Antimicrobial activity of the polyphenolic fractions derived from Grewia asiatica, Eugenia jambolana and Carissa carandas

Abstract

Crude methanolic extracts from Grewia asiatica, Eugenia jambolana and Carissa carandas were separated into four major fractions viz. phenolic acids, flavanols, flavonols and anthocyanins which were then analysed for their total phenolic, flavonoid contents, and antimicrobial effects. In addition, anthocyanin fraction was also analysed for total anthocyanins, total colour and polymeric pigments. Total phenolics and flavonoids were highest in the fractions from E. jambolana and lowest in C. carandas, the order being phenolic acid > flavanols > flavonols > anthocyanins in all fruits. All fractions showed significant antibacterial activity except anthocyanin. Being the most active, phenolic acid fractions were also tested for their antifungal activity, the fractions of C. carandas and G. asiatica substantially inhibited all the tested fungal species. These results are being reported for the first time.

Polyphenols and Herbal-Based Extracts at the Basis of New Antioxidant, Material Protecting Products

“…• methoxy groups drastically decrease the antibacterial activity of flavonoids. Other studies [43] on some crude methanolic extracts isolated from Grewia asiatica, Eugenia jambolana and Carissa carandas separated, each one, into four major fractions respectively, 1)phenolic acids, 2)flavanols, 3)flavonols and 4)anthocyanins fractions indicated that, besides being the most active on microbial strains, phenolic acid fractions also inhibited all tested fungal species. Similarly, studies on Tunisian Quince (Cydonia oblonga Miller) pulp and peel polyphenolic extracts [44], shown as very reach in caffeoyl derivates demonstrated that chlorogenic acids acts in synergism with other components of the extracts to exhibit their total antimicrobial activities.…”

Nutritional and medicinal potential of Grewia subinaequalis DC. (syn. G. asiatica.) (Phalsa)

“…Anthocyanin, flavonoid and total phenolic contents (data expressed as milligrams per 100 g of weight). (Siddiqi et al, 2011 ( Siddiqi et al, 2013). All the fractions showed potent radical-scavenging activity.…”

“…The order of antioxidant activity of the different fractions were-Fraction Ib > Fraction Ic > Fraction II > Fraction Ia. The role of polyphenols as radical scavengers and in increasing the resistance of LDL oxidation involved in heart diseases have been demonstrated by many in vitro studies Antioxidant activity in the fruit of G. asiatica (Phalsa) can be explained on the basis of total phenolic contents, flavonoids and anthocyanins (Table 6) (Siddiqi et al, 2011). The order of antioxidant activity of the different fractions-Fraction Ib > Fraction Ic > Fraction II > Fraction Ia.…”

Nutraceutical perspectives and value addition of phalsa (Grewia asiaticaL.): A review

“…The number of spermatogina "A", spermatogonia "B", spermatocytes and spermatid count were improved in the Grewia asiatica extract group significantly as compare to non-treated Grewia asiatica group in the mice for 30 days Singh et al 2007Grewia asiatica extract increase LPO and decrease GSH level in gamma radiation exposure mice brain Sharma and Sisodia (2010) The fruit protect against radiation-induced alterations in blood of Swiss albino mice Sharma et al 2011Grewia asiatica extract exhibit strong radio protective effect in protein carbonyl assay along with strong radical scavenging activity Table 2) (Abou Zeid, Mohammed, & Sleem, 2015;Akhtar et al, 2018;Babu et al, 2017;Islary et al, 2016;Khanal et al, 2016;Khatune et al, 2016;Sharma & Sisodia, 2009;Srivastava et al, 2012;Shukla et al, 2016a;Siddiqi, Naz, Ahmad, & Sayeed, 2011;Tiwari et al, 2014).…”