Antimicrobial Activity of Tea Extracts on Cariogenic Bacterium (Streptococcus mutans)

Yoshino, Kyoji; Nakamura, Yoshiyuki; Ikeya, Hirotoshi; Sei, Tomomi; Inoue, Aya; Sano, Mitsuaki; Tomita, Isao

doi:10.3358/shokueishi.37.104

Cited by 21 publications

(18 citation statements)

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“…[9,[13][14][15][16] Briefly, S. mutans was grown overnight in YN with glucose. After 16 hours 0.1 mL of the stationary phase culture from the broth was transferred to a culture tube that contained 15 mL of the broth.…”

Section: Determination Of Antimicrobial Activity (Plate Dilution Assay)mentioning

confidence: 99%

Antimicrobial and Antioxidant Activities of Polyphenols against Streptococcus mutans

Sendamangalam

Choi

Seo

et al. 2011

Free Radicals and Antioxidants

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Natural polyphenols, gallic acid, tannic acid, quercetin and salicylic acid, were investigated for their antimicrobial and antioxidant activities against Streptococcus mutans. Ascorbic acid, well known for its antimicrobial and antioxidant activities, was used as a criterion for the polyphenols. The antimicrobial effect was assessed using the plate dilution assay and the minimum inhibitory concentration (MIC) of each polyphenol was then determined from the antimicrobial activity results. Salicylic acid was the weakest antimicrobial with the highest MIC (3.8 mg/mL), and tannic acid was the strongest antimicrobial with the lowest MIC of 0.4 mg/mL. Antioxidant capacities were evaluated using the DMPD and ABTS decolorizing assays. These polyphenols show high antimicrobial activity and inoxidizability. Antioxidant activity for quercetin according to the DMPD method was inconclusive because it had color interference with the DMPD radicals. Although some conflicting results were observed between the DMPD and ABTS methods, the polyphenols with high antioxidant capacities still showed high antimicrobial activities, which suggest that the antioxidant capacity attributes to the antimicrobial effects.

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Section: Determination Of Antimicrobial Activity (Plate Dilution Assay)mentioning

confidence: 99%

Antimicrobial and Antioxidant Activities of Polyphenols against Streptococcus mutans

Sendamangalam

Choi

Seo

et al. 2011

Free Radicals and Antioxidants

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“…There have been reports that BTND exhibited an anti-tumor-promoting effect in mouse epidermal JB6 cell lines, an inhibitory effect on duodenal carcinogenesis in mice (15,17), induction of apoptotic cell death in cancer cells (18), and an antibacterial action (14). Moreover, it has been considered that the active ingredients of BTND are polyphenolic compounds of flavonols and gallates, quinic acid and carbohydrates of D-glucose and Dgalactose units (15).…”

Section: Discussionmentioning

confidence: 99%

“…For example, tea aqueous non-dialysate (TND) is the generic name of the fraction of water-soluble polymer components with molecular weights over 12000 extracted from tea leaves. It has already been reported that black tea aqueous non-dialysate (BTND), which is well characterized among several kinds of TND, exhibits anti-microbial activity against a cariogenic bacterium (14). Therefore, the activity of BTND against H. pylori was investigated in the present study.…”

Section: Introductionmentioning

confidence: 99%

Effect of black tea aqueous non-dialysate onHelicobacter pylori infection in Mongolian gerbils

Takabayashi¹,

Nakamura²,

Harada³

2004

Environ Health Prev Med

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Objectives: Recently, the appearance of Helicobacter pylori (H. pylori) resistant to antibiotics has been reported. The development of an antibiotic therapy which would not induce resistant strains of H. pylori is anticipated. In the present study, the antibiotic effect of black tea aqueous non-dialysate (BTND), the fraction different from tea catechins, on H. pylori was investigated using Mongolian gerbils infected with H. pylori.Methods: BTND was extracted from black tea leaves. A 0.1 w/v% solution of BTND or green tea catechins (GTC) was provided as drinking water to male NGS/Sea Mongolian gerbils infected with H. pylori (ATCC43504) for two weeks. Their stomachs were then excised, the mucosal surfaces were macroscopically observed, and colony forming units (CFU) of H. pylori were counted. The data were compared between the BTND and GTC groups.Results: The CFU of H. pylori were significantly decreased by intake of BTND. The body weight of the animals tended to be larger in the group supplied with BTND than in that supplied with GTC. Gastric mucosal injury tended to be smaller in the animals supplied with BTND than in those with GTC.Conclusions: These results suggest that BTND may have an inhibitory effect on H. pylori infection.

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“…In fact, polyphenols present in green tea contain high levels of catechins and theaflavins and have very strong antibacterial properties. 50 Some other studies have evaluated the anticariogenic properties of green tea by assessing its direct effect on microorganisms. 51 In vitro studies have shown that catechins present in green tea prevent adhesion of Streptococci to tooth surfaces.…”

Section: Discussionmentioning

confidence: 99%

Effect of Green Tea Varnish on Depth of Root Caries

Daneshyar

Khamverdi

Toliat

et al. 2018

The Journal of Contemporary Dental Practice

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Aim: Root caries is a common, debilitating condition particularly in the elderly population, which can lead to tooth loss. Evidence shows that green tea has cariostatic effects. Considering the gap of information on green tea varnish, this study aimed to assess the efficacy of green tea varnish in the prevention of root caries. Materials and methods:This in vitro experimental study was performed on 42 sound premolars. Two layers of acid-resistant nail varnish were applied on root surfaces except for a window of 1 × 4 mm. The teeth were randomly divided into three groups (n = 14). Group I (control) received no intervention. Group II received green tea varnish applied on the roots every 48 hours for 21 days. Group III received green tea varnish every 24 hours for 21 days. Sections of 40 µ thickness were prepared from the center of the window, and the depth of carious lesion was measured in three points with 500 µ distance from each other using polarized light microscope. Data were analyzed using Statistical Package for the Social Sciences (SPSS) version 16 and nonparametric Kruskal-Wallis and Mann-Whitney U tests (α = 0.05). Results:The mean (and standard deviation) depth of carious lesion was 54.30 ± 28.64 (µm), 0, and 0 in groups I, II, and III respectively. Control group showed the highest depth of caries. Groups II and III were not significantly different in this respect, but significant differences were noted in depth of caries between groups I and II and also groups I and III (p < 0.001). Clinical significance: Application of green tea varnish can prevent root caries. Conclusion

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Antimicrobial Activity of Tea Extracts on Cariogenic Bacterium (Streptococcus mutans)

Cited by 21 publications

References 0 publications

Antimicrobial and Antioxidant Activities of Polyphenols against Streptococcus mutans

Antimicrobial and Antioxidant Activities of Polyphenols against Streptococcus mutans

Effect of black tea aqueous non-dialysate onHelicobacter pylori infection in Mongolian gerbils

Effect of Green Tea Varnish on Depth of Root Caries

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