Antimicrobial activity of seaweeds extracts against multiresistant pathogens

Shanmughapriya, Santhanam; Manilal, Aseer; Sujith, S.; Selvin, Joseph; Kiran, George Seghal; Natarajaseenivasan, Kalimuthusamy

doi:10.1007/bf03175554

Cited by 127 publications

(73 citation statements)

References 20 publications

(20 reference statements)

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“…The mechanism of antibiosis of the soap samples was calculated using the ratio of MBC or MIC or MIC index as described by Shanmughapriya et al (2008) to elucidate whether the observed antibacterial effects were bactericidal or bacteriostatic. Although antimicrobials are usually regarded as bactericidal if the MBC is no more than four times the MIC (French 2006), when the ratio of MBC or MIC was ≤ 2.0, the soap samples were considered bactericidal or otherwise bacteriostatic.…”

Section: Determination Of Mechanisms Of Antibiosis (Bactericidal or Bmentioning

confidence: 99%

A comparison of the antibacterial activity of some African black soaps and medicated soaps commonly used for the treatment of bacteria-infected wound

Olajuyigbe

Adeoye-Isijola

Adedayo

2017

JOMPED

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Background: Black soap is a medicinal product that could be harnessed for economic purpose if properly packaged, and misconception about its traditional use by herbalists is thrown overboard.Aims: To promote the relevance of these soaps for economic development, this study compared the antibacterial activity of black soaps with medicated soaps widely used against bacterial infections.Methods: The antibacterial activities of these soap samples were determined by agar diffusion and macrobroth dilution methods.Results: In this study, the statistical analysis of the inhibition zones showed that black soaps were significantly (p < 0.05) more active than medicated soaps used against the test bacterial isolates. The black soaps inhibited and killed the isolates better than the medicated soaps at the different concentrations used. The minimum inhibitory concentration for Klebsiella pneumoniae and Enterococcus faecalis ranged between 0.125 mg/mL and 2 mg/mL, Staphylococcus aureus (0.25–4) mg/mL, Escherichia coli (0.125–4) mg/mL and Pseudomonas aeruginosa (1–4) mg/mL. The result showed that K. pneumoniae and E. faecalis were the most susceptible, followed by E. faecalis > E. coli > S. aureus > P. aeruginosa.Conclusion: As a valuable medicinal output derivable from organic waste product that could be converted to wealth, African black soap production, utilisation and commercialisation have tremendous economic potentials. These soaps showed significant antibacterial activity greater than those of the medicated soaps. Hence, their use could be a better option in place of commercially available medicated and antiseptic soaps because of the degree of antibacterial activities they exhibited.

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Section: Determination Of Mechanisms Of Antibiosis (Bactericidal or Bmentioning

confidence: 99%

A comparison of the antibacterial activity of some African black soaps and medicated soaps commonly used for the treatment of bacteria-infected wound

Olajuyigbe

Adeoye-Isijola

Adedayo

2017

JOMPED

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“…The mechanism of antibiosis of the extract was calculated using the ratio of MBC/MIC or MFC/MIC or MIC index as described by Shanmughapriya et al (2008) to elucidate whether the observed antibacterial effects were bactericidal or bacteriostatic. When the ratio of MBC/MIC or MFC/MIC was ≤ 2.0, the extract was considered bactericidal/fungicidal or otherwise bacteriostatic/fungistatic.…”

Section: Determination Of Mechanisms Of Antibiosis (Bactericidal or Bmentioning

confidence: 99%

GC-MS analysis and antimicrobial effects of methanol stem bark extract of Trilepisium madagascriense DC.

