Antimicrobial analysis of honey against Staphylococcus aureus isolates from wound, ADMET properties of its bioactive compounds and in-silico evaluation against dihydropteroate synthase

Edet, U. O.; Mbim, E; Ezeani, Esu; Henshaw, Okoroiwu Uchechi; Ibor, Oju R.; Bassey, I. U.; Asanga, Edet Effiong; Antai, Ekpo Eyo; Nwaokorie, Francisca O.; Edet, Bassey Okon; Bebia, Glory P; Tega, Curtis; Mboto, C. I.; Nkang, Ani; Nneoyi-Egbe, Ada Francesca

doi:10.1186/s12906-023-03841-z

Cited by 11 publications

(9 citation statements)

References 45 publications

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“…A study in Ethiopia demonstrated that honey had antibacterial activity against tetracycline-resistant S. aureus and MRSA [93,94] . The antibacterial activity of honey could be due to its composition of various chemicals [1,52,58,[95][96][97] . As such, honey may be a potential therapy for infections caused by resistant strains of bacteria such as MRSA.…”

Section: Discussionmentioning

confidence: 99%

Antibacterial activities of honey against Escherichia coli and Staphylococcus aureus: A potential treatment for bacterial infections and alternative to antibiotics

Mudenda¹,

Hikaambo²,

Chabalenge³

et al. 2023

J Pharmacogn Phytochem

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The global problem of antimicrobial resistance (AMR) has increased interest in natural products, such as honey in the treatment of diseases. This study evaluated the antibacterial activities of honey against Escherichia coli (E. coli) and Staphylococcus aureus (S. aureus). This cross-sectional study was conducted from November 2020 to May 2021 using E. coli and S. aureus as indicator organisms. The antibacterial activities of honey were assessed using the agar well diffusion assay. The sensitivities of E. coli and S. aureus to honey were indicated by zones of inhibition which were measured using a ruler in millimetres. Data analysis was performed using Stata version 16.1. Honey produced a dose-dependent antibacterial activity against E. coli and S. aureus. Our study demonstrated that honey had stronger antibacterial activities against E. coli compared to S. aureus. This study highlights the need for further research on honey to investigate its potential use in treating bacterial infections.

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Section: Discussionmentioning

confidence: 99%

Antibacterial activities of honey against Escherichia coli and Staphylococcus aureus: A potential treatment for bacterial infections and alternative to antibiotics

Mudenda¹,

Hikaambo²,

Chabalenge³

et al. 2023

J Pharmacogn Phytochem

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“…Molecular docking has emerged as the most utilized in-silico or computational approach for studying interactions between proteins and other macromolecules with small lead drug compounds [51] [22] [23]. We docked a total of 13 ligands and trimethoprim against dihydropteroate synthase of E. coli and the docking scores for the various ligands ranged from − 4.0 to -5.3(kcal/mole while trimethoprim gave a docking score of -6.5 kcal/mole.…”

Section: Discussionmentioning

confidence: 99%

“…The stored isolates were rst sub-cultured using freshly prepared nutrient agar and incubated overnight at 37 o C. The isolates were then suspended each in sterilized 5 ml peptone water and the concentration of the isolates adjusted to MacFarland standard which holds approximately 10 7 cells/ml. From each standardized inoculum, 1 ml was plated out using surface plating technique on freshly prepared Mueller-Hinton agar and incubated for 1 h. After incubation, holes were bored aseptically as previously reported [23] and various concentrations of the extracts (100, 50 25 and 12.5%) and trimethoprim (100, 50, 25 and 12.50 mg/l) freshly prepared were dispensed in triplicates per plate and incubated at 37 o C overnight. The resulting zones were measured and interpreted as sensitive, intermediate and resistant as previously reported [34].…”

Section: Microbiological Analysismentioning

confidence: 99%

“…To save time and cost, researchers have turned to several strategies and these include repurposing of existing approved drugs [21]. Yet, other have turned to screening bioactive compounds from medicinal plants [22] [23]. In this study, the aim is to evaluate the antimicrobial activity of T. Tetraptera (Uyayak) pod that is widely used in cooking soups and in folk medicine to manage various ailments using in-vitro and in-silico approaches against E. coli isolates from street vended RTE fruits and salads.…”

Section: Introductionmentioning

confidence: 99%

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Antimicrobial activity of Tetrapleura tetraptera (Uyayak) extracts, ADMET profiling and molecular docking of its bioactive compounds against dihydropteroate synthase of Escherichia coli

