of extracellular biosurfactants which goes well with previous report [5]. The biosurfactant producers were identified to genus and species level by 16S ribosomal RNA gene sequencing.…”

“…They are also explored for their antitumor, antiviral and anti-mycoplasmic activities [3,4]. In recent years, biosurfactants are considered as potential drug candidates and looked up for their explicit biomedical applications such as antibacterial, antifungal, antiviral and antiadhesive agents against several pathogens [4][5][6][7][8]. Biosurfactants have also been proved as potential immunomodulators and enzyme inhibitors [9].…”

Antimicrobial and Anti-Adhesive Potential of a Biosurfactants Produced by Candida Species

“…The list includes surfactin, produced by B. subtilis, the most powerful of biosurfactant known to date. These compounds have many pharmacological activities: antibacterial, antifungal, antiviral, and antimycoplasma properties; inhibition of the fibrin clot formation and hemolysis; formation of ion channels in lipid bilayer membranes [24]; antitumour activity against Ehrlich's ascites carcinoma cells; and inhibition of the cyclic adenosine 3,5-monophosphate phosphodiesterase [25].…”

“…Gudina et al [24] observed an anti-adhesive activity for the biosurfactant from Lactobacillus paracasei against several pathogenic microorganisms such as S. aureus, S. epidermidis and S. agalactiae. However, this biosurfactant showed low anti-adhesive activity against E. coli, C. albicans and P. aeruginosa, in contrast with the antimicrobial activity exhibited against these strains at the same biosurfactant concentrations.…”

Production and Partial Characterization of Pigments Produced by Kocuria Sp Bri 36: Influence of Heavy Metals

“…Antibacterial activity of the crude pigment (5-0.013 mg/ml) against several microbial strains was determined by the 96-well plate microdilution method [13]. Different clinical isolates used were E. coli, Bacillus, Staphylococcus aureus, Pseudomonas aeruginosa, Shigella and Salmonella paratyphi.…”