Antimicrobial Activity of the Methanolic and Crude Alkaloid Extracts of Acalypha wilkesiana cv. macafeeana Copper Leaf

Ezekiel, Chibundu N.; Anokwuru, Chinedu P.; Nsofor, E.; Odusanya, O. A.; Adebanjo, O.O.

doi:10.3923/jm.2009.269.277

Cited by 27 publications

(22 citation statements)

References 7 publications

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“…The efficiency of methanol in the extraction of phytochemicals has been reported in other studies [13,21,22]. Our results seem to be consistent with others confirming methanol as a good solvent for extraction of bioactive compounds from plants as it gave the highest yield in all five plants studied.…”

Section: Resultssupporting

confidence: 92%

Aqueous and Organic Solvent-Extracts of Selected South African Medicinal Plants Possess Antimicrobial Activity against Drug-Resistant Strains of Helicobacter pylori: Inhibitory and Bactericidal Potential

Njume

Jide

Ndip

2011

IJMS

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The aim of this study was to identify sources of cheap starting materials for the synthesis of new drugs against Helicobacter pylori. Solvent-extracts of selected medicinal plants; Combretum molle, Sclerocarya birrea, Garcinia kola, Alepidea amatymbica and a single Strychnos species were investigated against 30 clinical strains of H. pylori alongside a reference control strain (NCTC 11638) using standard microbiological techniques. Metronidazole and amoxicillin were included in these experiments as positive control antibiotics. All the plants demonstrated anti-H. pylori activity with zone diameters of inhibition between 0 and 38 mm and 50% minimum inhibitory concentration (MIC50) values ranging from 0.06 to 5.0 mg/mL. MIC50 values for amoxicillin and metronidazole ranged from 0.001 to 0.63 mg/mL and 0.004 to 5.0 mg/mL respectively. The acetone extracts of C. molle and S. birrea exhibited a remarkable bactericidal activity against H. pylori killing more than 50% of the strains within 18 h at 4× MIC and complete elimination of the organisms within 24 h. Their antimicrobial activity was comparable to the control antibiotics. However, the activity of the ethanol extract of G. kola was lower than amoxicillin (P < 0.05) as opposed to metronidazole (P > 0.05). These results demonstrate that S. birrea, C. molle and G. kola may represent good sources of compounds with anti-H. pylori activity.

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Section: Resultssupporting

confidence: 92%

Aqueous and Organic Solvent-Extracts of Selected South African Medicinal Plants Possess Antimicrobial Activity against Drug-Resistant Strains of Helicobacter pylori: Inhibitory and Bactericidal Potential

Njume

Jide

Ndip

2011

IJMS

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“…However aqueous extracts of C. holstii had higher yields than that of organic extracts of a similar plant. The productivity of methanol in the extraction of phytochemicals has been reported in other studies (Ezekiel et al 2009). These results appeared to be consistent with others confirming methanol as a decent solvent for extraction of bioactive mixtures from plants as it gave the most elevated yield in three studied plants.…”

Section: Percentage Yield Of the Plant Extractssupporting

confidence: 68%

Antimicrobial activity, toxicity and phytochemical screening of selected medicinal plants of Losho, Narok County, Kenya