Bebia

Edet²,

Eyo

et al. 2023

Preprint

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Background Escherichia coli like other Enterobactericeae are frequent isolates implicated in food-borne diseases. The prevalence of multi-drug resistant E. coli isolates from street vended fruits and its salad is on the increase across Nigeria. This study was designed to evaluate the antimicrobial activity Tetrapleura tetraptera (Uyayak) against E. coli as well as the druggability and pharmacokinetics of its bioactive compounds using in-silico and in-vitro approaches. Methods We utilized previously reported standard protocols in the isolation, characterization and the identification of the isolates, and the collection and preparation of the T. tetraptera pods. Antimicrobial activity of the extracts was done using the Kirby-Bauer disc diffusion method. Resulting bioactive compounds from gas chromatography coupled to mass spectrophotometry (GC-MS) were converted into canonical stings and used to for target prediction in humans and ADMET properties using the SWISSADME and pkCSM tools. Bioactive compounds that met Lipinski’s rule of five (ROF) were subjected to molecular docking against dihydropteroate synthase of E. coli using the AutoDock vina tool and the resulting interactions visualized in 2-D via Biovia Discovery Studio 21. Results The GC-MS analysis returned a total of twenty-eight (28) bioactive compounds. The abundance of the E. coli isolates varied according to location and fruit types. At trimethoprim and extracts concentrations of < 100mg/ml and < 100% respectively, the isolates showed resistance. A total of 13 bioactive compound showed zero violations to Lipinski’s rule of five (ROF). ADMET analysis of the screened bioactive compounds showed favourable absorption (intestinal and water solubility) and toxicity (AMES and hepatoxicity) profiles than trimethoprim. Molecular docking revealed various amino residues interacting with dihydropteroate synthase and gave docking scores that ranged from − 4.0 to -5.3 kcal/mole for the bioactive compounds and − 6.5 5 kcal/mole for trimethoprim. Target prediction showed that all the bioactive are capable of reaching various targets with nuclear receptor being the most abundant target. Conclusion The bioactive compounds of T. tetraptera examined in this study showed favourable antimicrobial activity against E. coli, docking scores and pharmacokinetics, suggesting the need for further studies to validate their potential as antimetabolites for management of pathogenic E. coli infections.

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“…Honey's bioactive components have been connected to its health-promoting and nutritional properties. Honey seems to have antioxidant, anticancer, anti-inflammatory, antimicrobials well as antidiabetic, anti-obesity, and woundhealing properties [3][4][5][6][7][8][9][10] and have been used and proposed against viral diseases such as the COVID-19 virus [11,12]. In animal and human models, honey has effects on the cardiovascular system and has been revived as a therapy for gastrointestinal diseases, and asthma [13][14][15].…”

Section: Introductionmentioning

confidence: 99%

Sidr Honeys Physical and Chemical Characterization, a Comprehensive Approach through LC-MS/MS, NMR, and GC-MS Analysis

El-Wahed,

Rashwan,

AlAjmi

et al. 2023

Separations

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Honey intake is advantageous to human health due to its antioxidant, anticancer, anti-inflammatory, and antimicrobial properties, all of which are attributed to the rich bioactive compound contents. Moreover, hepatoprotective, wound healing, and gastrointestinal protective properties have been documented. Honey’s nutritional value is significantly affected by its chemical composition, which varies depending on botanical and geographical origin. In particular, after Manuka honey, Sidr honey from the Ziziphus species is the most popular. The chemical compositions, physicochemical properties, bioactive compounds, and sensory characteristics of two Sidr honey samples from Egypt and Saudi Arabia were investigated in the current study. Moisture content, electrical conductivity (EC), pH, free acidity (FA), total acidity, lactone hydroxymethylfurfural (HMF) content, and diastase (α-amylase) activity were measured. By using high-performance liquid chromatography (HPLC), mass spectrometry (LC-MS/MS), nuclear magnetic resonance (1HNMR), and solid-phase micro-extraction (SPME) coupled with gas chromatography (GC-MS) analyses, the sugar profile, non-volatile, and volatile compounds were also identified. The physicochemical analysis revealed the following results for Sidr honey from Saudi Arabia and Egypt, respectively: a moisture content of 18.03 ± 0.05% and 19.03 ± 0.06%, EC values of 1.18 ± 0.05 and 1.16 ± 0.01 mS/cm, pH values of 4.87 ± 0.08 and 5.10 ± 0.01, FA of 37.50 ± 0.05 and 36.50 ± 0.05 meq/kg, total acidity of 41.06 ± 0.05 and 37.50 ± 0.05 meq/kg, lactone of 3.49 ± 0.005 and 1 ± 0.0 meq/kg, HMF of 20.92 ± 0.02 and 11.33 ± 0.01 mg/kg, and diastase of 59.97 ± 0.05 and 8.64 ± 0.06g/100 g. Honey from Saudi Arabia and Egypt displayed 22.51 ± 0.05 and 26.62 ± 0.16 % glucose, 40.33 ± 0.06 and 35.28 ± 0.01% fructose, 8.94 ± 0.17, and 8.87 ± 0.01% sucrose, and 8.22 ± 0.006 and 8.13 ± 0.01% maltose, respectively. According to the International Honey Commission (IHC) and GCC Standardization Organization (GSO) regulations, the levels of glucose, fructose, sucrose, and maltose were near the standard levels. Flavonoids, sugars, vitamins, and nitrogen contents were additionally measured using LC-MS/MS, whereas GC-MS was employed to identify aldehydes, ketones, phenols, acids, esters, anthraquinone, hydrocarbons, and nitrogenous compounds. The results of a study on the effect of honey’s geographic origin on its broad quality are summarized. As a result, knowing its optimal chemical and physical characteristics served as the criterion and indicator of the honey’s quality.

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Antimicrobial analysis of honey against Staphylococcus aureus isolates from wound, ADMET properties of its bioactive compounds and in-silico evaluation against dihydropteroate synthase

Cited by 11 publications

References 45 publications

Antibacterial activities of honey against Escherichia coli and Staphylococcus aureus: A potential treatment for bacterial infections and alternative to antibiotics

Antibacterial activities of honey against Escherichia coli and Staphylococcus aureus: A potential treatment for bacterial infections and alternative to antibiotics

Antimicrobial activity of Tetrapleura tetraptera (Uyayak) extracts, ADMET profiling and molecular docking of its bioactive compounds against dihydropteroate synthase of Escherichia coli

Sidr Honeys Physical and Chemical Characterization, a Comprehensive Approach through LC-MS/MS, NMR, and GC-MS Analysis

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