Olajuyigbe¹,

Faith²,

Adeoye-Isijola³

2017

ijSciences

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ABSTRACT:The therapeutic potentials of methanol stem bark extract of Trilepisium madagascriense was determined using Gas Chromatography-Mass Spectrometry to identify its bioactive compounds of pharmaceutical importance while the antimicrobial activities were assayed in vitro by agar well diffusion and macrobroth dilution techniques against different microbial isolates. The mass spectra of the identified compounds in the extract at different retention time showed the presence of ethyl iso-allocholate, (3β,5Z,7E)-9,10-Secocholesta-5,7,10(10)-triene-3,24,25-triol, 2,6-Dimethoxyamphetamine, 4-Hexenoic acid, 4-methyl-6-(fluorodimethylsilyl)-6-trimethysily-, 2-methoxy-4-(methoxymethyl)-Phenol, 2-methoxy-1,4-Benzenediol, 2,4-Dimethoxyphenol, Indole, Paromomycin, Hydroquinone and Tetrahydro-N-[(tetrahydro-2-furanyl)methyl-2-Furanmethanamine amongst other bioactive compounds of therapeutic potentials. This extract showed antimicrobial activities. At the lowest concentration of 25 mg/ml, 100 µl of the extract produced inhibition zones ranging between 14 and 18 ± 1.0 mm and inhibition zones ranging between 18 and 28 ± 1.0 mm in all the isolates at the highest concentration of 100 mg/ml. While the bacterial MICs ranged between 1.25 and 5 mg/ml and the MBCs ranged between 2.5 and 10 mg/ml, the fungal MICs ranged between 0.098 and 12.5 mg/ml while the MFCs ranged between 0.781 and <25 mg/ml. With exception of MIC index of Klebsiella pneumoniae ATCC 10031 which was equal to 4, the MIC index of other isolates ranged between 1 and 2. Klesbiella pneumoniae ATCC 10031 and Proteus vulgaris CSIR 0030 had the highest MICs of 1.25 mg/ml, followed by B. cereus ATCC 10702, Staphylococcus aureus ATCC 6538, Pseudomonas aeruginosa ATCC 19582, Enterococcus faecalis ATCC 29212 and Bacillus subtilis KZN with MICs of 2.5 mg/ml while Escherichia coli ATCC 25922, Enterococcus cloacae ATCC 13047, Enterococcus faecalis KZN, Shigella sonnei ATCC 29930, Klebsiella pneumoniae KpFa, Staphylococcus aureus SaFa, Escherichia coli EcFa and Pseudomonas aeruginosa PmFa had the least MICs of 5.0 mg/ml. Enterococcus faecalis KZN, Bacillus subtilis KZN and Proteus vulgaris CSIR 0030 had the highest MBCs of 2.5 mg/ml. Although Candida albicans had MICs ranging between 0.098 mg/ml and Candida tropicalis had the least MICs of 12.5 mg/ml, the MFCs were 0.781 mg/ml and 25 mg/ml. This study shows that the pharmacological effects of Trilepisium madagascriense depends on bioactive compounds identified while this plant is a source for isolating novel drugs having significant therapeutic potentials.

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“…If ratio of MBC/MIC was d" 2, the active crude extract was considered as bactericidal; if not, it was considered as bacteriostatic. If the ratio was e"16, then the active crude extract is considered to be ineffective 22 .…”

Section: Determination Of Minimum Bactericidal Concentrations (Mbc)mentioning

confidence: 99%

Phytochemical Composition and In vitro Antimicrobial, Antioxidant Activites of Methanolic Leaf Extracts From Excoecaria agallocha

A.G

et al. 2016

Biosci., Biotech. Res. Asia

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This study investigated the phytochemical, antimicrobial and antioxidant properties of methanolic leaf fractionated extracts of Excoecaria agallocha. Antibacterial activity test of fractionated extract of Excoecaria agallocha was conducted via Disk diffusion assay. Micro titer broth dilution technique was performed to determine the minimum inhibitory concentration (MIC) and Minimum Bactericidal Concentration (MBC). Qualitative and quantitative DPPH radical scavenging activity determines the antioxidant activity in E. agallocha. Chromatographic separation was completed using thin layer chromatography (TLC) and column chromatographic techniques. Phytochemical screening of fractionated extract revealed active constituents including alkaloids, saponins, flavonoids, terpenoid and tannins. In vitro antibacterial activity of columnfractionated extract demonstrated inhibitory effect against Flavobacterium indicum, Chryseobacterium meningosepticum, Chryseobacterium indologenes and Chryseobacterium gleum isolated from diseased fish. MIC and MBC of E. agallocha fractionated extract was 1.56 mg/ml and 3.12 mg/ml, respectively. TLC-based qualitative DPPH analysis revealed antioxidant activity in E. agallocha extract, specified by yellowish stain on maroon color TLC plate. These bands indicate the presence of various compounds that could act as potential antimicrobial agents. The present study indicates that E. agallocha leaves contain active phytochemicals which inhibit bacteria and could be useful in the treatment of microbial infections in fish.

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Antimicrobial activity of seaweeds extracts against multiresistant pathogens

Cited by 127 publications

References 20 publications

A comparison of the antibacterial activity of some African black soaps and medicated soaps commonly used for the treatment of bacteria-infected wound

A comparison of the antibacterial activity of some African black soaps and medicated soaps commonly used for the treatment of bacteria-infected wound

GC-MS analysis and antimicrobial effects of methanol stem bark extract of Trilepisium madagascriense DC.

Phytochemical Composition and In vitro Antimicrobial, Antioxidant Activites of Methanolic Leaf Extracts From Excoecaria agallocha

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