et al. 2017

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Chalo DM, Lukhoba C, Fidahussein DS, Nguta JM. 2017. Antimicrobial activity, toxicity and phytochemical screening of selected medicinal plants of Losho, Narok County, Kenya. Biofarmasi (Rumphius J Nat Prod Biochem) 15: 29-43. In Kenya, microbial infections are a major cause of morbidity. The effectiveness of antibiotic is threatened by the increase of resistance of pathogenic microbes against most available drugs because new pathogens continue to emerge. Nowadays, herbal remedies offer hope since they are readily available and cheap. The aim of this research was to investigate the activity of antimicrobial, the lethality of brine shrimp and phytochemical composition of crude extracts of four selected plants namely Schrebera alata (Oleaceae), Ormocarpum kirkii (Papilionoideae), Helichrysum forskahlii (Asteraceae) and Cussonia holstii (Araliaceae) that are medicinally used by herbalists from Losho, Narok County Kenya for treatment of ear, nose and throat infections, gastrointestinal disorders and skin diseases. Qualitative antimicrobial susceptibility test against five microorganisms, methicillin-resistant Staphylococcus aureus, Bacillus cereus, Escherichia coli, Pseudomonas aeruginosa and Candida albicans was investigated using agar diffusion methods to produce inhibition zones and the data accrued were analyzed using Analysis of variance. Minimum inhibitory concentrations were determined by broth microdilution method. Toxicity of the extracts was analyzed using brine shrimp lethality assay. The median fatal concentration of fifty was determined by data analysis using Finneyâ€Ÿs computer program. Phytochemical screening for flavonoids, sterols, alkaloids, tannins, quinones and terpenoids and saponins was determined using standard procedures. The observation showed that the organic crude extracts of H. forskahlii had the highest inhibition zone against methicillin-resistant S. aureus of 19.5 and 18.5 mm in agar well and agar disk diffusion respectively. In addition, organic extracts of H. forskahlii showed the highest antifungal inhibition zone of 8.5 mm in agar well diffusion. Minimum values of inhibitory concentrations varied from 15.625 to 250 mg/mL. Organic crude extracts of H. forskahlii and C. holstii were found to be highly toxic with lethal concentration of 0.009 mg/mL. All plant crude extracts contained flavonoids, sterols, alkaloids, tannins, quinones, and terpenoids. Saponins were present in all the plant extracts except in organic extracts of H. forskahlii. This study promoted the first record of antimicrobial activity, toxicity and phytochemical composition of S. alata and C. holstii. The study has shown that H. forskahlii and O. kirkii possess promising antimicrobial activity against microbes of health importance and could lead to the isolation of new, safe and efficacious antimicrobial compounds. Further research should be carried on O. kirkii and S. alata to isolate and characterize the compounds responsible for the observed activity.

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“…It is a native of tropical Asia that has been introduced to many tropical countries 4 . Investigations have been carried out with respect to the phytochemical and medicinal uses of Acalypha wilkesiana [4][5][6][7][8][9] . Consequently, the plant has been reported to have antimicrobial properties 8,10 .…”

Section: Introductionmentioning

confidence: 99%

“…Investigations have been carried out with respect to the phytochemical and medicinal uses of Acalypha wilkesiana [4][5][6][7][8][9] . Consequently, the plant has been reported to have antimicrobial properties 8,10 . Some of these studies have confirmed that the nature of the solvent and extraction process employed plays very crucial roles in the phytochemicals present in an extract, hence their medicinal properties.…”

Section: Introductionmentioning

confidence: 99%

Evaluation of Antimicrobial Properties of Herbal Ointments Formulated With Ethanolic Extract of Acalypha Wilkesiana

Azubuike¹,

Igbokwe²,

Essien³

et al. 2013

J Biol Sci Opin

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The antimicrobial activity of ethanolic extract of dried fresh leaves of Acalypha wilkesiana and also those of the ointments formulated with the extract was evaluated. The preliminary in vitro antimicrobial activity of the extract at concentrations of 2.5 mg/ml, 5 mg/ml and 10 mg/ml and those of the ointments was determined against MIC values of the extract against Escherichia coli, Staphylococcus aureus, Pseudomonas aeruginosa, andCandida albicans were 5.00, 1.25, 10.00 and 5.00 mg/ml respectively. The formulation containing Acalypha wilkesiana extract in emulsifying ointment showed better antibacterial activity than the simple ointment. The herbal ointment also compared favourably with a commercial brand of Funbact A ® cream. This study shows that ethanolic extract of Acalypha wilkesiana has both antibacterial and anti-candidal activity and also has high potential as antimicrobial agent when formulated as ointment for topical use.

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Antimicrobial Activity of the Methanolic and Crude Alkaloid Extracts of Acalypha wilkesiana cv. macafeeana Copper Leaf

Cited by 27 publications

References 7 publications

Aqueous and Organic Solvent-Extracts of Selected South African Medicinal Plants Possess Antimicrobial Activity against Drug-Resistant Strains of Helicobacter pylori: Inhibitory and Bactericidal Potential

Aqueous and Organic Solvent-Extracts of Selected South African Medicinal Plants Possess Antimicrobial Activity against Drug-Resistant Strains of Helicobacter pylori: Inhibitory and Bactericidal Potential

Antimicrobial activity, toxicity and phytochemical screening of selected medicinal plants of Losho, Narok County, Kenya

Evaluation of Antimicrobial Properties of Herbal Ointments Formulated With Ethanolic Extract of Acalypha Wilkesiana

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